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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">vir-nw</journal-id><journal-title-group><journal-title xml:lang="ru">Труды по прикладной ботанике, генетике и селекции</journal-title><trans-title-group xml:lang="en"><trans-title>Proceedings on applied botany, genetics and breeding</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2227-8834</issn><issn pub-type="epub">2619-0982</issn><publisher><publisher-name>N.I. Vavilov All-Russian Institute of Plant Genetic Resources</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.30901/2227-8834-2025-3-37-56</article-id><article-id custom-type="elpub" pub-id-type="custom">vir-nw-2412</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>МОБИЛИЗАЦИЯ И СОХРАНЕНИЕ ГЕНЕТИЧЕСКОГО РАЗНООБРАЗИЯ КУЛЬТУРНЫХ РАСТЕНИЙ И ИХ ДИКИХ РОДИЧЕЙ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>MOBILIZATION AND CONSERVATION OF THE GENETIC DIVERSITY OF CULTIVATED PLANTS AND THEIR WILD RELATIVES</subject></subj-group></article-categories><title-group><article-title>Изучение и создание коллекций in vitro редких исчезающих растений семейства Rosaceae</article-title><trans-title-group xml:lang="en"><trans-title>Studying and setting up in vitro collections of rare endangered plants of the Rosaceae family</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-0258-9448</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Земцова</surname><given-names>А. С.</given-names></name><name name-style="western" xml:lang="en"><surname>Zemtsova</surname><given-names>A. S.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Алина Сергеевна Земцова, младший научный сотрудник</p><p>050040, Алматы, ул. Тимирязева, 45</p></bio><bio xml:lang="en"><p>Alina S. Zemtsova, Associate Researcher</p><p>45 Timiryazev St., Almaty 050040</p></bio><email xlink:type="simple">zemtsovaaalina@gmail.com</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-5039-8999</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Алтаева</surname><given-names>Н. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Altayeva</surname><given-names>N. N.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Назира Алтайқызы Алтаева, старший научный сотрудник</p><p>050040, Алматы, ул. Тимирязева, 45</p></bio><bio xml:lang="en"><p>Nazira A. Altayeva, Senior Researcher</p><p>45 Timiryazev St., Almaty 050040</p></bio><email xlink:type="simple">altnazira@gmail.com</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-2322-8054</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Аралбаева</surname><given-names>М. М.</given-names></name><name name-style="western" xml:lang="en"><surname>Aralbaeva</surname><given-names>M. M.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Молдир Маликовна Аралбаева, научный сотрудник</p><p>050040, Алматы, ул. Тимирязева, 45</p></bio><bio xml:lang="en"><p>Moldir M. Aralbaeva, Researcher</p><p>45 Timiryazev St., Almaty 050040</p></bio><email xlink:type="simple">berim.moldir@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0009-0004-5354-8069</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Артимович</surname><given-names>Н. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Artimovich</surname><given-names>N. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Наталья Анатольевна Артимович, лаборант</p><p>050040, Алматы, ул. Тимирязева, 45</p></bio><bio xml:lang="en"><p>Natalya A. Artimovich, Laboratory Assistant</p><p>45 Timiryazev St., Almaty 050040</p></bio><email xlink:type="simple">artimovich_nata@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-3747-2330</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Толеген</surname><given-names>А. Б.</given-names></name><name name-style="western" xml:lang="en"><surname>Tolegen</surname><given-names>A. B.