vir-nwТруды по прикладной ботанике, генетике и селекцииProceedings on applied botany, genetics and breeding2227-88342619-0982N.I. Vavilov All-Russian Institute of Plant Genetic Resources10.30901/2227-8834-2025-4-131-141vir-nw-2274Research ArticleИДЕНТИФИКАЦИЯ ГЕНЕТИЧЕСКОГО РАЗНООБРАЗИЯ КУЛЬТУРНЫХ РАСТЕНИЙ И ИХ ДИКИХ РОДИЧЕЙ ДЛЯ РЕШЕНИЯ ФУНДАМЕНТАЛЬНЫХ И ПРИКЛАДНЫХ ПРОБЛЕМIDENTIFICATION OF THE DIVERSITY OF CULTIVATED PLANTS AND THEIR WILD RELATIVES FOR SOLVING FUNDAMENTAL AND APPLIED PROBLEMSИдентификация генов – восстановителей фертильности Rf3 и Rf4 в различных генотипах риса с использованием молекулярных маркеров SSRIdentification of fertility restorer genes Rf3 and Rf4 in diverse rice genotypes using SSR molecular markershttps://orcid.org/0000-0001-9314-0050Афхами ГадиА.Afkhami GhadiA.

Аммар Афхами Гади, доктор философии, доцент, Ардебильский научно-образовательный центр сельского хозяйства и природных ресурсов, Организация сельскохозяйственных исследований, образования и распространения знаний (AREEO); доцент, Международный университет имени имама Хомейни

57451-56951 Иран, провинция Ардебиль, Парсабад Моган, 12-й км дор. Асландоз;  34148-96818 Иран, Казвин, пр. Персидского залива, бульв. Международного университета имени имама Хомейни

Ammar Afkhami Ghadi, PhD, Assistant Professor, Ardabil Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO); Assistant Professor, Imam Khomeini International University

12th km of Aslandoz Road, Parsabad Moghan, Ardabil Province 57451-56951, Iran; Imam Khomeini International University Blvd., Persian Gulf Ave., Qazvin 34148-96818, Iran

a.afkhami@sanru.ac.irhttps://orcid.org/0000-0001-7152-896XХадемианР.KhademianR.

Рахеле Хадемиан, доктор философии, доцент, Международный университет имени имама Хомейни

34148-96818 Иран, Казвин, пр. Персидского залива, бульв. Международного университета имени имама Хомейни

Raheleh Khademian, PhD, Assistant Professor, Imam Khomeini International University,

Imam Khomeini International University Blvd., Persian Gulf Ave., Qazvin 34148-96818, Iran

r.khademian@eng.ikiu.ac.irhttps://orcid.org/0000-0002-1371-0293НематзадеГ. А.NematzadehG. A.

Горбан Али Нематзаде, доктор философии, профессор, Сарийский университет сельскохозяйственных наук и природных ресурсов

48181-66996 Иран, провинция Мазендаран, Сари, 9-й км Фарахабадской дор.

Ghorban Ali Nematzadeh, PhD, Professor, Sari Agricultural Sciences and Natural Resources University

9th km of Farahabad Road, Sari, Mazandaran Province 48181-66996, Iran

gh.nematzadeh@gmail.comhttps://orcid.org/0000-0003-3700-6724Бабаеян ДжелодарН.Babaeian JelodarN.

Надали Бабаеян Джелодар, доктор философии, профессор, Сарийский университет сельскохозяйственных наук и природных ресурсов

48181-66996 Иран, провинция Мазендаран, Сари, 9-й км Фарахабадской дор.

Nadali Babaeian Jelodar, PhD, Professor, Sari Agricultural Sciences and Natural Resources University

9th km of Farahabad Road, Sari, Mazandaran Province 48181-66996, Iran

nbabaeian@yahoo.comhttps://orcid.org/0000-0001-8012-6866БагериН.BagheriN.

Надали Багери, доктор философии, доцент, Сарийский университет сельскохозяйственных наук и природных ресурсов

48181-66996 Иран, провинция Мазендаран, Сари, 9-й км Фарахабадской дор.

