vir-nwТруды по прикладной ботанике, генетике и селекцииProceedings on applied botany, genetics and breeding2227-88342619-0982N.I. Vavilov All-Russian Institute of Plant Genetic Resources10.30901/2227-8834-2021-2-72-80vir-nw-956Research ArticleГЕНЕТИКА КУЛЬТУРНЫХ РАСТЕНИЙ И ИХ ДИКИХ РОДИЧЕЙGENETICS OF CULTIVATED PLANTS AND THEIR WILD RELATIVESГенетическое разнообразие дикорастущих видов и сортов земляники по гену FanAAMT ароматического комплекса плодовGenetic diversity in wild species and cultivars of strawberry for the FanAAMT gene controlling fruit flavor volatileshttps://orcid.org/0000-0001-9770-8731ЛыжинА. С.LyzhinA. S.

393760, Тамбовская обл., г. Мичуринск, ул. Мичурина, 30; к.с.-х.н.

30 Michurina St., Michurinsk, Tambov Prov. 393760

Ranenburzhetc@yandex.ruhttps://orcid.org/0000-0003-1626-840XЛукъянчукИ. В.Luk’yanchukI. V.

393760, Тамбовская обл., г. Мичуринск, ул. Мичурина, 30; к.с.-х.н.

30 Michurina St., Michurinsk, Tambov Prov. 393760

irina.lk2011@yandex.ruФедеральный научный центр имени И.В. МичуринаРоссияI.V. Michurin Federal Science CenterRussian Federation20212906202118227280Copyright © Лыжин А.С., Лукъянчук И.В., 20212021Лыжин А.С., Лукъянчук И.В.Lyzhin A.S., Luk’yanchuk I.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://elpub.vir.nw.ru/jour/article/view/956

Актуальность. Важной потребительской характеристикой плодов земляники является их аромат. Значительный вклад в формирование аромата плодов земляники вносит метилантранилат. Содержание метилантранилата в плодах земляники контролируется геном FanAAMT (метилтрансфераза антраниловой кислоты). Идентификация генетических детерминант данного признака позволяет вести целенаправленный отбор перспективных форм на основе диагностических ДНК-маркеров. Целью настоящего исследования являлось изучение аллельного разнообразия гена аромата плодов FanAAMT у дикорастущих видов и сортов земляники для выявления перспективных генотипов.Материалы и методы. Объектами исследования являлись дикорастущие виды рода Fragaria L., сорта земклуники (F. × anaschata Kantor) и сорта земляники садовой (F. × ananassa Duch.), интродуцированные из различных эколого-географических регионов произрастания. Для идентификации гена FanAAMT использовали маркер FanAAMT.Результаты и выводы. В анализируемой коллекции генотипов земляники ген FanAAMT идентифицирован у 36,4% форм, в том числе – у дикорастущих видов F. moschata Duch., F. vesca L., F. virginiana subsp. platypetala (Rydb.) Staudt и сорта земклуники ‘Купчиха’. Среди проанализированных сортов земляники садовой ген FanAAMT выявлен у 31,6% образцов, при этом среди отечественных сортов ген FanAAMT идентифицирован у 27,3% форм, среди зарубежных – у 37,5% форм. Перспективными источниками высокого содержания метилантранилата в плодах являются дикорастущие виды F. vesca, F. moschata и F. virginiana subsp. platypetala, сорт земклуники ‘Купчиха’, а также сорта земляники садовой отечественной (‘Былинная’, ‘Зенит’, ‘Ласточка’, ‘Незнакомка’, ‘Фейерверк’, ‘Царскосельская’) и зарубежной (‘Karmen’, ‘Ostara’, ‘Samson’, ‘Symphony’, ‘Troubadour’, ‘Vima Tarda’) селекции, у которых идентифицирован функциональный аллель гена FanAAMT.  У остальных изученных генотипов земляники маркер FanAAMT отсутствует, что, предположительно, свидетельствует об отсутствии гена FanAAMT.

Background. An important consumer trait of strawberry fruits is their aroma. Methyl anthranilate makes a considerable contribution to the fruit flavor. The methyl anthranilate content in strawberry fruits is controlled by the FanAAMT (аnthranilic acid methyltransferase) gene. Identification of genetic determinants for this trait facilitates targeted selection of promising forms based on diagnostic DNA markers. The purpose of this study was to study the allelic diversity of the FanAAMT fruit flavor gene in wild strawberry species and strawberry cultivars for identification of promising genotypes.Materials and methods. The objects of this study were wild species of Fragaria L. as well as F. × anaschata Kantor. and F. × ananassa Duch. cultivars of different ecogeographic origin. The FanAAMT gene was identified with the dominant marker FanAAMT.Results and conclusion. In the analyzed collection of strawberry genotypes, the FanAAMT gene was identified in 36.4% of the forms, including the wild species F. vesca L., F. moschata Duch. and F. virginiana subsp. platypetala (Rydb.) Staudt, and cv. ‘Kupchikha’ (F. × anaschata). Among the analyzed F. × ananassa cultivars, the FanAAMT gene was found in 31.6% of the tested forms, specifically in 27.3% of the 22 Russian cultivars, and 37.5% of the analyzed foreign ones. Promising sources of high methyl anthranilate content in fruits were identified: wild spp. F. moschata, F. vesca, and F. virginiana subsp. platypetala; cv. ‘Kupchikha’ (F. × anaschata); Russian garden strawberry cvs. ‘Bylinnaya’, ‘Zenit’, ‘Lastochka’, ‘Neznakomka’, ‘Feyerverk’ and ‘Tsarskoselskaya’; and foreign garden strawberry cvs. ‘Karmen’, ‘Ostara’, ‘Samson’, ‘Symphony’, ‘Troubadour’ and ‘Vima Tarda’, in which the functional allele of the FanAAMT gene was found. In the remaining studied strawberry genotypes the marker FanAAMT was not detected, which presumably indicates that the FanAAMT gene is absent. cells (R9.4.1).

аромат плодовметилантранилатмолекулярные маркерыгенотипfruit flavormethyl anthranilatemolecular markersgenotypeРабота выполнена в рамках Государственного задания согласно тематическому плану ФНЦ им. И.В. Мичурина по проекту № 0646-2019-0001 «Провести мобилизацию новых генотипов из других регионов произрастания, комплексную оценку генофонда плодовых, ягодных, нетрадиционных, цветочных культур по важнейшим селекционно значимым признакам и геномный анализ гибридных сеянцев при интрогрессивной гибридизации с целью выделения перспективных генотипов для дальнейшего селекционного использования».The research was performed within the framework of the State Task according to the theme plan of the I.V. 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The authors declare that there are no conflicts of interest present.