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Арман Болатханулы Толеген, старший научный сотрудник, Институт биологии и биотехнологии растений; докторант, Казахский национальный университет имени аль-Фараби</p><p>050040, Алматы, ул. Тимирязева, 45</p><p>050040, Алматы, пр. Аль-Фараби, 71</p></bio><bio xml:lang="en"><p>Arman B. Tolegen, Senior Researcher, Institute of Plant Biology and Biotechnology; PhD Student, al-Farabi Kazakh National University</p><p>45 Timiryazev St., Almaty 05004</p><p>71 Al-Farabi Ave., Almaty 050040</p></bio><email xlink:type="simple">tolegenarman7@gmail.com</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-1926-0091</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Кушнаренко</surname><given-names>С. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Kushnarenko</surname><given-names>S. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Светлана Вениаминовна Кушнаренко, кандидат биологических наук, профессор, заведующая лабораторией</p><p>050040, Алматы, ул. Тимирязева, 45</p></bio><bio xml:lang="en"><p>Svetlana V. Kushnarenko, Cand. Sci. (Biology), Professor, Head of a Laboratory</p><p>45 Timiryazev St., Almaty 050040</p></bio><email xlink:type="simple">svetlana_bio@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-6625-0043</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Сатеков</surname><given-names>Е. Я.</given-names></name><name name-style="western" xml:lang="en"><surname>Satekov</surname><given-names>Ye. Ya.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Ескендир Яковлевич Сатеков, кандидат биологических наук, научный сотрудник</p><p>050040, Алматы, ул. Тимирязева, 45</p></bio><bio xml:lang="en"><p>Yeskendir Ya. Satekov, Cand. Sci. (Biology), Researcher</p><p>45 Timiryazev St., Almaty 050040</p></bio><email xlink:type="simple">irokezz@inbox.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-0792-1086</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Премина</surname><given-names>Н. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Premina</surname><given-names>N. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Наталья Викторовна Премина, старший научный сотрудник</p><p>070000, Риддер, Семипалатинская ул., 9</p></bio><bio xml:lang="en"><p>Natalya V. Premina, Senior Researcher</p><p>9 Semipalatinskaya St., Ridder 070000</p></bio><email xlink:type="simple">Preminanv@mail.ru</email><xref ref-type="aff" rid="aff-3"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-1052-2753</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Ромаданова</surname><given-names>Н. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Romadanova</surname><given-names>N. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Наталья Владимировна Ромаданова, кандидат биологических наук, ассоциированный профессор, ведущий научный сотрудник</p><p>050040, Алматы, ул. Тимирязева, 45</p></bio><bio xml:lang="en"><p>Natalya V. Romadanova, Cand. Sci. (Biology), Associate Professor, Leading Researcher</p><p>45 Timiryazev St., Almaty 050040</p></bio><email xlink:type="simple">nata_romadanova@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru">Институт биологии и биотехнологии растений<country>Казахстан</country></aff><aff xml:lang="en">Institute of Plant Biology and Biotechnology<country>Kazakhstan</country></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru">Институт биологии и биотехнологии растений; Казахский национальный университет имени аль-Фараби<country>Казахстан</country></aff><aff xml:lang="en">Institute of Plant Biology and Biotechnology; Al-Farabi Kazakh National University<country>Kazakhstan</country></aff></aff-alternatives><aff-alternatives id="aff-3"><aff xml:lang="ru">Западно-Алтайский государственный природный заповедник<country>Казахстан</country></aff><aff xml:lang="en">West Altai State Nature Reserve<country>Kazakhstan</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2025</year></pub-date><pub-date pub-type="epub"><day>30</day><month>09</month><year>2025</year></pub-date><volume>186</volume><issue>3</issue><fpage>37</fpage><lpage>56</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Земцова А.