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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">vir-nw</journal-id><journal-title-group><journal-title xml:lang="ru">Труды по прикладной ботанике, генетике и селекции</journal-title><trans-title-group xml:lang="en"><trans-title>Proceedings on applied botany, genetics and breeding</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2227-8834</issn><issn pub-type="epub">2619-0982</issn><publisher><publisher-name>N.I. Vavilov All-Russian Institute of Plant Genetic Resources</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.30901/2227-8834-2025-1-61-70</article-id><article-id custom-type="elpub" pub-id-type="custom">vir-nw-2250</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ИЗУЧЕНИЕ И ИСПОЛЬЗОВАНИЕ ГЕНЕТИЧЕСКИХ РЕСУРСОВ РАСТЕНИЙ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>STUDYING AND UTILIZATION OF PLANT GENETIC RESOURCES</subject></subj-group></article-categories><title-group><article-title>Изучение качества семян русского одуванчика</article-title><trans-title-group xml:lang="en"><trans-title>Study on seed quality testing of Russian dandelion</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-5320-6465</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Шень</surname><given-names>Г.</given-names></name><name name-style="western" xml:lang="en"><surname>Shen</surname><given-names>G.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Гуан Шен, доктор философии, Институт природных ресурсов и экологии Академии наук провинции Хэйлунцзян</p><p>провинция Хэйлунцзян, Харбин, район Сянфан, ул. Хапин, 103</p></bio><bio xml:lang="en"><p>Guang Shen, Ph. D.</p><p>103 Haping Road, Xiangfang District, Harbin, Heilongjiang Province</p></bio><email xlink:type="simple">shen19772@163.com</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-9695-417X</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Чжоу</surname><given-names>Л.</given-names></name><name name-style="western" xml:lang="en"><surname>Zhou</surname><given-names>L.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Линь Чжоу, магистр, младший научный сотрудник</p><p>провинция Хэйлунцзян, Харбин, район Сянфан, ул. Хапин, 103</p></bio><bio xml:lang="en"><p>Lin Zhou, M. Sc., Associate Researcher</p><p>103 Haping Road, Xiangfang District, Harbin, Heilongjiang Province</p></bio><email xlink:type="simple">1016393991@qq.com</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-8966-5230</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Чжэн</surname><given-names>Ф.</given-names></name><name name-style="western" xml:lang="en"><surname>Zheng</surname><given-names>F.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Фуюн Чжэн, магистр</p><p>провинция Хэйлунцзян, Харбин, район Сянфан, ул. Хапин, 103</p></bio><bio xml:lang="en"><p>Fuyun Zheng, M. Sc.</p><p>103 Haping Road, Xiangfang District, Harbin, Heilongjiang Province</p></bio><email xlink:type="simple">zhengfuyun@163.com</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0009-0009-2867-860X</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Юй</surname><given-names>Ч.</given-names></name><name name-style="western" xml:lang="en"><surname>Yu</surname><given-names>Zh.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Чжан Юй, магистр</p><p>провинция Хэйлунцзян, Харбин, район Сянфан, ул. Хэсин, 26</p></bio><bio xml:lang="en"><p>Zhang Yu, M. Sc.</p><p>26 Hexing Road, Xiangfang District, Harbin, Heilongjiang Province</p></bio><email xlink:type="simple">87364962@qq.com</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0009-0008-6644-6060</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Ян</surname><given-names>И.</given-names></name><name name-style="western" xml:lang="en"><surname>Yang</surname><given-names>Y.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Ихуа Ян, магистр</p><p>провинция Хэйлунцзян, Харбин, район Сянфан, ул. Хапин, 103</p></bio><bio xml:lang="en"><p>Yihua Yang, M. Sc.