vir-nwТруды по прикладной ботанике, генетике и селекцииProceedings on applied botany, genetics and breeding2227-88342619-0982N.I. Vavilov All-Russian Institute of Plant Genetic Resources10.30901/2227-8834-2020-1-30-47vir-nw-546Research ArticleКОЛЛЕКЦИИ МИРОВЫХ ГЕНЕТИЧЕСКИХ РЕСУРСОВ КУЛЬТУРНЫХ РАСТЕНИЙ ДЛЯ РАЗВИТИЯ ПРИОРИТЕТНЫХ НАПРАВЛЕНИЙ СЕЛЕКЦИИCOLLECTIONS OF THE WORLD’S CROP GENETIC RESOURCES FOR THE DEVELOPMENT OF PRIORITY PLANT BREEDING TRENDSРазнообразие содержания авенантрамидов у культурного и дикого овсаDiversity of avenanthramide content in wild and cultivated oatshttps://orcid.org/0000-0003-3675-6355ЛеоноваС.LeonovaS.https://orcid.org/0000-0002-5264-5594ГнутиковА.GnutikovA.

190000 г. Санкт-Петербург, ул. Б. Морская, 42, 44

 

42, 44 Bolshaya Morskaya Street, St. Petersburg 190000

https://orcid.org/0000-0002-9250-7225ЛоскутовИ.LoskutovI.

190000 г. Санкт-Петербург, ул. Б. Морская, 42, 44

 

42, 44 Bolshaya Morskaya Street, St. Petersburg 190000

i.loskutov@vir.nw.ruhttps://orcid.org/0000-0002-8898-4926БлиноваЕ.BlinovaE.

190000 г. Санкт-Петербург, ул. Б. Морская, 42, 44

 

42, 44 Bolshaya Morskaya Street, St. Petersburg 190000

ГустафссонK.-E.GustafssonK.-E.https://orcid.org/0000-0003-3962-9183ОлссонО.OlssonO.Лундский университетШвецияLund UniversitySwedenФедеральный исследовательский центр Всероссийский институт генетических ресурсов растений имени Н.И. ВавиловаРоссияN.I. Vavilov All-Russian Institute of Plant Genetic ResourcesRussian FederationШведский сельскохозяйственный университетШвецияSwedish University of Agricultural SciencesSwedenЛундский университет; CropTailor AB, Лундский университетШвецияLund University; CropTailor AB, Lund UniversitySweden20201204202018113047Copyright © Леонова С., Гнутиков А., Лоскутов И., Блинова Е., Густафссон K., Олссон О., 20202020Леонова С., Гнутиков А., Лоскутов И., Блинова Е., Густафссон K., Олссон О.Leonova S., Gnutikov A., Loskutov I., Blinova E., Gustafsson K., Olsson O.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://elpub.vir.nw.ru/jour/article/view/546Актуальность

Актуальность. Овес накапливает значительные количества различных фенольных соединений, которые обладают биологической активностью и способны значительно повышать питательную значимость продуктов из овса. Авенантрамиды (AVA) являются важной группой этих соединений благодаря их антиоксидантной, противовоспалительной и других видов активности.

Материал и методика

Материал и методика. Используя комбинированный анализ HPLC и LC-MS, впервые были получены комплексные данные по общему содержанию и составу авенантрамидов у образцов культурных и диких видов овса. Было определено  содержание  AVA  у 32 образцов  дикого и 120 образцов культурного овса, полученных из мировой коллекции Всероссийского института генетических ресурсов растений им. Н.И. Вавилова (ВИР, Санкт-Петербург, Россия).

Результаты и выводы

Результаты и выводы. Образец дикого гексаплоидного вида A. sterilis L. имел самое высокое общее содержание AVA в зерновке (1825 мг/кг), а среди образцов культурного овса самое высокое содержание AVA (586 мг/кг) имел голозерный сорт ’Numbat’ (Австралия). Данные по компонентному составу AVA показали большое разнообразие среди анализируемых образцов. Были идентифицировали образцы, у которых основные компоненты A, B и C данного вещества имели низкое содержание, а вместо них преобладали другие, менее значимые компоненты. Содержание AVA у восьми сортов овса показало значительные изменения по годам как общего содержания AVA, так и соотношения отдельных его компонентов. Используя анализ HPLC, установили и количественно определили 22 различных пика компонентов AVA в экстрактах зерновок овса. Некоторые из этих пиков, которые ранее не были документированы, предположительно представляют разные компоненты AVA. В дальнейшем необходимо детализировать полученные результаты и определить конкретные компоненты AVA в зерне овса.