С., Алтаева Н.А., Аралбаева М.М., Артимович Н.А., Толеген А.Б., Кушнаренко С.В., Сатеков Е.Я., Премина Н.В., Ромаданова Н.В., 2025</copyright-statement><copyright-year>2025</copyright-year><copyright-holder xml:lang="ru">Земцова А.С., Алтаева Н.А., Аралбаева М.М., Артимович Н.А., Толеген А.Б., Кушнаренко С.В., Сатеков Е.Я., Премина Н.В., Ромаданова Н.В.</copyright-holder><copyright-holder xml:lang="en">Zemtsova A.S., Altayeva N.N., Aralbaeva M.M., Artimovich N.A., Tolegen A.B., Kushnarenko S.V., Satekov Y.Y., Premina N.V., Romadanova N.V.</copyright-holder><license license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://elpub.vir.nw.ru/jour/article/view/2412">https://elpub.vir.nw.ru/jour/article/view/2412</self-uri><abstract><sec><title>Актуальность</title><p>Актуальность. Актуальность обусловлена необходимостью сохранения генетического разнообразия редких и исчезающих растений семейства Rosaceae, которые играют важную роль в экосистемах и имеют ценность для сельского хозяйства, медицины и декоративного садоводства.</p></sec><sec><title>Материалы и методы</title><p>Материалы и методы. Материалом для исследования послужили 9 видов растений семейства Rosaceae, занесенных в Красную книгу Казахстана.</p></sec><sec><title>Результаты</title><p>Результаты. В результате экспедиций собрано девять видов растений семейства Rosaceae. Для шести видов оптимизирована методология введения в культуру in vitro и микроклонального размножения на основе использования питательной среды Мурасиге и Скуга с добавлением регуляторов роста.</p></sec><sec><title>Заключение</title><p>Заключение. Созданная коллекция in vitro редких и исчезающих растений семейства Rosaceae является эффективным инструментом для их сохранения и воспроизводства. Эти методы обеспечивают долгосрочное сохранение биоразнообразия и создают возможности для дальнейшего использования генетического материала в научных и прикладных целях.</p></sec></abstract><trans-abstract xml:lang="en"><sec><title>Background</title><p>Background. The relevance is based on the need to preserve the genetic diversity of rare and endangered Rosaceae plants that play an important role in ecosystems and are of value for agriculture, medicine, and ornamental gardening.</p></sec><sec><title>Materials and methods</title><p>Materials and methods. Nine plant species from the Rosaceae family listed in the Red Data Book of Kazakhstan served as the material for this study.</p></sec><sec><title>Results</title><p>Results. Accessions of 9 Rosaceae species were collected by plant collecting missions. The in vitro initiation and micropropagation techniques employing the Murashige and Skoog medium with added growth regulators was optimized for 6 species.</p></sec><sec><title>Conclusion</title><p>Conclusion. The established in vitro collection of rare and endangered plants from the Rosaceae family is an effective tool for their conservation and reproduction. The applied techniques ensure long-term conservation of biodiversity and create opportunities for further use of genetic material for scientific and practical purposes.