Preview

Proceedings on applied botany, genetics and breeding

Advanced search

Development of the potato cryocollection preserved in the VIR cryobank

https://doi.org/10.30901/2227-8834-2023-3-9-20

Abstract

Background. The article presents the results of the studies carried out within the framework of a complex program for the long-term conservation of genotyped accessions of advanced potato cultivars in the cryobank of the N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR). These accessions are genetically identical to the nomenclature standards of the corresponding cultivars.

Materials and methods. The research material included 24 accessions from the in vitro collection of VIR, corresponding to the nomenclature standards of Russian potato cultivars developed in different breeding centers of the Russian Federation. Cryopreservation was carried out using the droplet vitrification method, several stages of which were modified in the Department of Biotechnology of VIR.

Results and discussion. The frequency of post-cryogenic regeneration for the analyzed 24 cultivars in control variants with short-term immersion of explants in liquid nitrogen varied from 20 to 47.3%, and in 9 accessions it exceeded 39% – the current level of reliable long-term preservation of samples in cryobanks. The data of the statistical analysis failed to reveal a significant effect of the genotype or the calendar dates of different experiment replications on the regeneration ability. These 24 accessions were added to the potato cryocollection stored in the VIR cryobank. An analysis of the composition and structure of VIR’s potato cryocollection, which currently includes 140 accessions, is presented.

About the Authors

O. S. Efremova
N.I. Vavilov All-Russian Institute of Plant Genetic Resources
Russian Federation

Olga S. Efremova, Cand. Sci. (Agriculture), Researcher

42, 44 Bolshaya Morskaya Street, St. Petersburg 190000



N. N. Volkova
N.I. Vavilov All-Russian Institute of Plant Genetic Resources
Russian Federation

Natalia N. Volkova, Leading Specialist

42, 44 Bolshaya Morskaya Street, St. Petersburg 190000



D. A. Rybakov
N.I. Vavilov All-Russian Institute of Plant Genetic Resources
Russian Federation

Daniil А. Rybakov, Associate Researcher

42, 44 Bolshaya Morskaya Street, St. Petersburg 190000



O. V. Lisitsyna
N.I. Vavilov All-Russian Institute of Plant Genetic Resources
Russian Federation

Olga V. Lisitsyna, Leading Specialist

42, 44 Bolshaya Morskaya Street, St. Petersburg 190000



P. V. Ozerski
N.I. Vavilov All-Russian Institute of Plant Genetic Resources
Russian Federation

Pavel V. Ozerski, Cand. Sci. (Biology), Senior Researcher

42, 44 Bolshaya Morskaya Street, St. Petersburg 190000



Т. A. Gavrilenko
N.I. Vavilov All-Russian Institute of Plant Genetic Resources
Russian Federation

Tatjana A. Gavrilenko, Dr. Sci. (Biology), Chief Researcher, Head of a Department

42, 44 Bolshaya Morskaya Street, St. Petersburg 190000



References

1. Bamberg J.B., Martin M.W, Abad J, Jenderek M.M., Tanner J.D., Donnelly D. et al. In vitro technology at the US Potato Genebank. In Vitro Cellular and Developmental Biology – Plant. 2016;52(3):213-225. DOI: 10.1007/s11627-016-9753-x

2. CIP Genebank: [website]. Available from: https://cipotato.org [accessed Sept. 20, 2022].

3. Dunaeva S.E., Pendinen G.I., Antonova O.Yu., Shvachko N.A., Ukhatova Yu.V., Shuvalova L.E., Volkova N.N., Gavrilenko T.A. Preservation of vegetatively propagated crops in vitro and cryo collections: methodological guidelines (Sokhraneniye vegetativno razmnozhayemykh kultur v in-vitro- i kriokollektsiyakh: metodicheskiye ukazaniya). T.A. Gavrilenko (ed.). 2nd ed. St. Petersburg: VIR; 2017. [in Russian]

4. Dussert S., Engelmann F., Noirot M. Development of probabilistic tools to assist in the establishment and management of cryopreserved plant germplasm collections. CryoLetters. 2003;24(3):149-160.

5. Efremova O.S., Volkova N.N., Gavrilenko T.A. Long-term preservation of modern Russian potato cultivars in the VIR cryobank. Plant Biotechnology and Breeding. 2020;3(3):68-76. [in Russian] DOI: 10.30901/2658-6266-2020-3-o1

6. Engelmann F., Takagi H. (eds). Cryopreservation of tropical plant germplasm. Current research progress and application. Tsukuba: JIRCAS; Roma: IPGRI; 2000. Available from: https://horizon.documentation.ird.fr/exl-doc/pleins_textes/2021-08/010023387.pdf [accessed Sept. 12, 2022].

