vir-nwТруды по прикладной ботанике, генетике и селекцииProceedings on applied botany, genetics and breeding2227-88342619-0982N.I. Vavilov All-Russian Institute of Plant Genetic Resources10.30901/2227-8834-2026-1-o12vir-nw-2429Research ArticleОБЗОРЫSURVEYSАллельное разнообразие генов короткостебельности ячменя HvGA20ox2, Sln1, HvBRI1 и HvDep1 – интеграция литературных данных и результатов анализа пангенома in silicoAllelic diversity of the barley semidwarfing genes HvGA20ox2, Sln1, HvBRI1, and HvDep1: integrating published data and results of the in silico pangenome analysishttps://orcid.org/0000-0001-5841-8803ПоротниковИ. В.PorotnikovI. V.

Игорь Вадимович Поротников, научный сотрудник,  ВИР

190000 Россия, Санкт-Петербург, ул. Б. Морская, 42, 44

Igor V. Porotnikov, Researcher, VIR

42, 44 Bolshaya Morskaya Street, St. Petersburg 190000, Russia

i.v.porotnikov@gmail.comhttps://orcid.org/0000-0001-5477-8684ЛукинаК. А.LukinaK. A.

Ксения Андреевна Лукина, научный сотрудник, ВИР

190000 Россия, Санкт-Петербург, ул. Б. Морская, 42, 44

Kseniia A. Lukina, Researcher, VIR

42, 44 Bolshaya Morskaya Street, St. Petersburg 190000, Russia

 

k.lukina@vir.nw.ruhttps://orcid.org/0000-0001-8334-8069АнтоноваО. Ю.AntonovaO. Yu.

Ольга Юрьевна Антонова, кандидат биологических наук, ведущий научный сотрудник, ВИР

190000 Россия, Санкт-Петербург, ул. Б. Морская, 42, 44

Olga Yu. Antonova, Cand. Sci. (Biology), Leading Researcher,  VIR

42, 44 Bolshaya Morskaya Street, St. Petersburg 190000, Russia

olgaant326@mail.ruhttps://orcid.org/0000-0002-3990-6526КовалеваО. Н.KovalevaO. N.

Ольга Николаевна Ковалева, кандидат биологических наук, ведущий научный сотрудник, ВИР

190000 Россия, Санкт-Петербург, ул. Б. Морская, 42, 44

Olga N. Kovaleva, Cand. Sci. (Biology), Leading Researcher, VIR

42, 44 Bolshaya Morskaya Street, St. Petersburg 190000, Russia

o.kovaleva@vir.nw.ruФедеральный исследовательский центр Всероссийский институт генетических ресурсов растений имени Н.И. ВавиловаРоссияN.I. Vavilov All-Russian Institute of Plant Genetic ResourcesRussian Federation2026300320261871262276Copyright © Поротников И.В., Лукина К.А., Антонова О.Ю., Ковалева О.Н., 20262026Поротников И.В., Лукина К.А., Антонова О.Ю., Ковалева О.Н.Porotnikov I.V., Lukina K.A., Antonova O.Y., Kovaleva O.N.This work is licensed under a Creative Commons Attribution 4.0 License.https://elpub.vir.nw.ru/jour/article/view/2429

Настоящий обзор посвящен анализу полиморфизма генов короткостебельности HvGA20ox2, HvBRI1 и HvDep1 культурного ячменя, а также перспективного в этом плане гена Sln1. Обобщены данные о механизме действия и роли в селекции их аллелей, как природных, так и индуцированных. С целью расширения сведений о полиморфизме генов HvGA20ox2, Sln1, HvBRI1 и HvDep1 в обзор интегрированы результаты анализа in silico их последовательностей в пангеноме культурного ячменя, включающего 53 генотипа. В результате выявлен спектр ранее неизвестных гаплотипов и мутаций, потенциально ассоциированных с признаком высоты растений. Для гена HvGA20ox2 идентифицировано 10 гаплотипов промоторной области и 6 типов белка, включая известные аллели sdw1.c, sdw1.d, sdw1.ins и sdw1.ZU9, причем последние два дифференцированы на подтипы. Ген Sln1 характеризовался высокой консервативностью кодирующей области (изменения обнаружены лишь у 3 генотипов) при значительном разнообразии промотора (13 гаплотипов). Для гена HvBRI1 выявлен аллель короткостебельности uzu1.a, однако в целом для него характерен наименьший полиморфизм как промоторной, так и кодирующей области. Для гена HvDep1 показана максимальная вариабельность промотора (81% образцов отличались от дикого типа) при высокой консервативности белка. Таким образом, полученные сведения расширяют возможности направленного использования выявленного генетического разнообразия для дальнейших фундаментальных и прикладных исследований в селекции ячменя.

This review is focused on analyzing the polymorphism of the dwarfing genes HvGA20ox2, HvBRI1, and HvDep1 in cultivated barley, as well as the Sln1 gene, which is promising in this regard. Data on the mechanism of action and the role of their natural and induced alleles in barley breeding are summarized. To expand our understanding of the polymorphism of HvGA20ox2, Sln1, HvBRI1, and HvDep1, this review integrates the results of the in silico analysis of their sequences in the cultivated barley pangenome, which includes 53 genotypes. This approach revealed a spectrum of previously uncharacterized haplotypes and mutations potentially associated with plant height. For HvGA20ox2, 10 promoter haplotypes and 6 protein types were identified, including the known alleles sdw1.c, sdw1.d, sdw1.ins, and sdw1.ZU9, with the latter two differentiated into subtypes. The Sln1 gene was characterized by a highly conserved coding region (changes were detected in only three genotypes), whereas the promoter region exhibited considerable variability, with 13 haplotypes. For HvBRI1, the uzu1.a dwarfism allele was identified; however, this gene is characterized by the lowest polymorphism of both the promoter and coding regions. The highest promoter variability (81% of the accessions differed from the wild type) with highly conserved protein was demonstrated for HvDep1. Thus, these data expand opportunities for the targeted application of the identified genetic diversity in further fundamental and applied research in barley breeding.

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The authors declare that there are no conflicts of interest present.