Background

Background. Oat grains accumulate substantial amounts of various phenolic compounds that possess biological activity and have a potential to considerably increase health benefits of oats as a food. Avenanthramides (AVA) is an important group of these compounds due to their antioxidant, anti-itching, anti-inflammatory, antiproliferative activities.

Materials and methods

Materials and methods. Using combined HPLC and LC-MS analyses, we provide the first comprehensive review of the total avenanthramide content and composition in cultivated and wild oats. The AVA content was measured in 32 wild and 120 cultivated oat accessions obtained from the global collection of the N.I. Vavilov Institute of Plant Genetic Resources (VIR), St. Petersburg, Russia.

Results and conclusion

Results and conclusion. The wild hexaploid A. sterilis L. had the highest total AVA content, reaching 1825 mg kg–1. Among cultivated accessions, naked oat cv. ‘Numbat’ (Australia) had the highest AVA content, 586 mg kg–1. The AVA composition exhibited a wide diversity among the analyzed samples. Accessions were identified where AVAs A, B and C, which are generally considered as major AVA, had a low percentage, and instead other AVAs prevailed. The AVA content in eight oat cultivars revealed significant annual changes in both the total AVA content and the proportions of individual AVAs. Using HPLC analyses, 22 distinguishable peaks in AVA extracts of oat seeds were detected and quantified. Several of these peaks, which have not been previously documented, presumably represent different AVAs. Further analyses are needed to detail these findings and to determine the specific AVA structures in oat grains.

