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The use of metabolomic profiling to evaluate Aegilops tauschii Coss accessions of various geographical origin from the VIR collection

https://doi.org/10.30901/2227-8834-2025-2-57-67

Abstract

Background. Aegilops tauschii Coss. is a diploid cereal species carrying the D genome of polyploid wheat, which determines the baking properties of bread wheat. A. tauschii is often used to improve old cultivars of Triticum aestivum L. through breeding, and develop new ones. The collection of the N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR) contains over 5,000 accessions of 27 Aegilops L. spp. of various ecogeographic origin, serving as a breeding resource. Identifying donors of valuable agronomic traits involves an in-depth study of the collection materials, including finding the dependence of biochemical parameters on the region of the accession’s origin. Metabolomic profiling was applied to assess the differences between A. tauschii seed accessions originating from regions with contrasting climate conditions.
Materials and methods. Two accessions of A. tauschii from the VIR collection, contrasting in their origin, served as the material for the study: k-1958 from Iran, and k-340 from Azerbaijan. Both were grown at Dagestan Experimental Station of VIR.
Results and conclusion. Statistically significant differences were found between the metabolomic profiles of A. tauschii seed accessions originating from regions with contrasting climates: subtropical semi-arid (Azerbaijan), and subtropical humid (Iran).

About the Authors

T. V. Shelenga
N.I. Vavilov All-Russian Institute of Plant Genetic Resources
Russian Federation

Tatiana V. Shelenga, Cand. Sci. (Biology), Leading Researcher 

42, 44 Bolshaya Morskaya Street, St. Petersburg 190000 



L. L. Malyshev
N.I. Vavilov All-Russian Institute of Plant Genetic Resources
Russian Federation

Leonid L. Malyshev, Cand. Sci. (Agriculture), Leading Researcher 

42, 44 Bolshaya Morskaya Street, St. Petersburg 190000 



M. Kh. Belousova
N.I. Vavilov All-Russian Institute of Plant Genetic Resources, Dagestan Experiment Station – branch of VIR, Derbentsky District
Russian Federation

Maria Kh. Belousova, Cand. Sci. (Biology), Senior Researcher 

Vavilovo Village, Derbentsky District 368612, Republic of Dagestan 



Yu. V. Ivanova
N.I. Vavilov All-Russian Institute of Plant Genetic Resources
Russian Federation

Yulia V. Ivanova, Postgraduate Student 

42, 44 Bolshaya Morskaya Street, St. Petersburg 190000 



Yu. A. Kerv
N.I. Vavilov All-Russian Institute of Plant Genetic Resources
Russian Federation

Yulia A. Kerv, Cand. Sci. (Biology), Researcher 

42, 44 Bolshaya Morskaya Street, St. Petersburg 190000 



V. S. Popov
N.I. Vavilov All-Russian Institute of Plant Genetic Resources
Russian Federation

Vitaliy S. Popov, Cand. Sci. (Engineering), Senior Researcher 

42, 44 Bolshaya Morskaya Street, St. Petersburg 190000 



V. I. Khoreva
N.I. Vavilov All-Russian Institute of Plant Genetic Resources
Russian Federation

Valentina I. Khoreva, Cand. Sci. (Biology), Leading Researcher 

42, 44 Bolshaya Morskaya Street, St. Petersburg 190000 



N. N. Chikida
N.I. Vavilov All-Russian Institute of Plant Genetic Resources
Russian Federation

Nadezhda N. Chikida, Cand. Sci. (Agriculture), Leading Researcher 

42, 44 Bolshaya Morskaya Street, St. Petersburg 190000 



References

1. Kalia B., Wilson D.L., Bowden R.L., Singh R.P., Gill B.S. Adult plant resistance to Puccinia triticina in a geographically diverse collection of Aegilops tauschii. Genetic Resources and Crop Evolution. 2017;64(5):913-926. DOI: 10.1007/s10722-016-0411-2

2. Kolesova M.A., Chikida N.N., Belousova M.Kh., Tyryshkin L.G. Effective resistance to powdery mildew in Aegilops L. accessions. Proceedings on Applied Botany, Genetics and Breeding. 2020;181(3):135-140. [in Russian] DOI: 10.30901/2227-8834-2020-3-135-140

