Rapid assessment of nutritional value indicators in Vigna unguiculata (L.) Walp. seed accessions from the VIR collection using NIR spectroscopy
https://doi.org/10.30901/2227-8834-2025-2-68-78
Abstract
Background. Cowpea (Vign a unguiculata (L.) Walp. is an important food and feed legume crop in countries with subtropical and tropical climates. Cowpea seeds have high nutritional value and contain large amounts of protein, starch, bioactive compounds, and iron, so they are often used in nutrition as a meat substitute and included in various diets. The development of calibration models for a rapid analysis of the main quality indicators in cowpea seeds based on NIR (near-infrared) spectroscopy will speed up the evaluation of accessions for nutritional value, reduce the cost of analysis, and help to preserve valuable breeding material.
Materials and methods. Seeds of 39 cowpea (V. unguiculata) accessions with different seed coat colors from the collection of the N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR) were analyzed. Quality indicators (protein, starch, and total anthocyanin content) were studied using analytical techniques accepted at VIR: the Kjeldahl method for protein content, the Evers method for starch, and spectrophotometry for total anthocyanins. Calibration models were calculated using the OPUS software developed for the MATRIX-I IR analyzer (Bruker Optics, Germany).
Results and conclusion. The use of the developed calibration models for assessing the protein, total anthocyanin and starch content in cowpea seeds makes it possible to perform their mass analysis with high precision. This will accelerate the selection of source material for breeding cultivars with high seed quality indicators and make it more accurate.
Supporting agencies: the work was carried out within the framework of the state task according to the theme plan of VIR, Project No. FGEM-2022-0002 “Identifying possibilities in the genetic diversity of leguminous crops to optimize their breeding and diversify uses in various sectors of the national economy”. The authors thank the reviewers for their contribution to the peer review of this work.
About the Authors
T. V. Shelenga
N.I. Vavilov All-Russian Institute of Plant Genetic Resources
Russian Federation
Russian Federation
Tatiana V. Shelenga, Cand. Sci. (Biology), Leading Researcher
42, 44 Bolshaya Morskaya Street, St. Petersburg 190000
A. A. Nikiforov
N.I. Vavilov All-Russian Institute of Plant Genetic Resources
Russian Federation
Russian Federation
Artur A. Nikiforov, Postgraduate Student
42, 44 Bolshaya Morskaya Street, St. Petersburg 190000
M. O. Burlyaeva
N.I. Vavilov All-Russian Institute of Plant Genetic Resources
Russian Federation
Russian Federation
Marina O. Burlyaeva, Cand. Sci. (Biology), Leading Researcher
42, 44 Bolshaya Morskaya Street, St. Petersburg 190000
I. N. Perchuk
N.I. Vavilov All-Russian Institute of Plant Genetic Resources
Russian Federation
Russian Federation
Irina N. Perchuk, Cand. Sci. (Biology), Senior Researcher
42, 44 Bolshaya Morskaya Street, St. Petersburg 190000
V. S. Popov
N.I. Vavilov All-Russian Institute of Plant Genetic Resources
Russian Federation
Russian Federation
Vitaliy S. Popov, Cand. Sci. (Engineering), Senior Researcher
42, 44 Bolshaya Morskaya Street, St. Petersburg 190000
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Review
For citations:
Shelenga T.V., Nikiforov A.A., Burlyaeva M.O., Perchuk I.N., Popov V.S. Rapid assessment of nutritional value indicators in Vigna unguiculata (L.) Walp. seed accessions from the VIR collection using NIR spectroscopy. Proceedings on applied botany, genetics and breeding. 2025;186(2):68-78. (In Russ.) https://doi.org/10.30901/2227-8834-2025-2-68-78
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