In vitro regeneration of vegetable pea plants

Background. The objective of our research was to obtain regenerated plants of vegetable pea through tissue culture by initiating organogenesis.

Materials and methods. Two vegetable pea cultivars served as the research material. Three sterilizing agents were evaluated when processing dry and swollen seeds. The explants were the apical bud, the next node without the leaf apparatus, and the cotyledon node. Four variants of callus induction media were studied, three variants for shoot formation, and two for rhizogenesis.

Results. Callus formation was recorded for all types of explants on all variants of media. The study of the effect of phytohormones on the shoot formation in callus aggregates showed that an increase in the concentration of cytokinin leads to an increase in the number of shoot-forming calli and the number of shoots.

Conclusions. Dry seeds should be used when cultivating pea plants with wrinkled seeds in vitro, with 1% NaOCl solution as a sterilizing agent. For callus tissue production, it is recommended to use the second (NAA, 5 mg/L) or third (IBK, 6 mg/L, and 6-BAP, 1 mg/L) variants of MSC. MSS2 (6-BAP, 2.5 mg/L, and IBC, 0.5 mg/L) proved to be the optimal shoot induction environment. The media variants for rhizogenesis induction had similar effectiveness.

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