</p><p>103 Haping Road, Xiangfang District, Harbin, Heilongjiang Province</p></bio><email xlink:type="simple">yyh_1975@163.com</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-6877-3518</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Чжан</surname><given-names>В.</given-names></name><name name-style="western" xml:lang="en"><surname>Zhang</surname><given-names>W.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Вэньтянь Чжан, магистр</p><p>провинция Хэйлунцзян, Харбин, район Сянфан, ул. Хапин, 103</p></bio><bio xml:lang="en"><p>Wentian Zhang, M. Sc.</p><p>103 Haping Road, Xiangfang District, Harbin, Heilongjiang Province</p></bio><email xlink:type="simple">swxzwt@126.com</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-4920-3904</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Конькова</surname><given-names>Н. Г.</given-names></name><name name-style="western" xml:lang="en"><surname>Kon’kova</surname><given-names>N. G.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Нина Григорьевна Конькова, научный сотрудник</p><p>Санкт-Петербург, ул. Б. Морская, 42, 44</p></bio><bio xml:lang="en"><p>Nina G. Kon’kova, Researcher</p><p>St. Petersburg 190000</p></bio><email xlink:type="simple">n.konkova@vir.nw.ru</email><xref ref-type="aff" rid="aff-3"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>Институт природных ресурсов и экологии Академии наук провинции Хэйлунцзян</institution><country>Китай</country></aff><aff xml:lang="en"><institution>Institute of Natural Resources and Ecology, Heilongjiang Academy of Sciences</institution><country>China</country></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru"><institution>Северо-Восточный лесотехнический университет, Колледж ландшафтной архитектуры</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Northeast Forestry University, College of Landscape Architecture</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-3"><aff xml:lang="ru"><institution>Федеральный исследовательский центр Всероссийский институт генетических ресурсов растений имени Н.И. Вавилова</institution><country>Россия</country></aff><aff xml:lang="en"><institution>N.I. Vavilov All-Russian Institute of Plant Genetic Resources</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2025</year></pub-date><pub-date pub-type="epub"><day>11</day><month>04</month><year>2025</year></pub-date><volume>186</volume><issue>1</issue><fpage>61</fpage><lpage>70</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Шень Г., Чжоу Л., Чжэн Ф., Юй Ч., Ян И., Чжан В., Конькова Н.Г., 2025</copyright-statement><copyright-year>2025</copyright-year><copyright-holder xml:lang="ru">Шень Г., Чжоу Л., Чжэн Ф., Юй Ч., Ян И., Чжан В., Конькова Н.Г.</copyright-holder><copyright-holder xml:lang="en">Shen G., Zhou L., Zheng F., Yu Z., Yang Y., Zhang W., Kon’kova N.G.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://elpub.vir.nw.ru/jour/article/view/2250">https://elpub.vir.nw.ru/jour/article/view/2250</self-uri><abstract><sec><title>Актуальность</title><p>Актуальность. Кок-сагыз, или русский одуванчик, входит в тройку самых перспективных каучуконосов в мире. В этом исследовании изучалось качество семян кок-сагыза из разных источников со ссылкой на методы, изложенные в Международных правилах тестирования семян.</p></sec><sec><title>Материалы и методы</title><p>Материалы и методы. Объектом исследования служили образец кок-сагыза из коллекции ВИР (к-445) и образец американского происхождения. Исследования проводили в 2018–2020 гг. в условиях полевого опыта экспериментальной станции в Харбине (45.592729°с. ш., 126.581668°в. д.).