vir-nwТруды по прикладной ботанике, генетике и селекцииProceedings on applied botany, genetics and breeding2227-88342619-0982N.I. Vavilov All-Russian Institute of Plant Genetic Resources10.30901/2227-8834-2020-1-73-83vir-nw-550Research ArticleГЕНЕТИКА КУЛЬТУРНЫХ РАСТЕНИЙ И ИХ ДИКИХ РОДИЧЕЙGENETICS OF CULTIVATED PLANTS AND THEIR WILD RELATIVESЮжноамериканские виды Solanum alandiae Card. и S. okadae Hawkes et Hjerting как потенциальные источники генов устойчивости к фитофторозу картофеляSouth American species Solanum alandiae Card. and S. okadae Hawkes et Hjerting as potential sources of genes for potato late blight resistancehttps://orcid.org/0000-0002-6600-1693Муратова (Фадина)О. А.Muratova (Fadina)O. A.https://orcid.org/0000-0002-3104-5303БекетоваМ. П.BeketovaM. P.https://orcid.org/0000-0002-9880-5995КузнецоваМ. А.KuznetsovaM. A.

143050 Московская обл., Большие Вяземы, ул. Институт, 5

 

kuznetsova@vniif.ruhttps://orcid.org/0000-0002-2743-068XРогозинаЕ. В.RogozinaE. V.

190000 г. Санкт Петербург, ул. Б. Морская, 42, 44

 

rogozinaelena@gmail.comhttps://orcid.org/0000-0001-9177-3997ХавкинЭ. Е.KhavkinE. E.emil.khavkin@gmail.comВсероссийский научно-исследовательский институт сельскохозяйственной биотехнологии (ВНИИСБ)РоссияAll-Russian Research Institute of Agricultural BiotechnologyRussian FederationВсероссийский научно-исследовательский институт фитопатологии (ВНИИФ)РоссияAll-Russian Research Institute of PhytopathologyRussian FederationФедеральный исследовательский центр Всероссийский институт генетических ресурсов растений имени Н.И. ВавиловаРоссияN.I. Vavilov All-Russian Institute of Plant Genetic ResourcesRussian Federation20201204202018117383Copyright © Муратова (Фадина) О.А., Бекетова М.П., Кузнецова М.А., Рогозина Е.В., Хавкин Э.Е., 20202020Муратова (Фадина) О.А., Бекетова М.П., Кузнецова М.А., Рогозина Е.В., Хавкин Э.Е.Muratova (Fadina) O.A., Beketova M.P., Kuznetsova M.A., Rogozina E.V., Khavkin E.E.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://elpub.vir.nw.ru/jour/article/view/550

Дикорастущие виды Solanum L. секция Petota Dumort., сначала S. demissum Buk., а позже S. bulbocastanum Dun., S. stoloniferum Schlechtd. et Bché, S. venturii Hawkes et Hjerting (здесь и далее по системе Hawkes, 1990) и другие – служат источниками генов устойчивости к Phytophthora infestans (Mont.) de Bary (genes for resistance to P. infestans, Rpi-гены) в селекции картофеля на устойчивость к фитофторозу методами отдаленной гибридизации и транс- или цисгеноза. В отличие от Rpi-генов, детально охарактеризованных у видов Solanum из Северной и Центральной Америки, прежде всего серий Bulbocastana (Rydb.) Hawkes, Demissa Buk. и Longipedicellata Buk., Rpi-гены южноамериканских видов, включая серию Tuberosa (Rydb.) Hawkes, изучены очень слабо. К числу последних относятся S. alandiae Card. и S. okadae Hawkes et Hjerting. Четыре образца S. alandiae, образец S. okadae и 11 клонов межвидовых гибридов S. alandiae с культурным картофелем из коллекции ВИР исследовали методом ПЦР с помощью специфичных SCAR-маркеров (Sequence Characterized Amplified Region) восьми Rpi-генов. Последовательности SCAR-маркеров пяти обнаруженных Rpi-генов валидировали, сравнивая их с генами-прототипами из Генбанка NCBI. Среди структурных гомологов Rpi-генов, найденных у S. alandiae и S. okadae, особый интерес представляют гомологи Rpi-генов с широкой расовой специфичностью: R2=Rpi-blb3, R8, R9a, Rpi-vnt1 и Rpi-blb2 (соответственно 94–99, 94–99, 86–89, 92–98 и 91% сходства с генами-прототипами). Полученные данные будут способствовать лучшему пониманию дивергенции Rpi-генов в процессе эволюции клубненосных видов Solanum, особенно в серии Tuberosa.

For several decades, wild species of Solanum L. section Petota Dumort. have been involved in potato cultivar breeding for robust resistance to pests and diseases. Potato late blight (LB) is caused by oomycete Phytophthora infestans (Mont.) de Bary, and the genes for race-specific resistance to P. infestans (Rpi genes) have been introgressed into cultivated potatoes by remote crosses and trans- or cisgenesis, first from S. demissum Buk. and, more recently, from other wild species, such as S. bulbocastanum Dun., S. stoloniferum Schlechtd. et Bché, and S. venturii Hawkes et Hjerting (according to the nomenclature by Hawkes, 1990). Most wild species already involved in breeding for LB resistance came from North and Central Americas: series Bulbocastana (Rydb.) Hawkes, Demissa Buk. and Longipedicellata Buk., and some Rpi genes of these species have been already characterized in much detail. Rpi genes of South American species, including the series Tuberosa (Rydb.) Hawkes, have not been sufficiently investigated. Among the latter, this study focuses on the Rpi genes of S. alandiae Card. and S. okadae Hawkes et Hjerting. Four accessions of S. alandiae, one accession of S. okadae and 11 clones of interspecific potato hybrids comprising S. alandiae germplasm from the VIR collection were PCR-screened using specific SCAR (Sequence Characterized Amplified Region) markers for eight Rpi genes. SCAR amplicons of five Rpi genes registered in this study were validated by comparing their sequences with those of prototype genes deposited in the NCBI Genbank. Among the structural homologues of Rpi genes found in S. alandiae and S. okadae, of special interest are homologues of CC-NB-LRR resistance genes with broad specificity towards P. infestans races, in particular R2=Rpi-blb3, R8, R9a, Rpi-vnt1 and Rpi-blb2 (94–99, 94–99, 86–89, 92–98 and 91% identity with the prototype genes, respectively). Our data may help to better understand the process of Rpi gene divergence along with the evolution of tuberbearing Solanum species, particularly in the series Tuberosa.

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The authors declare that there are no conflicts of interest present.