</p></sec></trans-abstract><kwd-group xml:lang="ru"><kwd>Rosaceae</kwd><kwd>Красная книга Казахстана</kwd><kwd>геоботаническое изучение популяций</kwd><kwd>дескрипторы</kwd><kwd>коллекция in vitro</kwd></kwd-group><kwd-group xml:lang="en"><kwd>Rosaceae</kwd><kwd>Red Data Book</kwd><kwd>geobotanical population study</kwd><kwd>descriptors</kwd><kwd>in vitro collection</kwd></kwd-group><funding-group xml:lang="ru"><funding-statement>Работа выполнена при поддержке грантового финансирования проекта АР19676010 Министерства науки и высшего образования Республики Казахстан. Авторы благодарят рецензентов за их вклад в экспертную оценку этой работы.</funding-statement></funding-group><funding-group xml:lang="en"><funding-statement>The study was supported from the grant-funded project AP19676010 of the Ministry of Science and Higher Education of the Republic of Kazakhstan.  The authors thank the reviewers for their contribution to the peer review of this work.</funding-statement></funding-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Afifi L., Lapin K., Tremetsberger K., Konrad H. A systematic review of threats, conservation, and management measures for tree species of the family Rosaceae in Europe. Flora. 2023;301:152244. DOI: 10.1016/j.flora.2023.152244</mixed-citation><mixed-citation xml:lang="en">Afifi L., Lapin K., Tremetsberger K., Konrad H. A systematic review of threats, conservation, and management measures for tree species of the family Rosaceae in Europe. Flora. 2023;301:152244. DOI: 10.1016/j.flora.2023.152244</mixed-citation></citation-alternatives></ref><ref id="cit2"><label>2</label><citation-alternatives><mixed-citation xml:lang="ru">Bonnart R.M., Chen K.Y., Volk G.M. Plant tissue culture media preparation. In: G.M. Volk, K. Chen (eds). Training in Plant Genetic Resources: Cryopreservation of Clonal Propagules. Fort Collins, CO: Colorado State University: 2022. Available from: https://colostate.pressbooks.pub/ clonalcryopreservation/chapter/media [accessed Jan. 21, 2025].</mixed-citation><mixed-citation xml:lang="en">Bonnart R.M., Chen K.Y., Volk G.M. Plant tissue culture media preparation. In: G.M. Volk, K. Chen (eds). Training in Plant Genetic Resources: Cryopreservation of Clonal Propagules. Fort Collins, CO: Colorado State University: 2022. Available from: https://colostate.pressbooks.pub/ clonalcryopreservation/chapter/media [accessed Jan. 21, 2025].</mixed-citation></citation-alternatives></ref><ref id="cit3"><label>3</label><citation-alternatives><mixed-citation xml:lang="ru">Coelho N., Gonçalves S., Romano A. Endemic plant species conservation: Biotechnological approaches. Plants. 2020;9(3):345. DOI: 10.3390/plants9030345</mixed-citation><mixed-citation xml:lang="en">Coelho N., Gonçalves S., Romano A. Endemic plant species conservation: Biotechnological approaches. Plants. 2020;9(3):345. DOI: 10.3390/plants9030345</mixed-citation></citation-alternatives></ref><ref id="cit4"><label>4</label><citation-alternatives><mixed-citation xml:lang="ru">Country report on the state of plant genetic resources for food and agriculture in the Kazakhstan Republic. Almaty: Scientific and Production Center of Farming and Plant Growing; 2007. Available from: https://www.fao.org/4/i1500e/Kazakhstan.pdf [accessed Jan. 21, 2025].</mixed-citation><mixed-citation xml:lang="en">Country report on the state of plant genetic resources for food and agriculture in the Kazakhstan Republic. Almaty: Scientific and Production Center of Farming and Plant Growing; 2007. Available from: https://www.fao.org/4/i1500e/Kazakhstan.pdf [accessed Jan. 21, 2025].</mixed-citation></citation-alternatives></ref><ref id="cit5"><label>5</label><citation-alternatives><mixed-citation xml:lang="ru">FAOSTAT. Food and Agriculture Organization of the United Nations: [website]. Available from: https://www.fao.org/home/en [accessed Jan. 21, 2025].</mixed-citation><mixed-citation xml:lang="en">FAOSTAT. Food and Agriculture Organization of the United Nations: [website]. Available from: https://www.fao.org/home/en [accessed Jan. 21, 2025].