</p></sec><sec><title>Результаты</title><p>Результаты. В ходе исследования определили принципы и методы отбора проб, а также провели всестороннюю оценку качества семян кок-сагыза. Анализ чистоты показал, что чистота семян обоих образцов кок-сагыза превышает 80%. Вес 1000 семян равен примерно 0,5 г, определялся с помощью метода 100 семян. Содержание влаги, измеренное методом низкотемпературной сушки, составляет около 5,4%. Время, необходимое для достижения естественной скорости насыщения семян влагой, составило 2,5 ч. Проверка на подлинность показала, что семена кок-сагыза можно отличить от других видов рода Taraxacum по внешним характеристикам. В ходе проверки на грибковые заболевания обнаружили четыре типа плесени внутри и снаружи семян кок-сагыза.</p></sec><sec><title>Заключение</title><p>Заключение. Данное исследование обеспечивает техническую поддержку технологии оценки качества семян коксагыза, а также предоставляет рекомендации по оценке материала для последующей коммерческой реализации семян кок-сагыза.</p></sec></abstract><trans-abstract xml:lang="en"><sec><title>Background</title><p>Background. Kok-saghyz, or Russian dandelion, is one of the three most promising natural rubber plants in the world. This study examined the seed quality of kok-saghyz from different sources with reference to the methods in the International Rules for Seed Testing.</p></sec><sec><title>Materials and methods</title><p>Materials and methods. A kok-saghyz accession from the VIR collection (k-445) and an accession of American origin were analyzed. The study was carried out in 2018–2020 under the conditions of a field experiment at the experiment station in Harbin (45.592729°N, 126.581668°E).</p></sec><sec><title>Results</title><p>Results. Sampling principles and methods were established, and the seed quality of kok-saghyz was thoroughly evaluated. A seed purity test showed above 80% of pure seeds for both accessions. The thousand-seed weight was approximately 0.5 g when measured by the hundred-seed method. The moisture content was about 5.4% according to the low-temperature drying technique. The time required for reaching a natural imbibition rate was 2.5 h. The authenticity test demonstrated that koksaghyz seeds can be distinguished from those of other Taraxacum spp. according to their appearance. The health test found 4 types of mold inside and outside the kok-saghyz seeds.</p></sec><sec><title>Conclusion</title><p>Conclusion. This study provides technical support for kok-saghyz seed quality testing, and offers recommendations for koksaghyz seed trade.</p></sec></trans-abstract><kwd-group xml:lang="ru"><kwd>каучуконосное растение</kwd><kwd>кок-сагыз</kwd><kwd>качество семян</kwd><kwd>правила проведения испытаний</kwd></kwd-group><kwd-group xml:lang="en"><kwd>rubber plant</kwd><kwd>kok-saghyz</kwd><kwd>seed quality</kwd><kwd>seed testing regulations</kwd></kwd-group><funding-group><funding-statement xml:lang="ru">Работа поддержана Фондом научных исследований Академии наук провинции Хэйлунцзян в рамках грантов № KY2023ZR04 и № KY2018ZR02; выполнялась при поддержке проекта «Текущие расходы на исследования» Научно-исследовательского института провинции Хэйлунцзян (грант № ZNBZ2022ZR04) и в рамках государственного задания согласно тематическому плану ВИР по проекту № FGEM-2022-0005.</funding-statement><funding-statement xml:lang="en">The work was supported by the Scientific Research Fund of Heilongjiang Academy of Sciences under Grants Nos. KY2019ZR02 and KY2018ZR02, and performed with support from the Research Operating Expense Project of the Research Institute of Heilongjiang Province, Grant No. ZNBZ2022ZR04, and within the framework of the state task according to the theme plan of VIR, Project No. FGEM-2022-0005.