LOCAL BARLEY ACCESSIONS FROM ETHIOPIA RESISTANT TO POWDERY MILDEW
https://doi.org/10.30901/2227-8834-2016-4-70-78
Abstract
The mlo11 allele associated with the loss of function of the Mlo locus and controlling mildew resistance in the majority of modern barley cultivars was identified in Ethiopian barley landraces. Twenty-seven accessions from Ethiopia were studied during laboratory experiments which resulted in identifying k-5448 as an accession resistant to powdery mildew. Three accessions (k-8555, k-8682 k-17554) were heterogeneous by the studied trait. With the help of molecular markers we showed that the resistant components of the selected forms are protected by the mlo11 gene.
About the Authors
N. V. Alpatieva
Federal Research Center the N.I.Vavilov All-Russian Institute of Plant Genetic Resources (VIR)
Russian Federation
Russian Federation
R. A. Abdullaev
Federal Research Center the N.I.Vavilov All-Russian Institute of Plant Genetic Resources (VIR)
Russian Federation
Russian Federation
I. N. Anisimova
Federal Research Center the N.I.Vavilov All-Russian Institute of Plant Genetic Resources (VIR)
Russian Federation
Russian Federation
N. K. Gubareva
Federal Research Center the N.I.Vavilov All-Russian Institute of Plant Genetic Resources (VIR)
Russian Federation
Russian Federation
O. N. Kovaleva
Federal Research Center the N.I.Vavilov All-Russian Institute of Plant Genetic Resources (VIR)
Russian Federation
Russian Federation
E. E. Radchenko
Federal Research Center the N.I.Vavilov All-Russian Institute of Plant Genetic Resources (VIR)
Russian Federation
Russian Federation
References
1. Анисимова И. Н., Алпатьева Н. В., Тимофеева Г. И. Скрининг генетических ресурсов растений с использованием ДНК-маркеров: основные принципы, выделение ДНК, постановка ПЦР, электрофорез в агарозном геле: Методические указания ВИР / под ред. Е. Е. Радченко. СПб: ВИР. 2010. 30 с.
2. Basic Local Alignment Search Tool. http://blast.ncbi.nlm.nih.gov/Blast.cgi
3. Buschges R., Hollricher K., Panstruga R., Simons G., Wolter M., Frijters A., Van Daelen R., Van der Lee T., Diergaarde P., Groenendijk J., Topsch S., Vos P., Salamini F., Schulze-Lefert P. The barley Mlo gene: a novel control element of plant pathogen resistance // Cell, 1997, vol. 88, no. 5, pp. 695-705. DOI: http:dx.doi.org/10.1016/S0092-8674(00)81912-1
4. Dorokhov D. B., Klocke E. A. Rapid and economic technique for RAPD analysis of plant genomes // Rus. J. Genet., 1997, vol. 33, no. 4, pp. 358-365
5. Dreiseitl A. Frequency of powdery mildew resistances in spring barley cultivars in Czech variety trials // Plant Protection Sci., 2012, vol. 48, no. 1, pp. 17-20
6. Идентификация сортов и регистрация генофонда культурных растений по белкам семян / под ред. акад. РАСХН В. Г. Конарева. СПб.: ВИР, 2000. 186 с.
7. Jorgensen J. H. Discovery, characterization and exploitation of Mlo powdery mildew resistance in barley // Euphytica, 1992, vol. 63, no. 1-2, pp. 141-152. DOI: 10.1007/BF00023919
8. Кривченко В. И., Лебедева Т. В., Пеуша Х. О. Мучнистая роса // Изучение генетических ресурсов зерновых культур по устойчивости к вредным организмам. Методическое пособие. М.: Россельхозакадемия, 2008. С. 86-105
9. Piffanelli P., Ramsay L., Waugh R., Benabdelmouna A., D’Hont A., Hollricher K., Jorgensen J. H., Schulze-Lefert P., Panstruga R. A barley cultivation-associated polymorphism conveys resistance to powdery mildew // Nature, 2004, vol 430, no. 7002, pp. 887891. D0I:10.1038/nature02781
10. Reinstadler A., Muller J., Jerzy H., Czembor J. H., Piffanelli P., Panstruga R. Novel induced mlo mutant alleles in combination with site-directed mutagenesis reveal functionally important domains in the heptahelical barley Mlo protein // BMC Plant Biology, 2010, vol. 10, art. no. 31. DOI: 10.1186/1471-2229-10-31
11. Weibull J., Walther U., Sato K., Habekufi A., Kopahnke D., Proeseler G. Diversity in resistance to biotic stresses // Diversity in Barley (Hordeum vulgare). Elsevier, 2003, pp. 143-178
Review
For citations:
Alpatieva N.V., Abdullaev R.A., Anisimova I.N., Gubareva N.K., Kovaleva O.N., Radchenko E.E. LOCAL BARLEY ACCESSIONS FROM ETHIOPIA RESISTANT TO POWDERY MILDEW. Proceedings on applied botany, genetics and breeding. 2016;177(4):70-78. (In Russ.) https://doi.org/10.30901/2227-8834-2016-4-70-78
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