3. Konarev V.G. Wheat proteins (Belki pshenitsy). Moscow: Kolos; 1980. [in Russian]

4. Loskutov I.G., Shelenga T.V., Konarev A.V., Khoreva V.I., Kerv Y.A., Blinova E.V. et al. Assessment of oat varieties with different levels of breeding refinement from the Vavilov Institute’s collection applying the method of metabolomic profiling. Proceedings on Applied Botany, Genetics and Breeding. 2022;183(1):104-117. DOI: 10.30901/2227-8834-2022-1-104-117

5. Majka M.M., Kwiatek M.T., Majka J., Wisniewska H. Aegilops tauschii accessions with geographically diverse origin show differences in chromosome organization and polymorphism of molecular markers linked to leaf rust and powdery mildew resistance genes. Frontiers in Plant Science. 2017;8:1149. DOI: 10.3389/fpls.2017.01149

6. Malysheva N.Yu., Shelenga T.V., Solovyeva A.E., Nagiev T.B., Kovaleva N.V., Malyshev L.L. Metabolomic approach to investigate Dactylis glomerata L. from the VIR collection. Vavilov Journal of Genetics and Breeding. 2023;27(2):111-118. DOI: 10.18699/VJGB-23-16

7. Niu Z., Chao S., Cai X., Whetten R.B., Breiland M., Cowger C. et al. Molecular and cytogenetic characterization of six wheat-Aegilops markgrafii disomic addition lines and their resistance to rusts and powdery mildew. Frontiers in Plant Science. 2018;9:1616. DOI: 10.3389/fpls.2018.01616

8. Plotnikova L.Ya., Pozherukova V.E., Knaub V.V., Lysenko N.S. Resistance of the Triticum and Aegilops species to drought and fungal diseases in Western Siberia. Vestnik of Omsk SAU. 2022;3(47):49-63. [in Russian] DOI: 10.48136/2222-0364_2022_3_49

9. Puzanskiy R.K., Shavarda A.L., Tarakhovskaya E.R., Shishova M.F. Analysis of metabolic profile of Chlamydomonas reinhardtii cultivated under autotrophic conditions. Applied Biochemistry and Microbiology. 2015;51(1):83-94. DOI: 10.1134/S0003683815010135

10. Rakszegi M., Molnár I., Darkó É., Tiwari V.K., Shewry P. Editorial: Aegilops: promising genesources to improve agronomical and quality traits of wheat. Frontiers in Plant Science. 2020;11:1060. DOI: 10.3389/fpls.2020.01060

11. Rawat N., Schoen A., Singh L., Mahlandt A., Wilson D.L., Liu S. et al. TILL-D: An Aegilops tauschii TILLING resource for wheat improvement. Frontiers in Plant Science. 2018;9:1665. DOI: 10.3389/fpls.2018.01665

12. Shelenga T.V., Malyshev L.L., Kerv Yu.A., Diubenko T.V., Konarev A.V., Horeva V.I. et al. Metabolomic approach to search for fungal resistant forms of Aegilops tauschii Coss. from the VIR collection. Vavilov Journal of Genetics and Breeding. 2020;24(3):252-258. DOI: 10.18699/VJ20.618

13. Zhang D., Zhou Y., Zhao X., Lv L., Zhang C., Li J. et al. Development and utilization of introgression lines using synthetic octaploid wheat (Aegilops tauschii × hexaploid wheat) as donor. Frontiers in Plant Science. 2018;9:1113. DOI: 10.3389/fpls.2018.01113


Review

For citations:


Shelenga T.V., Malyshev L.L., Belousova M.Kh., Ivanova Yu.V., Kerv Yu.A., Popov V.S., Khoreva V.I., Chikida N.N. The use of metabolomic profiling to evaluate Aegilops tauschii Coss accessions of various geographical origin from the VIR collection. Proceedings on applied botany, genetics and breeding. 2025;186(2):57-67. (In Russ.) https://doi.org/10.30901/2227-8834-2025-2-57-67

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ISSN 2227-8834 (Print)
ISSN 2619-0982 (Online)