</funding-statement></funding-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Dan H.M. Studies on rules for seed testing and standards for quality grading of Glycyrrhiza uralensis, Salvia miltiorrhiza and Achyranthes bidentata seeds [dissertation]. Beijing: Peking Union Medical College; 2006. [in Chinese]</mixed-citation><mixed-citation xml:lang="en">Dan H.M. Studies on rules for seed testing and standards for quality grading of Glycyrrhiza uralensis, Salvia miltiorrhiza and Achyranthes bidentata seeds [dissertation]. Beijing: Peking Union Medical College; 2006. [in Chinese]</mixed-citation></citation-alternatives></ref><ref id="cit2"><label>2</label><citation-alternatives><mixed-citation xml:lang="ru">Hu J. Seed testing science. Beijing: Science Press; 2015. [in Chinese]</mixed-citation><mixed-citation xml:lang="en">Hu J. Seed testing science. Beijing: Science Press; 2015. [in Chinese]</mixed-citation></citation-alternatives></ref><ref id="cit3"><label>3</label><citation-alternatives><mixed-citation xml:lang="ru">ISTA. International Rules for Seed Testing. Zurich: International Seed Testing Association (ISTA); 1996.</mixed-citation><mixed-citation xml:lang="en">ISTA. International Rules for Seed Testing. Zurich: International Seed Testing Association (ISTA); 1996.</mixed-citation></citation-alternatives></ref><ref id="cit4"><label>4</label><citation-alternatives><mixed-citation xml:lang="ru">ISTA. International Rules for Seed Testing. Zurich: International Seed Testing Association (ISTA); 2020.</mixed-citation><mixed-citation xml:lang="en">ISTA. International Rules for Seed Testing. Zurich: International Seed Testing Association (ISTA); 2020.</mixed-citation></citation-alternatives></ref><ref id="cit5"><label>5</label><citation-alternatives><mixed-citation xml:lang="ru">Luo S. Russian dandelion. Beijing: Chinese Academy of Sciences Press; 1951.</mixed-citation><mixed-citation xml:lang="en">Luo S. Russian dandelion. Beijing: Chinese Academy of Sciences Press; 1951.</mixed-citation></citation-alternatives></ref><ref id="cit6"><label>6</label><citation-alternatives><mixed-citation xml:lang="ru">Molinu M.G., Piluzza G., Campesi G., Sulas L., Re G. Antioxidant sources from leaves of Russian dandelion. Chemistry and Biodiversity. 2019;16(8):e1900250. DOI: 10.1002/cbdv.201900250</mixed-citation><mixed-citation xml:lang="en">Molinu M.G., Piluzza G., Campesi G., Sulas L., Re G. Antioxidant sources from leaves of Russian dandelion. Chemistry and Biodiversity. 2019;16(8):e1900250. DOI: 10.1002/cbdv.201900250</mixed-citation></citation-alternatives></ref><ref id="cit7"><label>7</label><citation-alternatives><mixed-citation xml:lang="ru">Polhamus L.G. Rubber: botany, production and utilization. London: Leonard Hill; 1962.</mixed-citation><mixed-citation xml:lang="en">Polhamus L.G. Rubber: botany, production and utilization. London: Leonard Hill; 1962.</mixed-citation></citation-alternatives></ref><ref id="cit8"><label>8</label><citation-alternatives><mixed-citation xml:lang="ru">Shen G. Efficient propagation, field management, and cultivation mode optimization for a rubber-producing grass of Russian dandelion. Harbin: Northeast Forestry University; 2021. [in Chinese] DOI: 10.27009/d.cnki.gdblu.2021.000022</mixed-citation><mixed-citation xml:lang="en">Shen G. Efficient propagation, field management, and cultivation mode optimization for a rubber-producing grass of Russian dandelion. Harbin: Northeast Forestry University; 2021. [in Chinese] DOI: 10.27009/d.cnki.gdblu.2021.000022</mixed-citation></citation-alternatives></ref><ref id="cit9"><label>9</label><citation-alternatives><mixed-citation xml:lang="ru">Shen G., Chen F., Wang W., Zheng F., Wei D., Li L. et al. Methodological study on selection of conditions for improving Taraxacum kok-saghyz Rodin seed germination. Agricultural Biology. 2019;54(3):548-556. DOI: 10.15389/agrobiology.2019.3.548eng</mixed-citation><mixed-citation xml:lang="en">Shen G., Chen F., Wang W., Zheng F., Wei D., Li L. et al. Methodological study on selection of conditions for improving Taraxacum kok-saghyz Rodin seed germination. Agricultural Biology. 