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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">vir-nw</journal-id><journal-title-group><journal-title xml:lang="ru">Труды по прикладной ботанике, генетике и селекции</journal-title><trans-title-group xml:lang="en"><trans-title>Proceedings on applied botany, genetics and breeding</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2227-8834</issn><issn pub-type="epub">2619-0982</issn><publisher><publisher-name>N.I. Vavilov All-Russian Institute of Plant Genetic Resources</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.30901/2227-8834-2023-3-196-206</article-id><article-id custom-type="elpub" pub-id-type="custom">vir-nw-1666</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ИММУНИТЕТ КУЛЬТУРНЫХ РАСТЕНИЙ И ИХ ДИКИХ РОДИЧЕЙ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>IMMUNITY OF CULTIVATED PLANTS AND THEIR WILD RELATIVES</subject></subj-group></article-categories><title-group><article-title>Устойчивость сортов и линий яровой мягкой пшеницы к возбудителям септориозной, пиренофорозной и темно-бурой пятнистостей</article-title><trans-title-group xml:lang="en"><trans-title>Resistance of spring bread wheat cultivars and lines to Septoria leaf blotch, tan spot, and spot blotch pathogens</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-9716-288X</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Зеленева</surname><given-names>Ю. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Zeleneva</surname><given-names>Yu. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Юлия Витальевна Зеленева, доктор биологических наук, доцент, старший научный сотрудник</p><p>196608 Санкт-Петербург, Пушкин, ш. Подбельского, 3</p></bio><bio xml:lang="en"><p>Yulia V. Zeleneva, Dr. Sci. (Biology), Associate Professor, Senior Researcher</p><p>3 Podbelskogo Hwy., Pushkin, St. Petersburg 196608</p></bio><email xlink:type="simple">zelenewa@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-5367-1340</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Судникова</surname><given-names>В. Н.</given-names></name><name name-style="western" xml:lang="en"><surname>Sudnikovа</surname><given-names>V. P.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Валентина Павловна Судникова, кандидат сельскохозяйственных наук, старший научный сотрудник</p><p>392553 Тамбовская обл., Тамбовский р-н, пос. Новая Жизнь, ул. Молодежная, 1</p></bio><bio xml:lang="en"><p>Valentina P. Sudnikovа, Cand. Sci. (Agriculture), Senior Researcher</p><p>1 Molodezhnaya St., Novaya Zhizn Settlem., Tambovsky District, Tambov Province 392553</p></bio><email xlink:type="simple">sudnikova47@mail.ru</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-9577-8816</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Коваленко</surname><given-names>Н. М.</given-names></name><name name-style="western" xml:lang="en"><surname>Kovalenkо</surname><given-names>N. M.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Надежда Михайловна Коваленко, кандидат биологических наук, старший научный сотрудник</p><p>196608 Санкт-Петербург, Пушкин, ш. Подбельско-го, 3</p></bio><bio xml:lang="en"><p>Nadezhda М. Kovalenkо, Cand. Sci. (Biology), Senior Researcher</p><p>3 Podbelskogo Hwy., Pushkin, St. Petersburg 196608</p></bio><email xlink:type="simple">nadyakov@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-1063-4739</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Гусев</surname><given-names>И. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Gusev</surname><given-names>I. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Иван Викторович Гусев, старший научный сотрудник</p><p>392553 Тамбовская обл., Тамбовский р-н, пос. Новая Жизнь, ул. Молодежная, 1</p></bio><bio xml:lang="en"><p>Ivan V. Gusev, Senior Researcher</p><p>1 Molodezhnaya St., Novaya Zhizn Settlem., Tambovsky District, Tambov Province 392553</p></bio><email xlink:type="simple">tmbsnifs@mail.ru</email><xref ref-type="aff" rid="aff-2"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>Всероссийский научно-исследовательский институт защиты растений</institution><country>Россия</country></aff><aff xml:lang="en"><institution>All-Russian Research Institute of Plant Protection</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru"><institution>Федеральный научный центр имени И.В. Мичурина, Среднерусский филиал</institution><country>Россия</country></aff><aff xml:lang="en"><institution>I.V. Michurin Federal Science Center, Middle-Russian Branch</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2023</year></pub-date><pub-date pub-type="epub"><day>23</day><month>10</month><year>2023</year></pub-date><volume>184</volume><issue>3</issue><fpage>196</fpage><lpage>206</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Зеленева Ю.В., Судникова В.Н., Коваленко Н.М., Гусев И.В., 2023</copyright-statement><copyright-year>2023</copyright-year><copyright-holder xml:lang="ru">Зеленева Ю.В., Судникова В.Н., Коваленко Н.М., Гусев И.В.</copyright-holder><copyright-holder xml:lang="en">Zeleneva Y.V., Sudnikovа V.P., Kovalenkо N.M., Gusev I.V.