Идентификация генов – восстановителей фертильности Rf3 и Rf4 в различных генотипах риса с использованием молекулярных маркеров SSR

Актуальность. Повышение урожайности риса путем гибридизации и идентификации генов – восстановителей фертильности имеет решающее значение для повышения самообеспечения и удовлетворения потребностей в питании. Цель данного исследования – определение таких генов – восстановителей фертильности в отобранных генотипах риса с помощью SSR-маркеров.

Материалы и методы. Для идентификации генов – восстановителей фертильности в различных генотипах риса, в том числе в местных и селекционных сортах, проведен скрининг зарубежных восстановителей фертильности, усовершенствованных мутантных линий риса и двух линий с цитоплазматической мужской стерильностью (JelodarA и NematA) с использованием молекулярных маркеров. Для определения аллельного статуса основных генов – восстановителей фертильности Rf3 и Rf4 использовались шесть SSR-маркеров.

Результаты и заключение. Молекулярный анализ показал, что генотипы IR68061R, IR50, IR 68061-27-3-2-2-3R, IR 73014-59-2-2-2R, M9-P10-2-2-2-2-1, M9-P18-6-1-1-2-1, MILYANG 54, SUWEON 294, IR 9761-19-1 и IR46R содержали ген Rf4, идентифицированный тремя маркерами: RM171, RM6100 и RM228. Кроме того, наличие генов – восстановителей фертильности Rf3 и Rf4 у генотипов IR67924R, IR 57301-158-1R, M9-P12-5-3-2-2, M9-P15-6-2-1 и NSIC RC 352 подтверждено всеми маркерами. Эти генотипы признаны лучшими восстановителями фертильности для гибридной селекции риса. В целом идентификация и оценка этих генотипов может способствовать повышению производства риса и укреплению продовольственной безопасности в развивающихся странах.

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