Phytosanitary monitoring of the narrow-leaved lupine collection of VIR in the northwest of Russia

Background. Narrow-leaved lupine (Lupinus angustifolius L.) is an important high-protein forage and green manure crop, also promising for food use. This is a plastic species produced under various ecogeographic conditions and the only cultivated species of lupine adapted to high northern latitudes – up to 60° NL. Gradual expansion of lupine production areas led to the accumulation of pathogens, damage to lupine crops by harmful organisms, and significant harvest losses. Breeding and cultivating resistant varieties is the most profitable and environmentally sound way to control diseases and pests. Studying the species composition of pathogens and searching for source material are the necessary stages of plant breeding for immunity.

Materials and methods. A set of 101 accessions of L. angustifolius from the VIR collection were studied in the northwest of the Russian Federation (Pushkin, St. Petersburg) in 2019, and 62 accessions from the same set in 2020. Mycological examination of the affected leaves was carried out on potato sucrose agar medium under laboratory conditions. The degree of damage to plants by diseases and infestation by pests was assessed during the period of flowering, fruit formation and ripening using point scales.

Results and conclusions. A wide range of harmful organisms were identified for their negative impact on the growth and development of narrow-leaved lupine plants. The dominant pest was the lupine aphid (Macrosiphum albifrons Essig), discovered in Russia for the first time. A whole set of fungal pathogens was identified, among which the most common and harmful were the causative agents of Fusarium, brown leaf spot, powdery mildew, gray mold, and Sclerotinia stem rot. Narrow-leaved lupine accessions with weak degrees of pathogenic damage were selected.

1. Amselem J., Cuomo C.A., van Kan J.A.L., Viaud M., Benito E.P., Couloux A. et al. Genomic analysis of the necrotrophic fungal pathogens Sclerotinia sclerotiorum and Botrytis cinerea. PLoS Genetics. 2011;7(8):e1002230. DOI: 10.1371/journal.pgen.1002230

2. Аrtyukhov А.I. Lupin species adaptation to agrolandscape of Russia. Legumes and Groat Crops. 2015;1(13):60-67. [in Russian]

3. Аrtyukhov А.I., Podobedov A.V. Lupine is the important part of strategy for Russia’s self-provision with complementary protein. Fodder Production. 2012;(5):3-4. [in Russian]

4. Boland G.J., Hall R. Index of plant hosts of Sclerotinia sclerotiorum. Canadian Journal of Plant Pathology. 1994;16(2):93-108. DOI: 10.1080/07060669409500766

5. Carter C.I., Fourt D.F., Bartlett P.W. The lupin aphid’s arrival and consequences. Antenna. 1984;(8):129-132.

6. Davidson J.A., Pande S., Bretag T.W., Lindbeck K.D., KrishnaKishore G. Biology and management of Botrytis spp. in legume crops. In: Y. Elad, B. Williamson, P. Tudzynski, N. Delen (eds). Botrytis: Biology, Pathology and Control. Dordrecht: Springer; 2007. p.295-318. DOI: 10.1007/9781-4020-2626-3_16

7. Debelyj G.A. Leguminous crops in the world and in the Russian Federation. Legumes and Groat Crops. 2012;2(2):31-35. [in Russian]

8. Elad Y., Pertot I., Cotes Prado A.M., Stewart A. Plant hosts of Botrytis spp. In: S. Fillinger, Y. Elad (eds). Botrytis – the Fungus, the Pathogen and its Management in Agricultural Systems. Cham: Springer; 2016. p.413-486. DOI: 10.1007/978-3-319-23371-0_20

9. Favret C. Aphid Species File. Version 5.0/5.0. 2021. Available from: http://Aphid.SpeciesFile.org [accessed Feb. 27, 2021].

10. Ferguson A.W. Pests and plant injury on lupins in the south of England. Crop Protection. 1994;13(3):201-210. DOI: 10.1016/0261-2194(94)90079-5

11. Gresta F., Wink M., Prins U., Abberton M., Capraro J., Scarafoni A. et al. Lupins in European cropping systems. In: D. Murphy-Bokern, F. Stoddard, C. Watson (eds). Legumes in Cropping Systems. Wallingford: CABI Publishing; 2017. p.88-108. DOI: 10.1079/9781780644981.0088

12. Hinz B. Versuche zur Schadensbewertung der Lupinenblattlaus (Macrosiphum albifrons Essig) an Kulturlupinen. Journal of Applied Entomology. 1992;113(1-5):214-216. [in German] DOI: 10.1111/j.1439-0418.1992.tb00656.x

13. Holman J. Host Plant Catalog of Aphids: Palearctic Region. Dordrecht: Springer; 2009. DOI: 10.1007/978-1-40208286-3

14. Jones R.A.C., McLean G.D. Virus diseases of lupins. Annals of Applied Biology. 1989;114(3):609-637. DOI: 10.1111/j.17447348.1989.tb03376.x

15. Jones R.A.C., Pearce R.M., Prince R.T., Coutts B.A. Natural resistance to Alfalfa mosaic virus in different lupin species. Australasian Plant Pathology. 2008;37(2):112-116. DOI: 10.1071/AP07092

16. Khokhryakov M.K., Dobrozrakova T.L., Stepanov K.M., Letova M.F. Key to plant diseases (Opredelitel bolezney rasteniy). 3rd ed. St. Petersburg: Lan; 2003. [in Russian]