7. Fomina N.A., Antonova O.Yu., Chukhina I.G., Gimaeva E.A., Stashevski Z., Gavrilenko T.A. Nomenclatural standards and genetic passports of potato cultivars bred by the Tatar Research Institute of Agriculture “Kazan Scientific Center of the Russian Academy of Sciences”. Plant Biotechnology and Breeding. 2020;3(3):55-67. [in Russian] DOI: 10.30901/2658-6266-2020-3-o4

8. Forsline P.L., McFerson J.R., Lamboy W.F., Towill L.E. Development of base and active collections of Malus germplasm with cryopreserved dormant buds. Acta Horticulturae. 1999;484:75-78. DOI: 10.17660/ActaHortic.1998.484.8

9. Gavrilenko T., Antonova O., Ovchinnikova A., Novikova L., Krylova E., Mironenko N. et al. A microsatellite and morphological assessment of the Russian National Potato Collection. Genetic Resources and Crop Evolution. 2010;57(8):1151-1164. DOI: 10.1007/s10722-010-9554-8

10. Gavrilenko T., Antonova O., Shuvalova A., Krylova E., Alpatyeva N., Spooner D.M. et al. Genetic diversity and origin of cultivated potatoes based on plastid microsatellite polymorphism. Genetic Resources and Crop Evolution. 2013;60(7):1997-2015. DOI: 10.1007/s10722-013-9968-1

11. Gavrilenko T.A., Chukhina I.G. Nomenclatural standards of modern Russian potato cultivars preserved at the VIR herbarium (WIR): A new approach to cultivar genepool registration in a genebank. Plant Biotechnology and Breeding. 2020;3(3):6-17. [in Russian] DOI: 10.30901/2658-6266-2020-3-o2

12. Gavrilenko T.A., Shvachko N.A., Volkova N.N., Ukhatova Yu.V. A modified droplet vitrification method for cryopreservation of shoot tips from in vitro potato plants. Vavilov Journal of Genetics and Breeding. 2019;23(4):422-429. [in Russian] DOI: 10.18699/VJ19.505

13. Genesys PGR: [website]. Available from: https://www.genesys-pgr.org [accessed Sept. 20, 2022].

14. Hammer Ø., Harper D.A.T., Ryan P.D. PAST: paleontological statistics software. Package for education and data analysis. Palaeontologia Electronica. 2001;4(1):4. Available from: http://palaeo-electronica.org/2001_1/past/past.pdf [accessed Sept. 06, 2022].

15. Hirai D. Gelled droplet vitrification improves recovery of cryopreserved potato germplasm. CryoLetters. 2011;32(4):287-296.

16. Kaczmarczyk A., Rokka V.M., Keller E.R.J. Potato shoot tip cryopreservation. A review. Potato Research. 2010;54(1):45-79. DOI: 10.1007/s11540-010-9169-7

17. Klimenko N.S., Gavrilenko T.A., Chukhina I.G., Gadzhiev N.M., Evdokimova Z.Z., Lebedeva V.A. Nomenclatural standards and genetic passports of potato cultivars bred at the Leningrad Research Institute for Agriculture “Belogorka”. Plant Biotechnology and Breeding. 2020;3(3):18-54. [in Russian] DOI: 10.30901/2658-6266-2020-3-o3

18. Kobzar A.I. Applied mathematical statistics (Prikladnaya matematicheskaya statistika). Moscow: Fizmatlit; 2006. [in Russian]

19. Köpnick C., Grübe M., Stock J., Senula A., Mock H.P., Nagel M. Changes of soluble sugars and ATP content during DMSO droplet freezing and PVS3 droplet vitrification of potato shoot tips. Cryobiology. 2018;85:79-86. DOI: 10.1016/j.cryobiol.2018.09.005

20. Murashige T., Skoog F.A. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum. 1962;15(3):473-497. DOI: 10.1111/j.1399-3054.1962.tb08052.x

21. Muthoni J., Shimelis H., Melis R. Long-term conservation of potato genetic resources: Methods and status of conservation. Australian Journal of Crop Science. 2019;13(5):717-725. DOI: 10.21475/ajcs.19.13.05.p1400

22. Nagel M., Dulloo M.E., Bissessur P., Gavrilenko T., Bamberg J., Ellis D., Giovannini P. A global strategy for the conservation of potato. Bonn: Global Crop Diversity Trust; 2022. DOI: 10.5447/ipk/2022/29

23. Niino T., Arizaga M.V. Cryopreservation for preservation of potato genetic resources. Breeding Science. 2015;65(1):41-52. DOI: 10.1270/jsbbs.65.41

24. Panis B., Nagel M., Van den Houwe I. Challenges and prospects for the conservation of crop genetic resources in field genebanks, in in vitro collections and/or in liquid nitrogen. Plants. 2020;9(12):1634. DOI: 10.3390/plants9121634

25. Panis B., Piette B., Swennen R. Droplet vitrification of apical meristems: a cryopreservation protocol applicable to all Musaceae. Plant Science. 2005;168(1):45-55. DOI: 10.1016/j.plantsci.2004.07.022

