Studying the effect of the OsGATA rice transcription factor on salt stress tolerance in wheat
https://doi.org/10.30901/2227-8834-2022-3-9-16
Abstract
This study shows the possibility of using the OsGATA rice transcription factor in transgenic lines of high-yielding wheat cultivars to increase their tolerance to salinity, which was confirmed using physiological and biochemical methods according to standard protocols. Wheat plants were grown in an artificial climate under optimal growing conditions. Genetic transformation methods were used to introduce the GATA gene into the genome of the used wheat genotypes. Transgenic lines were selected on selective media under in vitro conditions.
The results of the experimental work showed that the expression of the GATA gene under salt stress may be responsible for the increased compartmentalization of Na+ in the vacuole, which provides improved salt tolerance. As a result of the experiment, collections of T1 transgenic wheat lines from cvs. ‘Zlata’, ‘Emir’ and ‘Agata’ expressing the GATA gene were obtained and studied for salt tolerance. Lines Zl.01, Zl.02, Zl.03 and Ag.02 were selected with PCR. Under NaCl salinity conditions, some of the transgenic lines showed a statistically significant increase in salinity resistance. The results of the study laid the foundation for studying GATA genes in wheat and for producing salinity-tolerant lines without growth defects or reduced productivity.
Supporting agencies: The study was funded by the Russian Foundation for Basic Research in the framework of Research Project No. 19-316-90063. The authors thank the reviewers for their contribution to the peer review of this work.
About the Authors
A. A. Verbitskaia
Koltzov Institute of Developmental Biology, Russian Academy of Sciences
Russian Federation
Russian Federation
Anastasiia A. Verbitskaia - Associate Researcher, Koltzov Institute of Developmental Biology, Russian Academy of Sciences.
26 Vavilova St., Moscow 119334.
A. S. Egorova
Vavilov Institute of General Genetics, Russian Academy of Sciences
Russian Federation
Russian Federation
Anna S. Egorova - Associate Researcher, Vavilov Institute of General Genetics, Russian Academy of Sciences.
3 Gubkina St., Moscow 119991.
E. A. Tsarkova
Vavilov Institute of General Genetics, Russian Academy of Sciences
Russian Federation
Russian Federation
Elena A. Tsarkova - Associate Researcher, Vavilov Institute of General Genetics, Russian Academy of Sciences.
3 Gubkina St., Moscow 119991.
A. K. Gaponenko
Vavilov Institute of General Genetics, Russian Academy of Sciences
Russian Federation
Russian Federation
Alexander K. Gaponenko - Dr. Sci. (Biology), Chief Researcher, Vavilov Institute of General Genetics, Russian Academy of Sciences.
3 Gubkina St., Moscow 119991.
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Review
For citations:
Verbitskaia A.A., Egorova A.S., Tsarkova E.A., Gaponenko A.K. Studying the effect of the OsGATA rice transcription factor on salt stress tolerance in wheat. Proceedings on applied botany, genetics and breeding. 2022;183(3):9-16. (In Russ.) https://doi.org/10.30901/2227-8834-2022-3-9-16
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