vir-nwТруды по прикладной ботанике, генетике и селекцииProceedings on applied botany, genetics and breeding2227-88342619-0982N.I. Vavilov All-Russian Institute of Plant Genetic Resources10.30901/2227-8834-2024-4-252-263vir-nw-2132Research ArticleОБЗОРЫSURVEYSNGS-секвенирование в селекционно-генетических исследованиях соиNext-generation sequencing in soybean breeding and genetic researchhttps://orcid.org/0009-0005-7915-441XМеньковМ. Т.MenkovM. T.

Михаил Тимофеевич Меньков младший научный сотрудник, Научно-технологический университет «Сириус», Научный центр генетики и наук о жизни, ФИЦ Всероссийский институт генетических ресурсов растений имени Н.И. Вавилова.

354340 Краснодарский край, федеральная территория «Сириус», пгт. Сириус, Олимпийский пр., 1; 190000 Санкт-Петербург, ул. Б. Морская, 42, 44

Mikhail T. Menkov Associate Researcher, Sirius Universit y of Science and Technology, Research Center of Genetics and Life Sciences, , N.I. Vavilov All-Russian Institute of Plant Genetic Resources,

1 Olimpiysky Ave., Sirius Settlem., Sirius Federal Territory, Krasnodar Territory 354340; 42, 44 Bolshaya Morskaya Street, St. Petersburg 190000

menkov.mt@talantiuspeh.ruhttps://orcid.org/0000-0003-4341-0766РозановаИ. В.RozanovaI. V.

Ирина Вениаминовна Розанова - кандидат биологических наук, старший научный сотрудник.

354340 Краснодарский край, федеральная территория «Сириус», пгт. Сириус, Олимпийский пр., 1

Irina V. Rozanova - Cand. Sci. (Biology), Senior Researcher.

1 Olimpiysky Ave., Sirius Settlem., Sirius Federal Territory, Krasnodar Territory 354340

i.rozanova@vir.nw.ruhttps://orcid.org/0000-0003-0246-8929ЕвлашА. Я.EvlashA. Ya.

Анастасия Ярославовна Евлаш младший научный сотрудник.

354340 Краснодарский край, федеральная территория «Сириус», пгт. Сириус, Олимпийский пр., 1

Anastasia Ya. Evlash - Associate Researcher.

1 Olimpiysky Ave., Sirius Settlem., Sirius Federal Territory, Krasnodar Territory 354340

evlash-nastja@rambler.ruhttps://orcid.org/0000-0002-8470-8254ХлесткинаЕ. К.KhlestkinaE. K.

Елена Константиновна Хлесткина - доктор биологических наук, профессор РАН, директор, ФИЦ Всероссийский институт генетических ресурсов растений имени Н.И. Вавилова, руководитель направления «Биология и биотехнология растений», НТУ «Сириус», Научный центр генетики и наук о жизни,

354340 Краснодарский край, федеральная территория «Сириус», пгт. Сириус, Олимпийский пр., 1; 190000 Санкт-Петербург, ул. Б. Морская, 42, 44

Elena K. Khlestkina - Dr. Sci. (Biology), Professor of the RAS, Director, N.I. Vavilov All-Russian Institute of Plant Genetic Resources, Plant Biology and Biotechnology Research Manager, Sirius University of Science and Technology, RC of Genetics and Life Sciences.

1 Olimpiysky Ave., Sirius Settlem., Sirius Federal Territory, Krasnodar Territory 354340; 42, 44 Bolshaya Morskaya Street, St. Petersburg 190000

director@vir.nw.ruНаучно-технологический университет «Сириус», Центр генетики и наук о жизни; Федеральный исследовательский центр Всероссийский институт генетических ресурсов растений имени Н.И. ВавиловаРоссияSirius University of Science and Technology, Research Center of Genetics and Life Sciences; N.I. Vavilov All-Russian Institute of Plant Genetic ResourcesRussian FederationНаучно-технологический университет «Сириус», Центр генетики и наук о жизниРоссияSirius University of Science and Technology, Research Center of Genetics and Life SciencesRussian Federation2024110120251854252263Copyright © Меньков М.Т., Розанова И.В., Евлаш А.Я., Хлесткина Е.К., 20242024Меньков М.Т., Розанова И.В., Евлаш А.Я., Хлесткина Е.К.Menkov M.T., Rozanova I.V., Evlash A.Y., Khlestkina E.K.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://elpub.vir.nw.ru/jour/article/view/2132

Соя (Glycine max (L.) Merr.) является одной из важнейших зернобобовых культур, производство которой растет с каждым годом и на 2024 г. составляет около 7 миллионов тонн. Целью данного обзора было обобщение последних достижений в селекции сои, в том числе с применением методов высокопроизводительного секвенирования и геномных технологий. Соя является одним из наиболее изучаемых растений. Ввиду ее важности для сельского хозяйства селекционеры постоянно реализуют в своей работе наиболее передовые методы. Исследования, выполненные в последние годы, показали преимущество подходов, основанных на применении молекулярно-генетических маркеров. Первый вариант последовательности генома сои, геном G. max ‘Williams 82’, представили в 2010 г., и это событие значимо ускорило изучение и развитие генетических исследований культуры. Полученные данные позволили создавать ресурсы, направленные как на изучение функциональной организации генов сои, так и на выведение новых улучшенных сортов. В обзоре обобщены результаты по крупным проектам секвенирования сои, в том числе и пангеномные работы. Описаны методы, применяющиеся для генетического картирования высокого разрешения, такие как анализ SNP с использованием чипов и метод GBS (genotyping-by-sequencing). Представлена информация о генах сои, связанных с хозяйственно ценными и селекционно значимыми признаками, идентификация которых позволила выделить их в качестве мишеней для редактирования.

Soybean (Glycine max (L.) Merr.) is one of the most important grain legume crops whose production has been growing every year and by 2024 reached ca. 7 million tons. The objective of this review was to summarize the latest achievements in soybean breeding, including the use of high-throughput sequencing methods and genomic technologies. Soybean is one of the most studied plants. The studies of recent years showed the advantage of approaches based on the use of molecular genetic markers in breeding. The first version of the soybean genome sequence, the G. max genome “Williams 82”, was presented in 2010, and this event significantly accelerated the study and development of genetic research on the crop. The data obtained made it possible to develop resources aimed at both studying the functional organization of soybean genes and breeding new improved cultivars. The review summarizes the results of large-scale soybean sequencing projects, including pan-genome works. Methods used for high-resolution genetic mapping, such as the SNP array analysis and the GBS (genotyping-by-sequencing) technique, are described. Information is provided on soybean genes associated with valuable agronomic and breeding-oriented traits whose identification made it possible to single them out as targets for editing.

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The authors declare that there are no conflicts of interest present.