</mixed-citation></citation-alternatives></ref><ref id="cit6"><label>6</label><citation-alternatives><mixed-citation xml:lang="ru">Hogg R. The British pomology: the history, description, classification, and synonymes, of the fruits and fruit trees of Great Britain. Cambridge: Cambridge University Press; 2012. DOI: 10.1017/CBO9781139108270</mixed-citation><mixed-citation xml:lang="en">Hogg R. The British pomology: the history, description, classification, and synonymes, of the fruits and fruit trees of Great Britain. Cambridge: Cambridge University Press; 2012. DOI: 10.1017/CBO9781139108270</mixed-citation></citation-alternatives></ref><ref id="cit7"><label>7</label><citation-alternatives><mixed-citation xml:lang="ru">Kheloufi A., Mansouri L.M., Vanbellinghen C. Seed germination of Crataegus monogyna – a species with a stony endocarp. Reforesta. 2019;7(7):73-80. DOI: 10.21750/REFOR.7.06.68</mixed-citation><mixed-citation xml:lang="en">Kheloufi A., Mansouri L.M., Vanbellinghen C. Seed germination of Crataegus monogyna – a species with a stony endocarp. Reforesta. 2019;7(7):73-80. DOI: 10.21750/REFOR.7.06.68</mixed-citation></citation-alternatives></ref><ref id="cit8"><label>8</label><citation-alternatives><mixed-citation xml:lang="ru">Kim H.H., Popova E., Shin D.J., Yi J.Y., Kim C.H., Yoon M.K. et al. Cryobanking of Korean Allium germplasm collections: Results from a 10 year experience. CryoLetters. 2012;33(1):45-57.</mixed-citation><mixed-citation xml:lang="en">Kim H.H., Popova E., Shin D.J., Yi J.Y., Kim C.H., Yoon M.K. et al. Cryobanking of Korean Allium germplasm collections: Results from a 10 year experience. CryoLetters. 2012;33(1):45-57.</mixed-citation></citation-alternatives></ref><ref id="cit9"><label>9</label><citation-alternatives><mixed-citation xml:lang="ru">Knop W. Quantitative Utersuchungen Über den ErnährungensprozeB der Pflanze. Landwirtschaftliche Versuchsstation. 1865;7:93-107. [in German]</mixed-citation><mixed-citation xml:lang="en">Knop W. Quantitative Utersuchungen Über den ErnährungensprozeB der Pflanze. Landwirtschaftliche Versuchsstation. 1865;7:93-107. [in German]</mixed-citation></citation-alternatives></ref><ref id="cit10"><label>10</label><citation-alternatives><mixed-citation xml:lang="ru">Kovalchuk I., Zhumagulova Z., Turdiev T., Reed B.M. Growth medium alterations improve in vitro cold storage of pear germplasm. CryoLetters. 2014;35(3):197-203.</mixed-citation><mixed-citation xml:lang="en">Kovalchuk I., Zhumagulova Z., Turdiev T., Reed B.M. Growth medium alterations improve in vitro cold storage of pear germplasm. CryoLetters. 2014;35(3):197-203.</mixed-citation></citation-alternatives></ref><ref id="cit11"><label>11</label><citation-alternatives><mixed-citation xml:lang="ru">Kumar N., Reddy M.P. In vitro plant propagation: A review. Journal of Forest and Environmental Science. 2011;27(2):61-72. DOI: 10.7747/JFS.2011.27.2.1</mixed-citation><mixed-citation xml:lang="en">Kumar N., Reddy M.P. In vitro plant propagation: A review. Journal of Forest and Environmental Science. 2011;27(2):61-72. DOI: 10.7747/JFS.2011.27.2.1</mixed-citation></citation-alternatives></ref><ref id="cit12"><label>12</label><citation-alternatives><mixed-citation xml:lang="ru">Kushnarenko S.V., Romadanova N.V., Aralbayeva M.M. Current state and in vitro conservation of the only endangered population of Corylus avellana in Kazakhstan. Research on Crops. 2020;21(4):681-686. DOI: 10.31830/2348-7542.2020.106</mixed-citation><mixed-citation xml:lang="en">Kushnarenko S.V., Romadanova N.V., Aralbayeva M.M. Current state and in vitro conservation of the only endangered population of Corylus avellana in Kazakhstan. Research on Crops. 