овескультурные и дикие виды Avenaавенантрамидыгенетические ресурсыoatcultivated and wild Avena spp.avenanthramidesgenetic resourcesThe study was supported by a STINT project entitled Improving Oat Oil Quality and Its Resistance to Pathogens (Sweden). Plant material from the VIR collection for this study was provided as part of the project No. 0662-2019-0006 (Russian Federation).ReferencesBoz H. Phenolic Amides (Avenanthramides) in Oats – A review. Czech Journal of Food Sciences. 2015;33(5):399-404. DOI: 10.17221/696/2014-CJFSBoz H. Phenolic Amides (Avenanthramides) in Oats – A review. Czech Journal of Food Sciences. 2015;33(5):399-404. DOI: 10.17221/696/2014-CJFSBratt K., Sunnerheim K., Bryngelsson S., Fagerlund A., Engman L., Andersson R.E. et al. Avenanthramides in oats (Avena sativa L.) and structure-antioxidant activity relationships. Journal of Agricultural and Food Chemistry. 2003;51(3):594-600. DOI: 10.1021/jf020544fBratt K., Sunnerheim K., Bryngelsson S., Fagerlund A., Engman L., Andersson R.E. et al. Avenanthramides in oats (Avena sativa L.) and structure-antioxidant activity relationships. Journal of Agricultural and Food Chemistry. 2003;51(3):594-600. DOI: 10.1021/jf020544fBryngelsson S., Ishihara A., Dimberg L.H. Levels of Avenanthramides and Activity of Hydroxycinnamoyl-CoA: hydroxyanthranilate N-Hydroxycinnamoyl Transferase (HHT) in Steeped or Germinated Oat Samples. Cereal Chemistry. 2003;80(3):356-360. DOI: 10.1094/CCHEM.2003.80.3.356Bryngelsson S., Ishihara A., Dimberg L.H. Levels of Avenanthramides and Activity of Hydroxycinnamoyl-CoA:hydroxyanthranilate N-Hydroxycinnamoyl Transferase (HHT) in Steeped or Germinated Oat Samples. Cereal Chemistry. 2003;80(3):356-360. DOI: 10.1094/CCHEM.2003.80.3.356Bryngelsson S., Mannerstedt-Fogelfors B., Kamal-Eldin A., Andersson R., Dimberg L.H. Lipids and antioxidants in groats and hulls of Swedish oats (Avena sativa L). Journal of the Science of Food and Agriculture. 2002;82(6):606-614. DOI: 10.1002/jsfa.1084Bryngelsson S., Mannerstedt-Fogelfors B., Kamal-Eldin A., Andersson R., Dimberg L.H. Lipids and antioxidants in groats and hulls of Swedish oats (Avena sativa L). Journal of the Science of Food and Agriculture. 2002;82(6):606-614. DOI: 10.1002/jsfa.1084Collins F.W. Oat phenolics: avenanthramides, novel substituted N-cinnamoylanthranilate alkaloids from oat groats and hulls. Journal of Agricultural and Food Chemistry. 1989;37(1):60-66. DOI: 10.1021/jf00085a015Collins F.W. Oat phenolics: avenanthramides, novel substituted N-cinnamoylanthranilate alkaloids from oat groats and hulls. Journal of Agricultural and Food Chemistry. 1989;37(1):60-66. DOI: 10.1021/jf00085a015Collins F.W. Oat Phenolics; Biochemistry and Biological Functionality. In: F.H. Webster, P.J. Woods (eds). Oats: Chemistry and Technology. 2nd ed. St. Paul, MN: AACC Inc. p.157-217.Collins F.W. Oat Phenolics; Biochemistry and Biological Functionality. In: F.H. Webster, P.J. Woods (eds). Oats: Chemistry and Technology. 2nd ed. St. Paul, MN: AACC Inc. p.157-217.Collins F.W., Burrows V.D. 2012. Method for increasing concentration of avenanthramideds in oats. USA; patent number: US2012/0082740A1; 2012.Collins F.W., Burrows V.D. 2012. Method for increasing concentration of avenanthramideds in oats. USA; patent number: US2012/0082740A1; 2012.Dimberg L.H., Gissén C., Nilsson J. Phenolic compounds in oat grains (Avena sativa L.) grown in conventional and organic systems. Ambio. 2005;34(4-5),331-337. DOI: 10.1639/0044-7447(2005)034[0331:pcioga]2.0.co;2Dimberg L.H., Gissén C., Nilsson J. Phenolic compounds in oat grains (Avena sativa L.) grown in conventional and organic systems. Ambio. 2005;34(4-5),331-337. DOI: 10.1639/0044-7447(2005)034[0331:pcioga]2.0.co;2Dimberg L.H., Molteberg E.L., Solheim R., Frølich W. Variation in Oat Groats Due to Variety, Storage and Heat Treatment. I: Phenolic Compounds. Journal of Cereal Science. 