vir-nwТруды по прикладной ботанике, генетике и селекцииProceedings on applied botany, genetics and breeding2227-88342619-0982N.I. Vavilov All-Russian Institute of Plant Genetic Resources10.30901/2227-8834-2015-2-225-236vir-nw-34Research ArticleИДЕНТИФИКАЦИЯ ГЕНЕТИЧЕСКОГО РАЗНООБРАЗИЯ КУЛЬТУРНЫХ РАСТЕНИЙ И ИХ ДИКИХ РОДИЧЕЙ ДЛЯ РЕШЕНИЯ ФУНДАМЕНТАЛЬНЫХ И ПРИКЛАДНЫХ ПРОБЛЕМIDENTIFICATION OF THE DIVERSITY OF CULTIVATED PLANTS AND THEIR WILD RELATIVES FOR SOLVING FUNDAMENTAL AND APPLIED PROBLEMSПОЛИМОРФИЗМ ГЕНА, КОДИРУЮЩЕГО АНИОННУЮ ПЕРОКСИДАЗУ ПШЕНИЦЫANIONIC PEROXIDASE GENE POLYMORPHISM IN WHEATМаксимовИ. В.MaksimovI. V.maksimov@ufaras.ruБурхановаГ. Ф.BurkhanovaG. F.noemail@neicon.ruКузьминаО. И.KuzminaO. I.noemail@neicon.ruВахитовВ. А.VakhitovV. A.noemail@neicon.ruИнститут биохимии и генетики Уфимского научного центра РАНРоссияInstitute of Biochemistry and Genetics of Ufa Science Centre of Russian Academy of SciencesRussian Federation2015170620181762225236Copyright © Максимов И.В., Бурханова Г.Ф., Кузьмина О.И., Вахитов В.А., 20182018Максимов И.В., Бурханова Г.Ф., Кузьмина О.И., Вахитов В.А.Maksimov I.V., Burkhanova G.F., Kuzmina O.I., Vakhitov V.A.This work is licensed under a Creative Commons Attribution 4.0 License.https://elpub.vir.nw.ru/jour/article/view/34

В результате секвенирования и анализа структуры фрагмента гена AK333699 у разных видов трибы Triticeae Trin. ex Griseb. показано, что ген анионной пероксидазы характеризуется сходной организацией внутри рода пшениц. При этом происходит разделение видов пшениц на два отдельных кластера, соответствующих их геномному распределению на два подрода - Boeoticum Migusch. et Dorof. и Triticum. Таким образом, секвенированные фрагменты, гомологичные гену TC151917 (код доступа AK333699.1) анионной пероксидазы Triticum aestivum L., и подобные мотивы пероксидаз у родственных видов растений можно выделить в отдельный кластер полисахарид-специфичных изопероксидаз, которые, вероятно, могут взаимодействовать с клеточными стенками патогенных грибов и запускать защитные механизмы растения в ответ на инфицирование.

The results postulated a close genetic proximity of the peroxidase gene AK333699 of Triticum aestivum L. among Triticum L. species. At the same time - T. compactum Host, T. durum Desf., T. macha Dekapr. & Menabde, T. spelta L., T. turgidum L., T. urartu Thum. ex Gandiljan and T. aestivum (media genomes Au) clustered to the subgenus Triticum. Species T. compactum, T. durum, T. macha, T. spelta, T. turgidum, T. urartu and T. aestivum (media genomes Ab) clustered together to the subgenus Boeoticum Migusch. et Dorof. Thus, the sequenced fragments homologous to the peroxidase gene TC151917 of T. aestivum and the similar peroxidase gene in another species can be identified as a separate cluster of polysaccharide-specific isoperoxidases which can bind on pathogens cell wall structures and initiate plant defense mechanisms.

