Identification of fertility restorer genes Rf3 and Rf4 in diverse rice genotypes using SSR molecular markers
Abstract
Background. Enhancing rice yields through hybridization and the identification of fertility restorer genes is crucial for improving self-sufficiency and addressing nutritional needs. This study aims to explore the presence of these fertility restorer genes in selected rice genotypes using SSR markers.
Materials and methods. To identify the fertility restorer gene(s) in diverse rice genotypes, including local and breeding rice varieties, foreign fertility restorers, advanced mutant rice lines, and two cytoplasmic male sterile lines (JelodarA and NematA) were screened using molecular markers. Six SSR markers were employed to identify the allelic status of the major fertility restorer genes Rf3 and Rf4.
Results and conclusion. Results of molecular testing indicated that the genotypes IR68061R, IR50, IR 68061-27-3-2-2-3R, IR 73014-59-2-2-2R, M9-P10-2-2-2-2-1, M9-P18-6-1-1-2-1, MILYANG 54, SUWEON 294, IR 9761-19-1, and IR46R possessed the Rf4 gene through three markers: RM171, RM6100, and RM228. Additionally, the presence of both fertility restorer genes Rf3 and Rf4 in the IR67924R, IR 57301-158-1R, M9-P12-5-3-2-2, M9-P15-6-2-1, and NSIC RC 352 genotypes was confirmed with all markers. These genotypes were identified as the best fertility restorers for hybrid rice breeding. Overall, the identification and evaluation of these genotypes can facilitate improved rice production and enhance food security in developing countries.
About the Authors
A. Afkhami GhadiIslamic Republic of Iran
Ammar Afkhami Ghadi, PhD, Assistant Professor, Ardabil Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO); Assistant Professor, Imam Khomeini International University
12th km of Aslandoz Road, Parsabad Moghan, Ardabil Province 57451-56951, Iran; Imam Khomeini International University Blvd., Persian Gulf Ave., Qazvin 34148-96818, Iran
R. Khademian
Islamic Republic of Iran
Raheleh Khademian, PhD, Assistant Professor, Imam Khomeini International University,
Imam Khomeini International University Blvd., Persian Gulf Ave., Qazvin 34148-96818, Iran
G. A. Nematzadeh
Islamic Republic of Iran
Ghorban Ali Nematzadeh, PhD, Professor, Sari Agricultural Sciences and Natural Resources University
9th km of Farahabad Road, Sari, Mazandaran Province 48181-66996, Iran
N. Babaeian Jelodar
Islamic Republic of Iran
Nadali Babaeian Jelodar, PhD, Professor, Sari Agricultural Sciences and Natural Resources University
9th km of Farahabad Road, Sari, Mazandaran Province 48181-66996, Iran
N. Bagheri
Islamic Republic of Iran
Nadali Bagheri, PhD, Associate Professor, Sari Agricultural Sciences and Natural Resources University
9th km of Farahabad Road, Sari, Mazandaran Province 48181-66996, Iran
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For citations:
Afkhami Ghadi A., Khademian R., Nematzadeh G.A., Babaeian Jelodar N., Bagheri N. Identification of fertility restorer genes Rf3 and Rf4 in diverse rice genotypes using SSR molecular markers. Proceedings on applied botany, genetics and breeding.
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