1996;24(3):263-272. DOI: 10.1006/jcrs.1996.0058Dimberg L.H., Molteberg E.L., Solheim R., Frølich W. Variation in Oat Groats Due to Variety, Storage and Heat Treatment. I: Phenolic Compounds. Journal of Cereal Science. 1996;24(3):263-272. DOI: 10.1006/jcrs.1996.0058Dimberg L.H, Sunnerheim K., Sundberg B., Walsh K. Stability of Oat Avenanthramides. Cereal Chemistry. 2001;78(3):278-281. DOI: 10.1094/CCHEM.2001.78.3.278Dimberg L.H, Sunnerheim K., Sundberg B., Walsh K. Stability of Oat Avenanthramides. Cereal Chemistry. 2001;78(3):278-281. DOI: 10.1094/CCHEM.2001.78.3.278Emmons C., Peterson D.M. Antioxidant Activity and Phenolic Content of Oat as Affected by Cultivar and Location. Crop Science. 2001;41(6):1676-1681. DOI: 10.2135/crop-sci2001.1676Emmons C., Peterson D.M. Antioxidant Activity and Phenolic Content of Oat as Affected by Cultivar and Location. Crop Science. 2001;41(6):1676-1681. DOI: 10.2135/crop-sci2001.1676Guo W., Nie L., Wu D., Wise M.L., Collins F.W., Meydani S.N., Meydani M. Avenanthramides inhibit proliferation of human colon cancer cell lines in vitro. Nutrition and Cancer. 2010;62(8):1007-1016. DOI: 10.1080/01635581.2010.492090Guo W., Nie L., Wu D., Wise M.L., Collins F.W., Meydani S.N., Meydani M. Avenanthramides inhibit proliferation of human colon cancer cell lines in vitro. Nutrition and Cancer. 2010;62(8):1007-1016. DOI: 10.1080/01635581.2010.492090Hitayezu R., Baakdah M.M., Kinnin J., Henderson K., Tsopmo A. Antioxidant activity, avenanthramide and phenolic acid contents of oat milling fractions. Journal of Cereal Science. 2015;63:35-40. DOI: 10.1016/j.jcs.2015.02.005Hitayezu R., Baakdah M.M., Kinnin J., Henderson K., Tsopmo A. Antioxidant activity, avenanthramide and phenolic acid contents of oat milling fractions. Journal of Cereal Science. 2015;63:35-40. DOI: 10.1016/j.jcs.2015.02.005Ishihara A., Miyagawa H., Kuwahara Y., Ueno T., Mayama S. Involvement of Ca 2+ ion in phytoalexin induction in oats. Plant Science. 1996;115:9-19.Ishihara A., Miyagawa H., Kuwahara Y., Ueno T., Mayama S. Involvement of Ca 2+ ion in phytoalexin induction in oats. Plant Science. 1996;115:9-19.Koenig R., Dickman J.R., Kang C., Zhang T., Chu Y.F., Ji L.L. Avenanthramide supplementation attenuates exercise-induced inflammation in postmenopausal women. Nutrition Journal. 2014;13:21. DOI: 10.1186/1475-2891-13-21Koenig R., Dickman J.R., Kang C., Zhang T., Chu Y.F., Ji L.L. Avenanthramide supplementation attenuates exercise-induced inflammation in postmenopausal women. Nutrition Journal. 2014;13:21. DOI: 10.1186/1475-2891-13-21Лоскутов И.Г. Овес (Avena L.). Распространение, систематика, эволюция и селекционная ценность. Санкт-Петербург: ВИР; 2007.Loskutov I.G. Oat (Avena L.). distribution, taxonomy, evolution and breeding value. St. Petersburg: VIR; 2007. [in Russian]Лоскутов И.Г., Ковалева О.Н., Блинова Е.В. Методические указания по изучению и сохранению мировой коллекции ячменя и овса. Санкт-Петербург: ВИР; 2012.Loskutov I.G., Kovaleva O.N., Blinova E.V. Guidelines for the study and conservation of the global collection of barley and oats (Metodicheskiye ukazaniya po izucheniyu i sokhraneniyu mirovoy kollektsii yachmenya i ovsa). St. Petersburg: VIR; 2012. [in Russian]Loskutov I.G., Rines H.W. Avena L. In: C. Kole (ed.). Wild Crop Relatives: Genomic and Breeding Resources. Heidelberg, Berlin: Springer; 2011. p.109-184. DOI: 10.1007/978-3-642-14228-4_3Loskutov I.G., Rines H.W. Avena L. In: C. Kole (ed.). Wild Crop Relatives: Genomic and Breeding Resources. Heidelberg, Berlin: Springer; 2011. p.109-184. DOI: 10.1007/978-3-642-14228-4_3Matsukawa T., Isobe T., Ishihara A., Iwamura H. Occurrence of avenanthramides and hydroxycinnamoyl-CoA:hydroxyanthranilate N-hydroxycinnamoyltransferase activity in oat seeds. Zeitschrift für Naturforschung C. A Journal of Biosciences. 2000;55(1-2):30-36. DOI: 10.1515/znc-2000-1-207Matsukawa T., Isobe T., Ishihara A., Iwamura H. Occurrence of avenanthramides and hydroxycinnamoyl-CoA:hydroxyanthranilate N-hydroxycinnamoyltransferase activity in oat seeds. Zeitschrift für Naturforschung C. A Journal of Biosciences. 