26. Panis B., Van den Houwe I., Swennen R., Rhee J., Roux N. Securing plant genetic resources for perpetuity through cryopreservation. Indian Journal of Plant Genetic Resources. 2016;29(3):300-302. DOI: 10.5958/0976-1926.2016.00051.6

27. Panta A., Panis B., Ynouye C., Criel B., Swennen R., Roca W. 78. Improvement of potato cryopreservation for the longterm conservation of Andean landraces at the International Potato Center (CIP). Cryobiology. 2006;53(3):401. DOI: 10.17660/ActaHortic.2011.908.45

28. Rybakov D.A., Cheremisin A.I., Antonova O.Yu., Chukhina I.G., Gavrilenko T.A. Nomenclatural standards and genetic passports of potato cultivars bred by the Omsk Agrarian Research Center. Plant Biotechnology and Breeding. 2022;5(4):6-23. [in Russian] DOI: 10.30901/2658-6266-2022-4-o4

29. Sakai A. Potentially valuable cryogenic procedures for cryopreservation of cultured plant meristems. In: M.K. Razdan, E.C. Cocking (eds). Conservation of Plant Genetic Resources in Vitro. Volume 1: General Aspects. Enfield, NC: Science Publishers Inc.; 1997. p.53-66.

30. Sakai A., Kobayashi S., Oiyama I. Cryopreservation of nucellar cells of navel orange (Citrus sinensis Osb. var. brasiliensis Tanaka) by vitrification. Plant Cell Reports. 1990;9(1):30-33. DOI: 10.1007/bf00232130

31. Shvachko N.A. Genetic diversity of potato cultivars in the VIR collection identified by the SSR analysis (Geneticheskoye raznoobraziye selektsionnykh sortov kartofelya kollektsii VIR, vyyavlennoye SSR analizom) [dissertation]. St. Petersburg: VIR; 2012. [in Russian] URL: http://vir.nw.ru/biot/pdf/autoreferat_shvachko_2012.pdf [дата обращения: 05.09.2022].

32. Stock J., Mock H.P., Senula A., Nagel M. Arabidopsis – a model to elucidate complex stress response mechanism during cryopreservation. Acta Horticulturae. 2019;1234:85-96. DOI: 10.17660/ActaHortic.2019.1234.11

33. Towill L.E. Improved survival after cryogenic exposure of shoot tips derived from in vitro plantlet cultures of potato. Cryobiology. 1983;20(5):567-573. DOI: 10.1016/0011-2240(83)90045-7

34. Ukhatova Yu.V. Improving the methods of cryopreservation and recovery from viral diseases of vegetatively propagated crop accessions (Sovershenstvovaniye metodov kriokonservatsii i ozdorovleniya ot virusnykh bolezney obraztsov vegetativno razmnozhayemykh kultur) [dissertation]. St. Petersburg: VIR; 2017. [in Russian] URL: http://vir.nw.ru/desert/yhatova/diss_ukhatova.pdf [дата обращения: 06.09.2022].

35. Ukhatova Yu.V., Gavrilenko T.A. Cryoconservation methods for vegetatively propagated crops. Plant Biotechnology and Breeding. 2018;1(1):52-63. [in Russian] DOI: 10.30901/2658-6266-2018-1-52-63

36. VIR Databases: [website]. [in Russian] URL: http://db.vir.nw.ru/virdb/maindb [дата обращения: 20.09.2022].

37. Vollmer R., Villagaray R., Cárdenas J., Castro M., Chávez O., Anglin N.L. et al. A large-scale viability assessment of the potato cryobank at the International Potato Center (CIP). In Vitro Cellular and Developmental Biology – Plant. 2017;53(4):309-317. DOI: 10.1007/s11627-0179846-1

38. Vollmer R., Villagaray R., Castro M., Cárdenas J., Pineda S., Espirilla J. et al. The world’s largest potato cryobank at the International Potato Center (CIP) – Status quo, protocol improvement through large-scale experiments and long-term viability monitoring. Frontiers in Plant Science. 2022;13:1059817. DOI: 10.3389/fpls.2022.1059817

39. Vollmer R., Villagaray R., Egusquiza V., Espirilla J., García M., Torres A. et al. The potato cryobank at the International Potato Center (CIP): A model for long-term conservation of clonal plant genetic resources collections of the future. CryoLetters. 2016;37(5):318-329.


Supplementary files

1. Приложение_Supplementary file
Subject Structure and composition of the VIR cryocollection
Type Исследовательские инструменты
Download (567KB)    
Indexing metadata ▾

Review

For citations:


Efremova O.S., Volkova N.N., Rybakov D.A., Lisitsyna O.V., Ozerski P.V., Gavrilenko Т.A. Development of the potato cryocollection preserved in the VIR cryobank. Proceedings on applied botany, genetics and breeding. 2023;184(3):9-20. (In Russ.) https://doi.org/10.30901/2227-8834-2023-3-9-20

Views: 554


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.


ISSN 2227-8834 (Print)
ISSN 2619-0982 (Online)