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://elpub.vir.nw.ru/jour/article/view/1666">https://elpub.vir.nw.ru/jour/article/view/1666</self-uri><abstract><sec><title>Актуальность</title><p>Актуальность. Пятнистости зерновых культур – чрезвычайно вредоносные болезни в агроценозах Тамбовской области, существенно снижающие урожайность зерна. Цель работы – идентификация устойчивых к болезням сортов и линий яровой мягкой пшеницы, созданных в Среднерусском филиале ФНЦ имени И.В. Мичурина.</p></sec><sec><title>Материалы и методы</title><p>Материалы и методы. Материалом для исследования служили 3 сорта и 23 линии яровой мягкой пшеницы. Проростковую устойчивость образцов к болезням оценивали в лаборатории, взрослых растений – на естественном инфекционном фоне в 2020–2022 гг. С помощью молекулярного маркера Xfcp623 детектировали присутствие аллеля Tsn1, контролирующего чувствительность к токсину грибов PtrToxA.</p></sec><sec><title>Результаты и выводы</title><p>Результаты и выводы. Выделили образцы, обладающие высокой устойчивостью к Zymoseptoria tritici (Л-8252, Л-82/60, Л-8107, Л-8078(23), Л-43-9, Л-43-1, Л-4, Stb-8/15, Stb-7/15, Stb-92); Parastagonospora nodorum (Stb-97, Stb-89, Stb-89(a), Stb-90, Stb-34, Rl-6-22); P. pseudonodorum (Л-8134, Л-8114, Л-82/60, Л-8107, Stb-10/15, Stb-9/15, Stb-8/15, Stb7/15, Stb-97, Stb-89, Stb-89(a), Stb-90, Stb-34, Rl-6-22); Pyrenophora tritici-repentis (Л-8252, Л-8107, Л-43-9, Л-43-1, Л-4, Stb-10/15, Stb-9/15, Stb-97, Stb-92, Stb-92(a), Stb-89, Stb-89(a), Stb-34) и Bipolaris sorokiniana (Л-8114, Л-82/60, Л-43-9, Л-43-1, Л-4, Stb-90, Stb-34, Rl-6-22). Сорта и селекционные линии характеризовались групповой устойчивостью к двум (сорта ‘Тамбовчанка’, ‘Памяти Плахотника’; линии Л-8252; Stb-92, Л-8114, Stb-10/15; Stb-9/15, Stb-8/15; Stb-7/15), трем (линии Л-82/60, Л-8107, Л-43-9; Л-43-1; Л-4, Stb-97; Stb-89; Stb-89(a), Stb-90; Rl-6-22; сорт ‘Среднерусская’) и четырем (линия Stb-34) патогенам. Линии Л-8252, Л-8134, Л-82/60, Л-8107, Л-8078 (23), Л-43-9, Л-43-1, Л-4, Stb-89, Stb-90, Stb-34, Л-33809-7-3 несут рецессивный аллель tsn1 и следовательно защищены от токсина PtrToxA четырех грибов: Parastagonospora nodorum, P. pseudonodorum, Pyrenophora tritici-repentis и Bipolaris sorokiniana.</p></sec><sec><title> </title><p> </p></sec></abstract><trans-abstract xml:lang="en"><sec><title>Background</title><p>Background. Leaf and stem diseases of cereal crops are among the most harmful in the agrocenoses of Tambov Province, significantly reducing grain yield and rapidly progressing in many regions of Russia. The aim of the study was to identify spring bread wheat lines and cultivars developed at the Middle-Russian Branch of the I.V. Michurin Federal Science Center that show resistance to leaf and stem diseases.</p></sec><sec><title>Materials and methods</title><p>Materials and methods. The target material was represented by 3 cultivars and 23 breeding lines of spring bread wheat. Disease resistance in seedlings was assessed in the laboratory, while for adult plants the process was carried out in the field in 2020–2022. The presence of the Tsn1 allele controlling susceptibility to the PtrToxA fungal toxin was detected using the Xfcp623 molecular marker.</p></sec><sec><title>Results and conclusions</title><p>Results and conclusions. Accessions with high levels of resistance to Zymoseptoria tritici were identified: L-8252, L-82/60, L-8107, L-8078(23), L-43-9, L-43-1, L-4, Stb-8/15, Stb-7/15, and Stb-92. Accessions Stb-97, Stb-89, Stb-89(a), Stb-90, Stb-34, and Rl-6-22 were resistant to Parastagonospora nodorum; L-8134, L-8114, L-82/60, L-8107, Stb-10/15, Stb-9/15, Stb-8/15, Stb-7/15, Stb-97, Stb-89, Stb-89(a), Stb-90, Stb-34, and Rl-6-22 to P. pseudonodorum; L-8252, L-8107, L-43-9, L-43-1, L-4, Stb10/15, Stb-9/15, Stb-97, Stb-92, Stb-92(a), Stb-89, Stb-89(a), and Stb-34 to Pyrenophora tritici-repentis; and L-8114, L-82/60, L-43-9, L-43-1, L-4, Stb-90, Stb-34, and Rl-6-22 to Bipolaris sorokiniana. The cultivars and breeding lines showed group resistance to two, three or four pathogens. It was Lines L-8252, L-8134, L-82/60, L-8107, L-8078 (23), L-43-9, L-43-1, L-4, Stb-89, Stb-90, Stb-34, and L-33809-7-3 were observed to carry the recessive allele of the tsn1 gene.</p></sec></trans-abstract><kwd-group xml:lang="ru"><kwd>пшеница</kwd><kwd>генетический анализ</kwd><kwd>ПЦР</kwd><kwd>PtrToxA</kwd><kwd>PtrToxB</kwd><kwd>Tsn1</kwd></kwd-group><kwd-group xml:lang="en"><kwd>wheat</kwd><kwd>seedling resistance</kwd><kwd>adult plant resistance</kwd><kwd>molecular genetics testing</kwd><kwd>PCR</kwd><kwd>PtrToxA</kwd><kwd>PtrToxB</kwd><kwd>Tsn1</kwd></kwd-group><funding-group><funding-statement xml:lang="ru">Работа выполнена при поддержке Российского научного фонда, проект № 19-76-30005. Авторы благодарят рецензентов за их вклад в экспертную оценку этой работы</funding-statement><funding-statement xml:lang="en">The research was financially supported by the Russian Science Foundation, Project No. 19-76-30005. 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