виды пшениц и эгилопсген пероксидазыполиморфизмwheatTriticum and Aegilops speciesgene of peroxidaseReferencesAlmagro L., Gomez Ros L. V., Belchi-Navarro S. et all. Class III peroxidases in plant defence reactions // J. Exp. Botany. 2009. Vol. 60. P. 377-390Almagro L., Gomez Ros L. V., Belchi-Navarro S. et all. Class III peroxidases in plant defence reactions // J. Exp. Botany. 2009. Vol. 60. P. 377-390Bacalovic N., Passardi F., Ioannidis V. et all. PeroxiBase: A class III plant peroxidase database // Phytochemistry. 2006. Vol. 67. P. 534-539Bacalovic N., Passardi F., Ioannidis V. et all. PeroxiBase: A class III plant peroxidase database // Phytochemistry. 2006. Vol. 67. P. 534-539Blee K. A., Choi J. W., O’Connell A. P. et all. A lignin-specifc peroxidase in tobacco whose antisense suppression leads to vascular tissue modification // Phytochemistry, 2003. Vol. 64. P. 163-176Blee K. A., Choi J. W., O’Connell A. P. et all. A lignin-specifc peroxidase in tobacco whose antisense suppression leads to vascular tissue modification // Phytochemistry, 2003. Vol. 64. P. 163-176Carpin S., Crevecoeur M., Meyer M. et all. Identification of a Ca2-pectate binding site on an apoplastic peroxidase // Plant Cell. 2001. Vol. 13. P. 511-520Carpin S., Crevecoeur M., Meyer M. et all. Identification of a Ca2-pectate binding site on an apoplastic peroxidase // Plant Cell. 2001. Vol. 13. P. 511-520Cosio C., Vuillemin L., Meyer M. et all. An anionic class III peroxidase from zucchini may regulate hypocotyl elongation through its auxin oxidase activity // Planta, 2009. Vol. 229. P. 823-836Cosio C., Vuillemin L., Meyer M. et all. An anionic class III peroxidase from zucchini may regulate hypocotyl elongation through its auxin oxidase activity // Planta, 2009. Vol. 229. P. 823-836Dorofeev V. F., Udachin R. A. et all. Wheats of the World. Leningrad: Kolos, 1987. 560 p. (in Russian)Dorofeev V. F., Udachin R. A. et all. Wheats of the World. Leningrad: Kolos, 1987. 560 p. (in Russian)Dunand C, Tognolli M., Meyer M. et all. Identification and Characterization of Ca - Pectate Binding Peroxidase in Arabidopsis thailiana // Journal of Plant Physiology. 2002. Vol. 159. P. 1165-1171Dunand C, Tognolli M., Meyer M. et all. Identification and Characterization of Ca - Pectate Binding Peroxidase in Arabidopsis thailiana // Journal of Plant Physiology. 2002. Vol. 159. P. 1165-1171Goncharov N., Kondratenko E. Origin, domestication and evolution of wheat // The Vavilov Journal of Genetics and Breading, 2008. Vol. 12. N 1, 2. P. 159-179. (in Russian)Goncharov N., Kondratenko E. Origin, domestication and evolution of wheat // The Vavilov Journal of Genetics and Breading, 2008. Vol. 12. N 1, 2. P. 159-179. (in Russian)Hiraga S., Sasaki K., Ito H. et all. A large family of class III plant peroxidases // Plant Cell Physiol., 2001. Vol 42. P. 462-468Hiraga S., Sasaki K., Ito H. et all. A large family of class III plant peroxidases // Plant Cell Physiol., 2001. Vol 42. P. 462-468Kawano T. Roles of the reactive oxygen species-generating peroxidase reactions in plant defense and growth induction // Plant Cell Rep. 2003. Vol. 21. P. 829837Kawano T. Roles of the reactive oxygen species-generating peroxidase reactions in plant defense and growth induction // Plant Cell Rep. 2003. Vol. 21. P. 829837Liu G., Sheng X., Greenshields D., Ogieglo A. Profiling of wheat class III peroxidase genes derived from mildew-attacked epidermis reveals distinct sequence-associated expression patterns // Molecular Plant-Microbe Interaction. 