17. Kordan B., Gabryś B., Dancewicz K., Lahuta L.B., PiotrowiczCieślak A., Rowińska E. European yellow lupine, Lupinus luteus, and narrow-leaf lupine, Lupinus angustifolius, as hosts for the pea aphid, Acyrthosiphon pisum. Entomologia Experimentalis et Applicata. 2008;128(1):139-146. DOI: 10.1111/j.1570-7458.2008.00702.x

18. Kurlovich B.S., Golovchenko V.I. Source material for breeding of intensive-type selection lupine cultivars. (Iskhodny material dlya selektsii sortov lyupina intensivnogo tipa). Scientific and Technical Bulletin of the N.I. Vavilov All-Union Research Institute of Plant Industry. 1989;(193):25-28. [in Russian]

19. Kurlovich B.S., Kartuzova L.T., Korneychuk N.S., Kiselev I.I., Na za rova N.S., Pilipenko S.I. Catalogue of the VIR global collection. Issue 537. Lupine (Evaluation of accessions for resistance to Fusarium on infectious backgrounds). Leningrad: VIR; 1990. [in Russian]

20. Luckett D.J., Cowley R.B., Richards M.F., Roberts D.M. Breeding Lupinus albus for resistance to the root pathogen Pleiochaeta setosa. European Journal of Plant Pathology. 2009;125(1):131-141. DOI: 10.1007/s10658-009-9465-8

21. Pimokhova L.I., Yagovenko G.L., Tsarapneva Zh.V., Khara borkina N.I. A new fungicide for narrow-leafed lupin crops protection. Legumes and Groat Crops. 2020a;3(35):134-139. DOI: 10.24411/2309-348X-202011196

22. Pimokhova L.I., Yagovenko G.L., Tsarapneva Zh.V., Misni ko va N.V. Development of Sclerotinia in narrowleaf (Lupinus angustifolius L.) and white (Lupinus albus L.) lupin single and mixed crops under different weather conditions in Bryansk Region. Agricultural Biology. 2020b;55(6):1257-1267. [in Russian] DOI: 10.15389/agrobiology.2020.6.1257rus

23. Radchenko Е.Е., Abdullaev R.A., Alpatieva N.V., Putina O.V., Gasich E.L. Alternaria leaf blight of clusterbean. Vavilov Journal of Genetics and Breeding. 2019;23(6):11-19. [in Russian] DOI: 10.18699/VJ19.536

24. Rutskaya V.I. Cultivated lupine species and their main pests and diseases. Adaptive Fodder Production. 2018;(4):73-82. [in Russian]

25. Shapkina Yu.S., Strojkov Yu.M., Tsygutkin A.S., Medvedeva N.V., Veselovskaya K.N., Tyutyunov S.I. et al. Phytosa nitary status of white lupine crops in the north east and south west of the Central Black Earth Belt. Achievements of Science and Technology of AIC. 2011;(9):29-31. [in Russian]

26. State Register for Selection Achievements Admitted for Usage (National List). Vol. 1 “Plant varieties” (official publication). Moscow; Rosinformagrotekh; 2020. [in Russian] URL: https://gossortrf.ru/wpcontent/uploads/2020/03/FIN_reestr_dop_12_03_2020.pdf [дата обращения: 11.03.2021].

27. Sweetingham M. Coping with brown spot and root rots of lupins. Journal of the Department of Agriculture, Western Australia. Series 4. 1990;31(1):5-13.

28. Tomczak A., Zielińska-Dawidziak M., Piasecka-Kwiatkowska D., Lart-Szczapa E. Blue lupine seeds protein content and amino acids composition. Plant, Soil and Environment. 2018;64(4):147-155. DOI: 10.17221/690/2017-PSE

29. Vishnyakova M.A., Kushnareva A.V., Shelenga T.V., Egorova G.P. Alkaloids of narrow-leafed lupine as a factor determining alternative ways of the crop’s utilization and breeding. Vavilov Journal of Genetics and Breeding. 2020;24(6):625-635. DOI: 10.18699/VJ20.656

30. Vishnyakova M.A., Seferova I.V., Buravtseva T.V., Burlyaeva M.O., Semenova E.V., Filipenko G.I., Aleksandrova T.G., Egorova G.P., Yankov I.I., Bu lyn tsev S.V.,Gerasimova T.V., Drugova E.V. VIR global collection of grain legume crop genetic resources: replenishment, conservation and studying: (methodological guidelines). 2nd ed. M.A. Vishnyakova (ed.). St. Petersburg: VIR; 2018. [in Russian] DOI: 10.30901/978-5-905954-79-5

31. Yakusheva A.S., Solovyanova N.N.. Evaluation of lupine for resistance to anthracnose. Guidelines (Otsenka lyupina na ustoychivost k antraknozu. Metodicheskiye rekomendatsii). Bryansk: All-Russian Lupine Research Institute; 2001. [in Russian]

32. Zhorov D.G., Sinchuk O.V., Buga S.V. Lupine aphid (Macrosiphum albifrons) is a dangerous pest new for Belarus and the carrier of viral diseases (Lyupinovaya tlya (Macrosiphum albifrons) – novy dlya Belarusi opasny vreditel i perenoschik virusnykh zabolevaniy). Agriculture and Plant Protection. 2017;2(111):26-28. [in Russian]