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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">vir-nw</journal-id><journal-title-group><journal-title xml:lang="ru">Труды по прикладной ботанике, генетике и селекции</journal-title><trans-title-group xml:lang="en"><trans-title>Proceedings on applied botany, genetics and breeding</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2227-8834</issn><issn pub-type="epub">2619-0982</issn><publisher><publisher-name>N.I. Vavilov All-Russian Institute of Plant Genetic Resources</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.30901/2227-8834-2023-1-239-248</article-id><article-id custom-type="elpub" pub-id-type="custom">vir-nw-1515</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ОБЗОРЫ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>SURVEYS</subject></subj-group></article-categories><title-group><article-title>Сохранение биоразнообразия растений методами биотехнологии</article-title><trans-title-group xml:lang="en"><trans-title>Conservation of plant biodiversity by biotechnology methods</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-1052-2753</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Ромаданова</surname><given-names>Н. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Romadanova</surname><given-names>N. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>кандидат биологических наук, ассоциированный профессор, ведущий научный сотрудник </p><p>050040 Республика Казахстан, Алматы, ул. Тимирязева, 45 </p></bio><bio xml:lang="en"><p>Cand. Sci. (Biology), Associate Professor, Leading Researcher</p><p>45 Timiryazev St., Almaty 050040, Kazakhstan </p></bio><email xlink:type="simple">nata_romadanova@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-1926-0091</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Кушнаренко</surname><given-names>С. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Kushnarenko</surname><given-names>S. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p> кандидат биологических наук, профессор, заведующая лабораторией </p><p>050040 Республика Казахстан, Алматы, ул. Тимирязева, 45 </p></bio><bio xml:lang="en"><p>Cand. Sci. (Biology), Professor, Head of a Laboratory</p><p>45 Timiryazev St., Almaty 050040, Kazakhstan </p></bio><email xlink:type="simple">svetlana_bio@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>РГП «Институт биологии и биотехнологии растений»</institution><country>Казахстан</country></aff><aff xml:lang="en"><institution>Institute of Plant Biology and Biotechnology</institution><country>Kazakhstan</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2023</year></pub-date><pub-date pub-type="epub"><day>23</day><month>04</month><year>2023</year></pub-date><volume>184</volume><issue>1</issue><fpage>239</fpage><lpage>248</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Ромаданова Н.В., Кушнаренко С.В., 2023</copyright-statement><copyright-year>2023</copyright-year><copyright-holder xml:lang="ru">Ромаданова Н.В., Кушнаренко С.В.</copyright-holder><copyright-holder xml:lang="en">Romadanova N.V., Kushnarenko S.V.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://elpub.vir.nw.ru/jour/article/view/1515">https://elpub.vir.nw.ru/jour/article/view/1515</self-uri><abstract><p>В Республике Казахстан используются несколько способов сохранения генетического материала растений: 1) полевые генные банки в естественных местах произрастания и помологические коллекции; 2) сохранение семян в специализированных хранилищах при температурах: +4°С, –18°С, –196°С; 3) хладохранение in vitro при +4°С, +10 ± 2°С; 4) криоконсервация растительных тканей и органов при температуре –196°С; 5) сохранение ДНК при –80°С.Полевые коллекции растений собраны в ботанических садах, дендрариях, помологических садах, питомниках, крестьянских и фермерских хозяйствах. Наиболее крупная коллекция плодовых и ягодных растений находится в «РГКП Помологический сад» и насчитывает более 40 тыс. образцов.Коллекция семян Республики Казахстан составляет порядка 42 000 образцов. Коллекция зерновых культур сосредоточена в основном в Казахском НИИ земледелия и растениеводства – 1/3 национального генофонда. Основная коллекция семян овощебахчевых культур и картофеля насчитывает 14 700 образцов (Казахский НИИ картофелеводства и овощеводства). Коллекции семян дикорастущих растений имеются в Институте ботаники и фитоинтродукции и в Институте биологии и биотехнологии растений (ИББР), режим хранения +4°С и –18°С. В ИББР дополнительно используется технология криоконсервации в жидком азоте при –196°С.Основная коллекция растений в культуре in vitro хранится в ИББР при +4°С, +10 ± 2°С, и насчитывает более 140 образцов. Криогенная коллекция семян, апикальных меристем, зимующих почек, изолированных зародышевых осей при –196°С находится в только в ИББР, коллекция насчитывает более 700 образцов.</p></abstract><trans-abstract xml:lang="en"><p>Several methods of plant preservation are currently used in the Republic of Kazakhstan: 1) field gene banks in natural growing areas and pomological collections; 2) preservation of seeds at +4°С, –18°С, and –196°С; 3) сold storage of in vitro shoots at +4°С and +10 ± 2°С; 4) cryopreservation of plant tissues and organs at –196°C; 5) preservation of plant DNA at –80°C.Ex situ field collections of plants in Kazakhstan are maintained in botanical gardens of the Republic, arboretums, pomological gardens, nurseries, peasant and farm enterprises, and personal plots. The largest collection of fruit and berry plants is located in the Pomological Garden and includes about 4 thousand varieties, more than 40 thousand hybrids and wild forms of various crops.The Republic of Kazakhstan has about 42,000 accessions in the seed collection. The cereal crop collection is mainly concentrated in the Kazakh Research Institute of Agriculture and Plant Growing – 1/3 of the national genetic resources. The main seed collection of vegetable crops and potatoes (14,700 accessions) is held by the Kazakh Research Institute of Potato and Vegetable Growing. Collections are also available at the Institute of Botany and Phytointroduction and in the Institute of Plant Biology and Biotechnology (IPBB), with the storage mode of + 4°C and –18°C. IPBB also uses cryopreservation technology at –196°C for seed storage.The main in vitro collection of Kazakhstan is kept at the IPBB at + 4°С and +10 ± 2°С; it includes more than 140 accessions of various fruit, berry, nut, vegetable, ornamental, and woody crops, grapes, potatoes, etc. A cryogenic collection of seeds, shoot tips, dormant buds, and embryonic axes in liquid nitrogen (–196°C) is found only in IPBB. The collection includes more than 700 accessions.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>коллекции растений</kwd><kwd>коллекции семян</kwd><kwd>коллекции in vitro</kwd><kwd>криогенные коллекции</kwd></kwd-group><kwd-group xml:lang="en"><kwd>plant collections</kwd><kwd>seed collections</kwd><kwd>in vitro collections</kwd><kwd>cryogenic collections</kwd></kwd-group><funding-group><funding-statement xml:lang="ru">Работа выполнена при поддержке Министерства образования и науки Республики Казахстан в рамках Научно-технической программы BR18554099.</funding-statement><funding-statement xml:lang="en">This research was funded by the Science Committee of the Ministry of Education and Science of the Republic of Kazakhstan (BR18554099).</funding-statement></funding-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Code of the Republic of Kazakhstan dated January 2, 2021: [website]. 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