Nadali Bagheri, PhD, Associate Professor, Sari Agricultural Sciences and Natural Resources University

9th km of Farahabad Road, Sari, Mazandaran Province 48181-66996, Iran

n.bagheri@sanru.ac.irАрдебильский центр исследований и образования в области сельского хозяйства и природных ресурсов, Организация сельскохозяйственных исследований, образования и распространения знаний (AREEO); Международный университет имени имама ХомейниИранArdabil Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO); Imam Khomeini International UniversityIslamic Republic of IranМеждународный университет имени имама ХомейниИранImam Khomeini International UniversityIslamic Republic of IranСарийский университет сельскохозяйственных наук и природных ресурсов, СариИранSari Agricultural Sciences and Natural Resources UniversityIslamic Republic of Iran2025271220251864131141Copyright © Афхами Гади А., Хадемиан Р., Нематзаде Г.А., Бабаеян Джелодар Н., Багери Н., 20252025Афхами Гади А., Хадемиан Р., Нематзаде Г.А., Бабаеян Джелодар Н., Багери Н.Afkhami Ghadi A., Khademian R., Nematzadeh G.A., Babaeian Jelodar N., Bagheri N.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://elpub.vir.nw.ru/jour/article/view/2274Актуальность

Актуальность. Повышение урожайности риса путем гибридизации и идентификации генов – восстановителей фертильности имеет решающее значение для повышения самообеспечения и удовлетворения потребностей в питании. Цель данного исследования – определение таких генов – восстановителей фертильности в отобранных генотипах риса с помощью SSR-маркеров.

Материалы и методы

Материалы и методы. Для идентификации генов – восстановителей фертильности в различных генотипах риса, в том числе в местных и селекционных сортах, проведен скрининг зарубежных восстановителей фертильности, усовершенствованных мутантных линий риса и двух линий с цитоплазматической мужской стерильностью (JelodarA и NematA) с использованием молекулярных маркеров. Для определения аллельного статуса основных генов – восстановителей фертильности Rf3 и Rf4 использовались шесть SSR-маркеров.

Результаты и заключение

Результаты и заключение. Молекулярный анализ показал, что генотипы IR68061R, IR50, IR 68061-27-3-2-2-3R, IR 73014-59-2-2-2R, M9-P10-2-2-2-2-1, M9-P18-6-1-1-2-1, MILYANG 54, SUWEON 294, IR 9761-19-1 и IR46R содержали ген Rf4, идентифицированный тремя маркерами: RM171, RM6100 и RM228. Кроме того, наличие генов – восстановителей фертильности Rf3 и Rf4 у генотипов IR67924R, IR 57301-158-1R, M9-P12-5-3-2-2, M9-P15-6-2-1 и NSIC RC 352 подтверждено всеми маркерами. Эти генотипы признаны лучшими восстановителями фертильности для гибридной селекции риса. В целом идентификация и оценка этих генотипов может способствовать повышению производства риса и укреплению продовольственной безопасности в развивающихся странах.

Background

Background. Enhancing rice yields through hybridization and the identification of fertility restorer genes is crucial for improving self-sufficiency and addressing nutritional needs. This study aims to explore the presence of these fertility restorer genes in selected rice genotypes using SSR markers.

Materials and methods

Materials and methods. To identify the fertility restorer gene(s) in diverse rice genotypes, including local and breeding rice varieties, foreign fertility restorers, advanced mutant rice lines, and two cytoplasmic male sterile lines (JelodarA and NematA) were screened using molecular markers. Six SSR markers were employed to identify the allelic status of the major fertility restorer genes Rf3 and Rf4.

Results and conclusion

Results and conclusion. Results of molecular testing indicated that the genotypes IR68061R, IR50, IR 68061-27-3-2-2-3R, IR 73014-59-2-2-2R, M9-P10-2-2-2-2-1, M9-P18-6-1-1-2-1, MILYANG 54, SUWEON 294, IR 9761-19-1, and IR46R possessed the Rf4 gene through three markers: RM171, RM6100, and RM228. Additionally, the presence of both fertility restorer genes Rf3 and Rf4 in the IR67924R, IR 57301-158-1R, M9-P12-5-3-2-2, M9-P15-6-2-1, and NSIC RC 352 genotypes was confirmed with all markers. These genotypes were identified as the best fertility restorers for hybrid rice breeding. Overall, the identification and evaluation of these genotypes can facilitate improved rice production and enhance food security in developing countries.

цитоплазматическая мужская стерильностьгены фертильностиселекционные программы рисатрехлинейный гибридcytoplasmic male sterilityfertility genesrice breeding programsthree-line hybridДанное исследование основано на результатах, полученных в докторской диссертации автора, ответственного за переписку. Мы благодарим руководство Сарийского университета сельскохозяйственных наук и природных ресурсов за предоставление необходимых условий для проведения данного исследования. Кроме того, aвторы благодарят рецензентов за их вклад в экспертную оценку этой работы.This research is based on the results obtained from the corresponding author’s PhD thesis. We thank the management of Sari Agricultural Sciences and Natural Resources University for providing the necessary facilities to conduct this research. Furthermore, the authors thank the reviewers for their contribution to the peer review of this work.ReferencesAfkhami Ghadi A., Babaeian Jelodar N., Bagheri N. 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The authors declare that there are no conflicts of interest present.