Changes in the metabolomic profiles of Triticum aestivum L. under the influence of aluminum ions
Abstract
Background. Al3+ causes disruption of plant growth and development, which leads to a decrease in the yield of staple crops. Identification of aluminum tolerance markers in wheat is an urgent and promising task for prebreeding, including the development of cultivars with complex resistance to stressors.
Materials and methods. Twenty winter bread wheat accessions from the VIR collection served as the research material. From 2007 through 2019, they underwent field testing for resistance to biotic and abiotic factors of overwintering under the conditions of Northwest Russia (Pushkin). Aluminum resistance of Triticum aestivum L. accessions was studied at the early stages of plant development according to the technique modified by I. N. Kosareva. Metabolic profiles in the control and experimental groups of T. aestivum root samples were studied using nontargeted metabolomic profiling with gas chromatography coupled to mass spectrometry (Agilent 6850A, USA).
Results. The analysis showed that Al+3 stimulated the accumulation of individual free amino acids and reduced the intensity of metabolism for carbohydrates, most fatty acids, and simple phenolic compounds of the phenylpyranoid pathway. Heteroaromatic phenols, terpenes, phytosterols, oligosaccharides, monoacylglycerol, and derivatives of organic and phosphoric acids prevailed in the experimental samples of T. aestivum seedling roots, compared to the control group.
Conclusion. The results of this study will facilitate the identification of T. aestivum accessions with the most explicit protective mechanisms against Al3+ for further use in breeding programs aimed at obtaining aluminum-tolerant high-yielding cultivars of T. aestivum.
About the Authors
N. S. LysenkoRussian Federation
Natalia S. Lysenko, Senior Researcher, VIR
42, 44 Bolshaya Morskaya Street, St. Petersburg 190000, Russia
R. K. Puzanskiy
Russian Federation
Roman K. Puzanskiy, Cand. Sci. (Biology), Researcher
2 Professora Popova Street, St. Petersburg 197022, Russia
T. V. Shelenga
Russian Federation
Tatiana V. Shelenga, Cand. Sci. (Biology), Leading Researcher, VIR
42, 44 Bolshaya Morskaya Street, St. Petersburg 190000, Russia
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Lysenko N.S., Puzanskiy R.K., Shelenga T.V. Changes in the metabolomic profiles of Triticum aestivum L. under the influence of aluminum ions. Proceedings on applied botany, genetics and breeding.
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