vir-nwТруды по прикладной ботанике, генетике и селекцииProceedings on applied botany, genetics and breeding2227-88342619-0982N.I. Vavilov All-Russian Institute of Plant Genetic Resources10.30901/2227-8834-2026-2-o4vir-nw-2515Research ArticleКРАТКИЕ СООБЩЕНИЯBRIEF REPORTSАнализ in silico геномных районов сои, ассоциированных с содержанием белка в семенахIn silico analysis of soybean genomic regions associated with seed protein contenthttps://orcid.org/0000-0003-4341-0766РозановаИ. В.RozanovaI. V.

Ирина Вениаминовна Розанова, кандидат биологических наук, старший научный сотрудник

354340 Россия, Краснодарский край, федеральная территория «Сириус», пгт. Сириус, Олимпийский пр.

Irina V. Rozanova, Cand. Sci. (Biology), Senior Researcher

1 Olimpiysky Ave., Sirius Settlem., Sirius Federal Territory, Krasnodar Territory 354340, Russia

rozanova.iv@talantiuspeh.ruhttps://orcid.org/0009-0003-7808-3782КлимоваП. В.KlimovaP. V.

Полина Владимировна Климова, магистрант

354340 Россия, Краснодарский край, федеральная территория «Сириус», пгт. Сириус, Олимпийский пр., 1

Polina V. Klimova, MSc Student

1 Olimpiysky Ave., Sirius Settlem., Sirius Federal Territory, Krasnodar Territory 354340, Russia

Klimova.polya04@gmail.comhttps://orcid.org/0000-0003-0246-8929ЕвлашА. Я.EvlashA. Ya.,

Анастасия Ярославовна Евлаш, младший научный сотрудник

354340 Россия, Краснодарский край, федеральная территория «Сириус», пгт. Сириус, Олимпийский пр., 1; 190000 Россия, Санкт-Петербург, ул. Б. Морская, 42, 44

Anastasia Ya. Evlash, Associate Researcher

1 Olimpiysky Ave., Sirius Settlem., Sirius Federal Territory, Krasnodar Territory 354340, Russia; 42, 44 Bolshaya Morskaya Street, St. Petersburg 190000, Russia

evlash.ae@talantiuspeh.ruhttps://orcid.org/0000-0003-3308-9198СефероваИ. В.SeferovaI. V.

Ирина Владимировна Сеферова, кандидат биологических наук, ведущий научный сотрудник, ВИР

190000 Россия, Санкт-Петербург, ул. Б. Морская, 42, 44

Irina V. Seferova, Cand. Sci. (Biology), Leading Researcher, VIR

42, 44 Bolshaya Morskaya Street, St. Petersburg 190000, Russia

i.seferova@vir.nw.ruНаучно-технологический университет «Сириус», Центр генетики и наук о жизниРоссияSirius University of Science and Technology, Research Center of Genetics and Life SciencesRussian FederationНаучно-технологический университет «Сириус», Центр генетики и наук о жизни; Федеральный исследовательский центр Всероссийский институт генетических ресурсов растений имени Н.И. ВавиловаРоссияSirius University of Science and Technology, Research Center of Genetics and Life Sciences; N.I. Vavilov All-Russian Institute of Plant Genetic ResourcesRussian FederationФедеральный исследовательский центр Всероссийский институт генетических ресурсов растений имени Н.И. ВавиловаРоссияN.I. Vavilov All-Russian Institute of Plant Genetic ResourcesRussian Federation2026060720261872226234Copyright © Розанова И.В., Климова П.В., Евлаш А.Я., Сеферова И.В., 20262026Розанова И.В., Климова П.В., Евлаш А.Я., Сеферова И.В.Rozanova I.V., Klimova P.V., Evlash A.Y., Seferova I.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://elpub.vir.nw.ru/jour/article/view/2515

Соя (Glycine max (L.) Merr.) – один из наиболее важных объектов мирового сельского хозяйства. Благодаря высокому содержанию белка, соя активно возделывается во многих странах. На основании литературного анализа и анализа доступных онлайн-ресурсов, содержащих полногеномные данные разных сортов, были исследованы последовательности генов, которые кодируют белки аспарагинсинтетазу (AS) и аспарагиназу (ASPG), ассоциированные с содержанием белка в семенах сои. Проведено выравнивание исследуемых последовательностей в сортах ‘Jack’, ‘Enrei’, ‘Williams 82’ и др., выявлены SNP и InDel. Сконструированы праймеры для стандартной ПЦР и для ДНК-штрихкодирования. Также подобрана выборка из 23 образцов сои из коллекции ВИР, которая показала контрастное содержание белка и может быть использована в дальнейшем изучении и верификации.

Soybean (Glycine max (L.) Merr.) is one of the most important crops in global agriculture. Due to its high protein content, soybean is actively cultivated in many countries. Upon reviewing published sources and analyzing available online resources containing whole-genome data for different cultivars, the sequences of genes encoding asparagine synthetase (AS) and asparaginase (ASPG) proteins associated with protein content in soybean seeds were analyzed. Multiple sequence alignments were performed for the target sequences in cvs. ‘Jack’, ‘Enrei’, ‘Williams 82’, and others, revealing SNPs and InDels. Primers were designed for standard PCR and DNA barcoding. Additionally, a panel of 23 soybean accessions from the VIR collection was selected, exhibiting contrasting protein content, which can be used for further studies and validation.

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The authors declare that there are no conflicts of interest present.