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STUDYING GENETIC EFFECTS IN NATURAL ECOSYSTEMS WITH APPLICATION OF A PLANT TEST OBJECT

https://doi.org/10.30901/2227-8834-2015-3-346-356

Abstract

The genotoxicity and clastogenic effects of water samples from Lake Sevan and the rivers of its basin with the application of Trad-SF (stamen filaments) and Trad-MN (micronuclei in the tetrads of microspores) bioassays of Tradescantia (clone 02) were investigated. A significant increase in the level of recessive mutation events (RME) frequency and micronuclei (MN) in tetrads from the water samples from the Gavaraget and Dzknaget Rivers as compared with the reference and other studied samples was detected. High positive correlation between the RME and MN frequencies and the concentration of some chemical elements in the studied water samples (Si, Al, P, Mn, Fe, and Cu) were revealed. The obtained results show that Trad-SF and Trad-MN tests of Tradescantia (clone 02) can be used for ecotesting of natural aquatic ecosystems in Armenia.

About the Authors

E. A. Aghajanyan
Yerevan State University
Russian Federation


R. E. Avalyan
Yerevan State University
Russian Federation


A. L. Atoyants
Yerevan State University
Russian Federation


A. E. Simonyan
Yerevan State University
Russian Federation


R. M. Aroutiounian
Yerevan State University
Russian Federation


References

1. Агаджанян Э. А., Атоянц А. Л., Варжапетян А. С., Авалян Р. Э., Арутюнян Р. М. Оценка загрязненности почв г. Еревана тяжелыми металлами // Ученые записки ЕГУ. 2011. № 1. С. 44-48.

2. Акопян К. А. Антропогенная нагрузка на водные ресурсы и их безопасность // Материалы Междунар. конференции РЭЦ Кавказ «Экологическая безопасность Кавказского региона». 2004. Вып. 7. С. 126-128.

3. Большаков В. Н., Лущекина А. А., Неронов В. М. Сохранение биологического разнообразия: от экосистемы к экосистемному подходу // Экология. 2009. № 2. С. 83-90.

4. Grant W. F., Lee H. G., Logan D. M., Salamone M. F. The use of Tradescantia and Vicia faba bioassays for the in situ detection of mutagens in an aquatic environment // Mutation Research. 1992. Vol. 270. P. 53-64.

5. Ma T. H., Cabrera G. L., Chen R., Gill B. S., Sandhu S. S., Vandenberg A. L., Salamone M. F. Tradescantia Micronucleus Bioassay // Mutation Research. 1994b. Vol. 310, N 2. P. 220-230.

6. Ma T. H., Cabrera G. L., Cebulska-Wasilevska A., Chen R., Loarca F., Vandenberg A L., Salamone M. F. Tradescantia stamen hair mutation bioassay // Mutation Research. 1994a. Vol. 310. N 2. P. 211-220.

7. Оганесян Р. О. Озеро Севан вчера, сегодня ... . Ереван : Гитутюн НАН РА, 1994. 478 с.

8. Осипова Р. Г., Шевченко В. А. Использование традесканции (клоны 02 и 4430) в исследованиях по радиационному и химическому мутагенезу // Журнал Общей Биологии. 1984. Т. XLV, № 2. С. 226-232.

9. Pogosyan V. S., Aghajanyan E. A., Atoyants A. L. Mutagenisity of ground water (in the bore-holes) in Ararat Valley (Armenia) detected by Trad-SHM bioassay // Mutation Research. 2002. Vol. 518. P. 151-153.


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For citations:


Aghajanyan E.A., Avalyan R.E., Atoyants A.L., Simonyan A.E., Aroutiounian R.M. STUDYING GENETIC EFFECTS IN NATURAL ECOSYSTEMS WITH APPLICATION OF A PLANT TEST OBJECT. Proceedings on applied botany, genetics and breeding. 2015;176(3):346-356. (In Russ.) https://doi.org/10.30901/2227-8834-2015-3-346-356

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ISSN 2227-8834 (Print)
ISSN 2619-0982 (Online)