2000;55(1-2):30-36. DOI: 10.1515/znc-2000-1-207Mattila P., Pihlava J.M., Hellström J. 2005. Contents of phenolic acids, alkyl- and alkenylresorcinols, and avenanthramides in commercial grain products. Journal of Agricultural and Food Chemistry. 2005;53(21): 8290–8295. DOI: 10.1021/jf051437zMattila P., Pihlava J.M., Hellström J. 2005. Contents of phenolic acids, alkyl- and alkenylresorcinols, and avenanthramides in commercial grain products. Journal of Agricultural and Food Chemistry. 2005;53(21): 8290–8295. DOI: 10.1021/jf051437zMayama S., Bordin A.P.A., Morikawa T., Tanpo H., Kato H. Association between avenalumin accumulation, infection hypha length and infection type in oat crosses segregating for resistance to Puccinia coronata f. sp. avenae race 226. Physiological and Molecular Plant Pathology. 1995a;46:255-261.Mayama S., Bordin A.P.A., Morikawa T., Tanpo H., Kato H. Association between avenalumin accumulation, infection hypha length and infection type in oat crosses segregating for resistance to Puccinia coronata f. sp. avenae race 226. Physiological and Molecular Plant Pathology. 1995a;46:255-261.Mayama S., Bordin A.P.A., Morikawa T., Tanpo H., Kato H. Association of avenalumin accumulation with co-segregation of victorin sensitivity and crown rust resistance in oat lines carrying the Pc-2 gene. Physiological and Molecular Plant Pathology. 1995b;46:263-274.Mayama S., Bordin A.P.A., Morikawa T., Tanpo H., Kato H. Association of avenalumin accumulation with co-segregation of victorin sensitivity and crown rust resistance in oat lines carrying the Pc-2 gene. Physiological and Molecular Plant Pathology. 1995b;46:263-274.Meydani M. Potential health benefits of avenanthramides of oats. Nutrition Reviews. 2009;67(12):731-735. DOI: 10.1111/j.1753-4887.2009.00256.xMeydani M. Potential health benefits of avenanthramides of oats. Nutrition Reviews. 2009;67(12):731-735. DOI: 10.1111/j.1753-4887.2009.00256.xMiyagawa H., Ishihara A., Nishimoto T., Ueno T., Mayama S. Induction of Avenanthramides in Oat Leaves Inoculated with Crown Rust Fungus, Puccinia coronata f. sp. avenae. Bioscience, Biotechnology, and Biochemistry. 1995;59(12):2305-2306. DOI: 10.1271/bbb.59.2305Miyagawa H., Ishihara A., Nishimoto T., Ueno T., Mayama S. Induction of Avenanthramides in Oat Leaves Inoculated with Crown Rust Fungus, Puccinia coronata f. sp. avenae. Bioscience, Biotechnology, and Biochemistry. 1995;59(12):2305-2306. DOI: 10.1271/bbb.59.2305Ortiz-Robledo F., Villanueva-Fierro I., Oomah B.D., Lares-Asef I., Proal-Nàjera J.B., Nàvar-Chaidez J.J. Avenanthramides and nutritional components of four Mexican oat (Avena sativa L.) varieties. Agrociencia, 2013;47(3): 225-232.Ortiz-Robledo F., Villanueva-Fierro I., Oomah B.D., Lares-Asef I., Proal-Nàjera J.B., Nàvar-Chaidez J.J. Avenanthramides and nutritional components of four Mexican oat (Avena sativa L.) varieties. Agrociencia, 2013;47(3): 225-232.Peterson D.M. Oat Antioxidants. Journal of Cereal Science. 2001;33(2):115-129. DOI: 10.1006/jcrs.2000.0349Peterson D.M. Oat Antioxidants. Journal of Cereal Science. 2001;33(2):115-129. DOI: 10.1006/jcrs.2000.0349Peterson D.M., Dimberg L.H. 2008. Avenanthramide concentrations and hydroxycinnamoyl-CoA:hydroxyanthranilate N-hydroxycinnamoyltransferase activities in developing oats. Journal of Cereal Science. 2008;47(1):101-108. DOI: 10.1016/j.jcs.2007.02.007Peterson D.M., Dimberg L.H. 2008. Avenanthramide concentrations and hydroxycinnamoyl-CoA:hydroxyanthranilate N-hydroxycinnamoyltransferase activities in developing oats. Journal of Cereal Science. 2008;47(1):101-108. DOI: 10.1016/j.jcs.2007.02.007Peterson D.M., Wesenberg D.M., Burrup D.E., Erikson C.A. Relationships among Agronomic Traits and Grain Composition in Oat Genotypes Grown in Different Environments. Crop Science. 2005;45:1249-1255. DOI: 10.2135/cropsci2004.0063Peterson D.M., Wesenberg D.M., Burrup D.E., Erikson C.A. Relationships among Agronomic Traits and Grain Composition in Oat Genotypes Grown in Different Environments. Crop Science. 