2020;21(4):681-686. DOI: 10.31830/2348-7542.2020.106</mixed-citation></citation-alternatives></ref><ref id="cit13"><label>13</label><citation-alternatives><mixed-citation xml:lang="ru">Kushnarenko S.V., Rymkhanova N.K, Aralbayeva M.M., Romadanova N.V. In vitro cold acclimation is required for successful cryopreservation of Juglans regia L. shoot tips. CryoLetters. 2023;44(4):240-248. DOI: 10.54680/fr23410110612</mixed-citation><mixed-citation xml:lang="en">Kushnarenko S.V., Rymkhanova N.K, Aralbayeva M.M., Romadanova N.V. In vitro cold acclimation is required for successful cryopreservation of Juglans regia L. shoot tips. CryoLetters. 2023;44(4):240-248. DOI: 10.54680/fr23410110612</mixed-citation></citation-alternatives></ref><ref id="cit14"><label>14</label><citation-alternatives><mixed-citation xml:lang="ru">Lateur M., Dapena E., Szalatnay D., Gantar M.E., Guyader A., Hjalmarsson I., Höfer M., Ikase L., Kellerhals M., Lacis G., Militaru M., Miranda Jiménez C., Osterc G., Rondia A., Volens K., Zeljković M.K., Ordidge M. ECPGR characterization and evaluation descriptors for apple genetic resources. Apple (Malus × domestica). Rome: ECPGR; 2022. Available from: https://www.ecpgr.org/fileadmin/bioversity/publications/pdfs/2022_ECPGR_Malus_descriptors_final.pdf [accessed Jan. 21, 2025].</mixed-citation><mixed-citation xml:lang="en">Lateur M., Dapena E., Szalatnay D., Gantar M.E., Guyader A., Hjalmarsson I., Höfer M., Ikase L., Kellerhals M., Lacis G., Militaru M., Miranda Jiménez C., Osterc G., Rondia A., Volens K., Zeljković M.K., Ordidge M. ECPGR characterization and evaluation descriptors for apple genetic resources. Apple (Malus × domestica). Rome: ECPGR; 2022. Available from: https://www.ecpgr.org/fileadmin/bioversity/publications/pdfs/2022_ECPGR_Malus_descriptors_final.pdf [accessed Jan. 21, 2025].</mixed-citation></citation-alternatives></ref><ref id="cit15"><label>15</label><citation-alternatives><mixed-citation xml:lang="ru">Mammadov T., Abbaszade Z. Rare endangered species of the Rosaceae family of the Rosa L. genus in the flora of Azerbaijan. Bulletin of Science and Practice. 2022;8(4):51-57. DOI: 10.33619/2414-2948/77/05</mixed-citation><mixed-citation xml:lang="en">Mammadov T., Abbaszade Z. Rare endangered species of the Rosaceae family of the Rosa L. genus in the flora of Azerbaijan. Bulletin of Science and Practice. 2022;8(4):51-57. DOI: 10.33619/2414-2948/77/05</mixed-citation></citation-alternatives></ref><ref id="cit16"><label>16</label><citation-alternatives><mixed-citation xml:lang="ru">Marchese C. Biodiversity hotspots: A shortcut for a more complicated concept. Global Ecology and Conservation. 2015;3:297-309. DOI: 10.1016/j.gecco.2014.12.008</mixed-citation><mixed-citation xml:lang="en">Marchese C. Biodiversity hotspots: A shortcut for a more complicated concept. Global Ecology and Conservation. 2015;3:297-309. DOI: 10.1016/j.gecco.2014.12.008</mixed-citation></citation-alternatives></ref><ref id="cit17"><label>17</label><citation-alternatives><mixed-citation xml:lang="ru">Молканова О.И., Васильева О.Г., Коновалова Л.Н. Научные основы сохранения и устойчивого воспроизводства генофонда растений в культуре in vitro. Вестник Удмуртского университета. Серия Биология. Науки о Земле. 2015;25(2):95-100.</mixed-citation><mixed-citation xml:lang="en">Molkanova O.I., Vasilyeva O.G., Konovalova L.N. The scientific basis for conservation and sustainable reproduction of plant genofond in culture in vitro. Bulletin of Udmurt University. Series Biology. Earth Sciences. 2015;25(2):95-100. [in Russian]</mixed-citation></citation-alternatives></ref><ref id="cit18"><label>18</label><citation-alternatives><mixed-citation xml:lang="ru">Murashige T., Skoog F.A. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum. 1962;15(3):473-497. DOI: 10.1111/j.1399-3054.1962.tb08052.x</mixed-citation><mixed-citation xml:lang="en">Murashige T., Skoog F.A. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum. 1962;15(3):473-497. DOI: 10.1111/j.1399-3054.1962.tb08052.x</mixed-citation></citation-alternatives></ref><ref id="cit19"><label>19</label><citation-alternatives><mixed-citation xml:lang="ru">Myers N., Mittermeier R.A., Mittermeier C.G., da Fonseca G.A., Kent J. Biodiversity hotspots for conservation priorities. Nature. 2000;403(6772):853-858. DOI: 10.1038/35002501</mixed-citation><mixed-citation xml:lang="en">Myers N., Mittermeier R.A., Mittermeier C.G., da Fonseca G.A., Kent J. Biodiversity hotspots for conservation priorities. Nature. 2000;403(6772):853-858. DOI: 10.1038/35002501</mixed-citation></citation-alternatives></ref><ref id="cit20"><label>20</label><citation-alternatives><mixed-citation xml:lang="ru">Pasternak T.P., Steinmacher D. Plant growth regulation in cell and tissue culture in vitro. Plants (Basel). 2024;13(2):327. DOI: 10.3390/plants13020327</mixed-citation><mixed-citation xml:lang="en">Pasternak T.P., Steinmacher D. Plant growth regulation in cell and tissue culture in vitro. Plants (Basel). 2024;13(2):327. DOI: 10.3390/plants13020327</mixed-citation></citation-alternatives></ref><ref id="cit21"><label>21</label><citation-alternatives><mixed-citation xml:lang="ru">Petruzzello M. List of economically important members of the family Rosaceae. In: Britannica; 2024. Available from: https://www.britannica.com/plant/Rosaceae [accessed on Jan. 14, 2025].</mixed-citation><mixed-citation xml:lang="en">Petruzzello M. List of economically important members of the family Rosaceae. In: Britannica; 2024. Available from: https://www.britannica.com/plant/Rosaceae [accessed on Jan. 14, 2025].</mixed-citation></citation-alternatives></ref><ref id="cit22"><label>22</label><citation-alternatives><mixed-citation xml:lang="ru">Popov A.S., Popova E.V., Nikishina T.V., Vysotskaya O.N. Cryobank of plant genetic resources in Russian Academy of Sciences. International Journal of Refrigeration. 2006;29(3):403-410. DOI: 10.1016/j.ijrefrig.2005.07.011</mixed-citation><mixed-citation xml:lang="en">Popov A.S., Popova E.V., Nikishina T.V., Vysotskaya O.N. Cryobank of plant genetic resources in Russian Academy of Sciences. International Journal of Refrigeration. 2006;29(3):403-410. DOI: 10.1016/j.ijrefrig.2005.07.011</mixed-citation></citation-alternatives></ref><ref id="cit23"><label>23</label><citation-alternatives><mixed-citation xml:lang="ru">Красная книга Казахстана. Т. 2. Растения. 2-е изд. Астана: АртPrintXXI; 2014.</mixed-citation><mixed-citation xml:lang="en">Red Data Book of Kazakhstan. Vol. 2. Plants. 2nd ed. Astana: ArtPrintXXI; 2014. [in Russian]</mixed-citation></citation-alternatives></ref><ref id="cit24"><label>24</label><citation-alternatives><mixed-citation xml:lang="ru">Reed B.M. (ed.). Plant cryopreservation: A practical guide. New York, NY: Springer Verlag; 2008. DOI: 10.1007/978-0-387-72276-4</mixed-citation><mixed-citation xml:lang="en">Reed B.M. (ed.). Plant cryopreservation: A practical guide. New York, NY: Springer Verlag; 2008. DOI: 10.1007/978-0-387-72276-4</mixed-citation></citation-alternatives></ref><ref id="cit25"><label>25</label><citation-alternatives><mixed-citation xml:lang="ru">Romadanova N.V., Aralbayeva M.M., Zemtsova A.S., Alexandrova A.M., Kazybayeva S.Zh., Mikhailenko N.V. et al. In vitro collection for the safe storage of grapevine hybrids and identification of the presence of Plasmopora viticola resistance genes. Plants. 2024;13(8):1089. DOI: 10.3390/plants13081089</mixed-citation><mixed-citation xml:lang="en">Romadanova N.V., Aralbayeva M.M., Zemtsova A.S., Alexandrova A.M., Kazybayeva S.Zh., Mikhailenko N.V. et al. In vitro collection for the safe storage of grapevine hybrids and identification of the presence of Plasmopora viticola resistance genes. Plants. 