2005. Vol. 18. P. 730-741Liu G., Sheng X., Greenshields D., Ogieglo A. Profiling of wheat class III peroxidase genes derived from mildew-attacked epidermis reveals distinct sequence-associated expression patterns // Molecular Plant-Microbe Interaction. 2005. Vol. 18. P. 730-741Maksimov . V., Cherepanova E. A., Yarullina L. G., Axmetova I. E. Isolation of chitin-specific wheat oxidoreductases // Applied Biochemistry and Microbiology. 2005. Vol. 41, N 6. P. 616-620. (in Russian)Maksimov . V., Cherepanova E. A., Yarullina L. G., Axmetova I. E. Isolation of chitin-specific wheat oxidoreductases // Applied Biochemistry and Microbiology. 2005. Vol. 41, N 6. P. 616-620. (in Russian)Passardi F., Penel C., Dunand C. Performing the paradoxical: how plant peroxidases modify the cell wall // Plant Science. 2004. Vol. 9. P. 534-540Passardi F., Penel C., Dunand C. Performing the paradoxical: how plant peroxidases modify the cell wall // Plant Science. 2004. Vol. 9. P. 534-540Passardi F., Cosio C., Penel C., Dunand C. Peroxidases have more functions than a Swiss army knife // Plant Cell Rep. 2005. Vol. 24. P. 255-265Passardi F., Cosio C., Penel C., Dunand C. Peroxidases have more functions than a Swiss army knife // Plant Cell Rep. 2005. Vol. 24. P. 255-265Passardi F., Theiler G., Zamocky M. et all. PeroxiBase: the peroxidase database // Phytochemistry. 2007. Vol. 68. P. 1605-1611Passardi F., Theiler G., Zamocky M. et all. PeroxiBase: the peroxidase database // Phytochemistry. 2007. Vol. 68. P. 1605-1611Penel C., Van Cutsem P., Greppin H. Interactions of a plant peroxidase with oligogalacturonides in the presence of calcium ions // Phytochemistry. 1999. Vol. 51. P. 193-198Penel C., Van Cutsem P., Greppin H. Interactions of a plant peroxidase with oligogalacturonides in the presence of calcium ions // Phytochemistry. 1999. Vol. 51. P. 193-198Penel C., Dunand C. Signaling via Plant Peroxidases // Baluska F, Vivanco J, Eds. Signaling in Plants. Springer Berlin Heidelberg, 2009Penel C., Dunand C. Signaling via Plant Peroxidases // Baluska F, Vivanco J, Eds. Signaling in Plants. Springer Berlin Heidelberg, 2009Pshenichnov E., Khashimova N., Akhunov A. et all. Participation of Chitin-Binding Peroxidase Isoforms in the Wilt Pathogenesis of Cotton // American Journal of Plant Sciences. 2011. Vol. 2. P. 43-49Pshenichnov E., Khashimova N., Akhunov A. et all. Participation of Chitin-Binding Peroxidase Isoforms in the Wilt Pathogenesis of Cotton // American Journal of Plant Sciences. 2011. Vol. 2. P. 43-49Schweikert C., Liszkay A., Schopher P. Polysaccharide Degradation by Fenton Reaction- or Peroxidase- Gene-Rated Hydroxyl Radicals in Isolated Plant Cell Walls // Phytochemistry. 2002. Vol. 61. P. 31-35Schweikert C., Liszkay A., Schopher P. Polysaccharide Degradation by Fenton Reaction- or Peroxidase- Gene-Rated Hydroxyl Radicals in Isolated Plant Cell Walls // Phytochemistry. 2002. Vol. 61. P. 31-35Shah K., Penel C., Gagnon J., Dunand C. Purification and identification of a Ca-pectate binding peroxidase from Arabidopsis leaves // Phytochemistry. 2004. Vol. 65. P. 307-312Shah K., Penel C., Gagnon J., Dunand C. Purification and identification of a Ca-pectate binding peroxidase from Arabidopsis leaves // Phytochemistry. 2004. Vol. 65. P. 307-312

The authors declare that there are no conflicts of interest present.