In silico analysis of soybean genomic regions associated with seed protein content
https://doi.org/10.30901/2227-8834-2026-2-o4
Abstract
Soybean (Glycine max (L.) Merr.) is one of the most important crops in global agriculture. Due to its high protein content, soybean is actively cultivated in many countries. Upon reviewing published sources and analyzing available online resources containing whole-genome data for different cultivars, the sequences of genes encoding asparagine synthetase (AS) and asparaginase (ASPG) proteins associated with protein content in soybean seeds were analyzed. Multiple sequence alignments were performed for the target sequences in cvs. ‘Jack’, ‘Enrei’, ‘Williams 82’, and others, revealing SNPs and InDels. Primers were designed for standard PCR and DNA barcoding. Additionally, a panel of 23 soybean accessions from the VIR collection was selected, exhibiting contrasting protein content, which can be used for further studies and validation.
Supporting agencies: this study was conducted with financial support from the state program for the Sirius Federal Territory “Scientific and technological development of the Sirius Federal Territory” (Agreement No. 18-03 dated Sept. 10, 2024). Soybean accessions were sampled within the framework of the thematic plan of VIR, Project No. FGEM-2022-0002.
About the Authors
I. V. Rozanova
Sirius University of Science and Technology, Research Center of Genetics and Life Sciences
Russian Federation
Russian Federation
Irina V. Rozanova, Cand. Sci. (Biology), Senior Researcher
1 Olimpiysky Ave., Sirius Settlem., Sirius Federal Territory, Krasnodar Territory 354340, Russia
P. V. Klimova
Sirius University of Science and Technology, Research Center of Genetics and Life Sciences
Polina V. Klimova, MSc Student
1 Olimpiysky Ave., Sirius Settlem., Sirius Federal Territory, Krasnodar Territory 354340, Russia
A. Ya., Evlash
Sirius University of Science and Technology, Research Center of Genetics and Life Sciences; N.I. Vavilov All-Russian Institute of Plant Genetic Resources
Russian Federation
Russian Federation
Anastasia Ya. Evlash, Associate Researcher
1 Olimpiysky Ave., Sirius Settlem., Sirius Federal Territory, Krasnodar Territory 354340, Russia; 42, 44 Bolshaya Morskaya Street, St. Petersburg 190000, Russia
I. V. Seferova
N.I. Vavilov All-Russian Institute of Plant Genetic Resources
Russian Federation
Russian Federation
Irina V. Seferova, Cand. Sci. (Biology), Leading Researcher, VIR
42, 44 Bolshaya Morskaya Street, St. Petersburg 190000, Russia
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For citations:
Rozanova I.V., Klimova P.V., Evlash A.Ya., Seferova I.V. In silico analysis of soybean genomic regions associated with seed protein content. Proceedings on applied botany, genetics and breeding. 2026;187(2):226-234. (In Russ.) https://doi.org/10.30901/2227-8834-2026-2-o4
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