2005;45:1249-1255. DOI: 10.2135/cropsci2004.0063Redaelli R., Dimberg L., Germeier C.U., Berardo N., Locatelli S., Guerrini L. Variability of tocopherols, tocotrienols and avenanthramides contents in European oat germplasm. Euphytica. 2016;207:273-292. DOI: 10.1007/s10681-015-1535-8Redaelli R., Dimberg L., Germeier C.U., Berardo N., Locatelli S., Guerrini L. Variability of tocopherols, tocotrienols and avenanthramides contents in European oat germplasm. Euphytica. 2016;207:273-292. DOI: 10.1007/s10681-015-1535-8Ren Y., Wise M. Avenanthramide Biosynthesis in Oat Cultivars Treated with Systemic Acquired Resistance Elicitors. Cereal Research Communications. 2012;41(2):255-265. DOI: 10.1556/CRC.2012.0035Ren Y., Wise M. Avenanthramide Biosynthesis in Oat Cultivars Treated with Systemic Acquired Resistance Elicitors. Cereal Research Communications. 2012;41(2):255-265. DOI: 10.1556/CRC.2012.0035Ren Y., Yang X., Niu X., Liu S., Ren G. Chemical characterization of the avenanthramide-rich extract from oat and its effect on D-galactose-induced oxidative stress in mice. Journal of Agricultural and Food Chemistry. 2011;59(1):206-211. DOI: 10.1021/jf103938eRen Y., Yang X., Niu X., Liu S., Ren G. Chemical characterization of the avenanthramide-rich extract from oat and its effect on D-galactose-induced oxidative stress in mice. Journal of Agricultural and Food Chemistry. 2011;59(1):206-211. DOI: 10.1021/jf103938eРодионова Н.А., Солдатов В.Н., Мережко В.Е., Ярош Н.П., Кобылянский В.Д. Культурная флора. Т. 2, ч. 3. Овес. Москва: Колос; 1994.Rodionova N.A., Soldatov V.N., Merezhko V.E., Yarosh N.P., Kobylyansky V.D. Flora of cultivated plants. Vol. 2 (Pt 3). Oat (Kulturnaya flora. T. 2, ch. 3. Oves). Moscow: Kolos; 1994. [in Russian]Wang P., Chen H., Zhu Y., McBride J., Fu J., Sang S. Oat Avenanthramide-C (2c) Is Biotransformed by Mice and the Human Microbiota into Bioactive Metabolites. The Journal of Nutrition. 2014;145(2):239-245. DOI: 10.3945/jn.114.206508Wang P., Chen H., Zhu Y., McBride J., Fu J., Sang S. Oat Avenanthramide-C (2c) Is Biotransformed by Mice and the Human Microbiota into Bioactive Metabolites. The Journal of Nutrition. 2014;145(2):239-245. DOI: 10.3945/jn.114.206508Wise M.L. Avenanthramides: Chemistry and Biosynthesis. In: Y. Chu (ed.). Oats Nutrition and Technology. Chichester, UK: John Wiley & Sons Ltd.; 2014. p.195-226. DOI: 10.1002/9781118354100.ch8Wise M.L. Avenanthramides: Chemistry and Biosynthesis. In: Y. Chu (ed.). Oats Nutrition and Technology. Chichester, UK: John Wiley & Sons Ltd.; 2014. p.195-226. DOI: 10.1002/9781118354100.ch8Wise M.L. Effect of chemical systemic acquired resistance elicitors on avenanthramide biosynthesis in oat (Avena sativa). Journal of Agricultural and Food Chemistry. 2011;59(13):7028-7038. DOI: 10.1021/jf2008869Wise M.L. Effect of chemical systemic acquired resistance elicitors on avenanthramide biosynthesis in oat (Avena sativa). Journal of Agricultural and Food Chemistry. 2011;59(13):7028-7038. DOI: 10.1021/jf2008869Wise M.L., Doehlert D.C., McMullen M.S. Association of Avenanthramide Concentration in Oat (Avena sativa L.) Grain with Crown Rust Incidence and Genetic Resistance. Cereal Chemistry. 2008;85(5):639-641. DOI: 10.1094/CCHEM-85-5-0639Wise M.L., Doehlert D.C., McMullen M.S. Association of Avenanthramide Concentration in Oat (Avena sativa L.) Grain with Crown Rust Incidence and Genetic Resistance. Cereal Chemistry. 2008;85(5):639-641. DOI: 10.1094/CCHEM-85-5-0639Yang J., Ou B., Wise M.L., Chu Y. In vitro total antioxidant capacity and anti-inflammatory activity of three common oat-derived avenanthramides. Food Chemistry. 2014;160(1):338–345. DOI: 10.1016/j.foodchem.2014.03.059Yang J., Ou B., Wise M.L., Chu Y. In vitro total antioxidant capacity and anti-inflammatory activity of three common oat-derived avenanthramides. Food Chemistry. 2014;160(1):338–345. DOI: 10.1016/j.foodchem.2014.03.059

The authors declare that there are no conflicts of interest present.