2024;13(8):1089. DOI: 10.3390/plants13081089</mixed-citation></citation-alternatives></ref><ref id="cit26"><label>26</label><citation-alternatives><mixed-citation xml:lang="ru">Ромаданова Н.В., Кушнаренко С.В. Сохранение биоразнообразия растений методами биотехнологии. Труды по прикладной ботанике, генетике и селекции. 2023;184(1):239-248. DOI: 10.30901/2227-8834-2023-1-239-248</mixed-citation><mixed-citation xml:lang="en">Romadanova N.V., Kushnarenko S.V. Conservation of plant biodiversity by biotechnology methods. Proceedings on Applied Botany, Genetics and Breeding. 2023;184(1):239-248. [in Russian] 10.30901/2227-8834-2023-1-239-248</mixed-citation></citation-alternatives></ref><ref id="cit27"><label>27</label><citation-alternatives><mixed-citation xml:lang="ru">Romadanova N.V., Mishustina S.A., Matakova G.N., Kushnarenko S.V., Rakhimbaev I.R., Reed B.M. In vitro collection of Malus shoot cultures for cryogenic bank development in Kazakhstan. Acta Horticulturae. 2016;1113:271-277. DOI: 10.17660/ActaHortic.2016.1113.40</mixed-citation><mixed-citation xml:lang="en">Romadanova N.V., Mishustina S.A., Matakova G.N., Kushnarenko S.V., Rakhimbaev I.R., Reed B.M. In vitro collection of Malus shoot cultures for cryogenic bank development in Kazakhstan. Acta Horticulturae. 2016;1113:271-277. DOI: 10.17660/ActaHortic.2016.1113.40</mixed-citation></citation-alternatives></ref><ref id="cit28"><label>28</label><citation-alternatives><mixed-citation xml:lang="ru">Shaik S.P., Thomas P. In vitro activation of seed-transmitted cultivation-recalcitrant endophytic bacteria in tomato and host–endophyte mutualism. Microorganisms. 2019;7(5):132. DOI: 10.3390/microorganisms7050132</mixed-citation><mixed-citation xml:lang="en">Shaik S.P., Thomas P. In vitro activation of seed-transmitted cultivation-recalcitrant endophytic bacteria in tomato and host–endophyte mutualism. Microorganisms. 2019;7(5):132. DOI: 10.3390/microorganisms7050132</mixed-citation></citation-alternatives></ref><ref id="cit29"><label>29</label><citation-alternatives><mixed-citation xml:lang="ru">SYSTAT 13.0.5: [website]. Available from: https://systat.informer.com/13.0 [accessed Jan. 21, 2025].</mixed-citation><mixed-citation xml:lang="en">SYSTAT 13.0.5: [website]. Available from: https://systat.informer.com/13.0 [accessed Jan. 21, 2025].</mixed-citation></citation-alternatives></ref><ref id="cit30"><label>30</label><citation-alternatives><mixed-citation xml:lang="ru">Vinoth A., Ravindhran R. In vitro propagation – a potential method for plant conservation. International Journal of Computing Algorithm. 2013;2:268-272.</mixed-citation><mixed-citation xml:lang="en">Vinoth A., Ravindhran R. In vitro propagation – a potential method for plant conservation. International Journal of Computing Algorithm. 2013;2:268-272.</mixed-citation></citation-alternatives></ref><ref id="cit31"><label>31</label><citation-alternatives><mixed-citation xml:lang="ru">Viss P.R., Brooks E.M., Driver J.A. A simplified method for the control of bacterial contamination in woody plant tissue culture. In Vitro Cellular and Developmental Biology – Plant. 1991;27(1):42. DOI: 10.1007/BF02632060</mixed-citation><mixed-citation xml:lang="en">Viss P.R., Brooks E.M., Driver J.A. A simplified method for the control of bacterial contamination in woody plant tissue culture. In Vitro Cellular and Developmental Biology – Plant. 1991;27(1):42. DOI: 10.1007/BF02632060</mixed-citation></citation-alternatives></ref></ref-list><fn-group><fn fn-type="conflict"><p>The authors declare that there are no conflicts of interest present.</p></fn></fn-group></back></article>