2019;54(3):548-556. DOI: 10.15389/agrobiology.2019.3.548eng</mixed-citation></citation-alternatives></ref><ref id="cit10"><label>10</label><citation-alternatives><mixed-citation xml:lang="ru">Shen G., Wang W., Chen F., Zheng F., Wei D., Li L. et al. Nondestructive leaf area and fresh weight estimation for Taraxacum kok-saghyz Rodin and their sampling number. Agricultural Biology. 2018;53(3):645-654. DOI: 10.15389/agrobiology.2018.3.645eng</mixed-citation><mixed-citation xml:lang="en">Shen G., Wang W., Chen F., Zheng F., Wei D., Li L. et al. Nondestructive leaf area and fresh weight estimation for Taraxacum kok-saghyz Rodin and their sampling number. Agricultural Biology. 2018;53(3):645-654. DOI: 10.15389/agrobiology.2018.3.645eng</mixed-citation></citation-alternatives></ref><ref id="cit11"><label>11</label><citation-alternatives><mixed-citation xml:lang="ru">Shen G., Zheng F., Zhou L., Zeng X., Konkova N.G. Effects of irrigation frequency on growth and yields of Taraxacum koksaghyz Rodin. Proceedings on Applied Botany, Genetics and Breeding. 2023;184(4):53-63. DOI: 10.30901/2227-8834-2023-4-53-63</mixed-citation><mixed-citation xml:lang="en">Shen G., Zheng F., Zhou L., Zeng X., Konkova N.G. Effects of irrigation frequency on growth and yields of Taraxacum koksaghyz Rodin. Proceedings on Applied Botany, Genetics and Breeding. 2023;184(4):53-63. DOI: 10.30901/2227-8834-2023-4-53-63</mixed-citation></citation-alternatives></ref><ref id="cit12"><label>12</label><citation-alternatives><mixed-citation xml:lang="ru">Whaley W.G., Bowen J.S. Russian dandelion (kok-saghyz): an emergency source of natural rubber. Washington DC: USA Department of Agriculture; 1947.</mixed-citation><mixed-citation xml:lang="en">Whaley W.G., Bowen J.S. Russian dandelion (kok-saghyz): an emergency source of natural rubber. Washington DC: USA Department of Agriculture; 1947.</mixed-citation></citation-alternatives></ref><ref id="cit13"><label>13</label><citation-alternatives><mixed-citation xml:lang="ru">Wu J., Zhao X., Ning W. Micro-morphological characteristics of Taraxacum F.H. Wigg. seeds from Northeastern China and taxonomic significance. Chinese Bulletin of Botany. 2011;46(4):437-446. DOI: 10.3724/SP.J.1259.2011.00437</mixed-citation><mixed-citation xml:lang="en">Wu J., Zhao X., Ning W. Micro-morphological characteristics of Taraxacum F.H. Wigg. seeds from Northeastern China and taxonomic significance. Chinese Bulletin of Botany. 2011;46(4):437-446. DOI: 10.3724/SP.J.1259.2011.00437</mixed-citation></citation-alternatives></ref><ref id="cit14"><label>14</label><citation-alternatives><mixed-citation xml:lang="ru">Wu Y., Dong G., Luo F., Xie H., Li X., Yan J. TkJAZs-TkMYC2-TkSRPP/REF regulates the biosynthesis of natural rubber in Taraxacum kok-saghyz. Plants (Basel). 2024;13(15):2034. DOI: 10.3390/plants13152034</mixed-citation><mixed-citation xml:lang="en">Wu Y., Dong G., Luo F., Xie H., Li X., Yan J. TkJAZs-TkMYC2-TkSRPP/REF regulates the biosynthesis of natural rubber in Taraxacum kok-saghyz. Plants (Basel). 2024;13(15):2034. DOI: 10.3390/plants13152034</mixed-citation></citation-alternatives></ref><ref id="cit15"><label>15</label><citation-alternatives><mixed-citation xml:lang="ru">Zhang W., Zheng F., Wei D., Shen G., Zeng X., Kon’kova N.G. Effects of nitrogen, phosphorus, and potassium base fertilizers on growth and yield of Taraxacum kok-saghyz Rodin. Proceedings on Applied Botany, Genetics and Breeding. 2023;184(1):7078. DOI: 10.30901/2227-8834-2023-1-70-78</mixed-citation><mixed-citation xml:lang="en">Zhang W., Zheng F., Wei D., Shen G., Zeng X., Kon’kova N.G. Effects of nitrogen, phosphorus, and potassium base fertilizers on growth and yield of Taraxacum kok-saghyz Rodin. Proceedings on Applied Botany, Genetics and Breeding. 2023;184(1):7078. DOI: 10.30901/2227-8834-2023-1-70-78</mixed-citation></citation-alternatives></ref></ref-list><fn-group><fn fn-type="conflict"><p>The authors declare that there are no conflicts of interest present.</p></fn></fn-group></back></article>
