vir-nwТруды по прикладной ботанике, генетике и селекцииProceedings on applied botany, genetics and breeding2227-88342619-0982N.I. Vavilov All-Russian Institute of Plant Genetic Resources10.30901/2227-8834-2023-1-177-186vir-nw-1509Research ArticleИДЕНТИФИКАЦИЯ ГЕНЕТИЧЕСКОГО РАЗНООБРАЗИЯ КУЛЬТУРНЫХ РАСТЕНИЙ И ИХ ДИКИХ РОДИЧЕЙ ДЛЯ РЕШЕНИЯ ФУНДАМЕНТАЛЬНЫХ И ПРИКЛАДНЫХ ПРОБЛЕМIDENTIFICATION OF THE DIVERSITY OF CULTIVATED PLANTS AND THEIR WILD RELATIVES FOR SOLVING FUNDAMENTAL AND APPLIED PROBLEMSАнализ устойчивости к стеблевой ржавчине и идентификация Sr-генов у интрогрессивных линий яровой мягкой пшеницыAnalysis of resistance to stem rust and identification of Sr genes in introgressive lines of spring bread wheathttps://orcid.org/0000-0001-9439-2102БарановаО. А.BaranovaO. A.

кандидат биологических наук, ведущий научный сотрудник

196608 Россия, Санкт-Петербург, Пушкин, шоссе Подбельского, 3

Cand. Sci. (Biology), Leading Researcher

3 Podbelskogo Hwy., Pushkin, St. Petersburg 196608, Russia 

baranova_oa@mail.ruhttps://orcid.org/0000-0001-8324-9765СибикеевС. Н.SibikeevS. N.

доктор биологических наук, главный научный сотрудник

410010 Россия, Саратов, ул. Тулайкова, 7

Dr. Sci. (Biology), Chief Researcher

7 Tulaikova St., Saratov 410010, Russia 

sibikeev_sergey@mail.ruhttps://orcid.org/0000-0001-8607-2301КоньковаЭ. А.KonkovaE. A.

кандидат сельскохозяйственных наук, ведущий научный сотрудник,

410010 Россия, Саратов, ул. Тулайкова, 7

Cand. Sci. (Agriculture), Leading Researcher

7 Tulaikova St., Saratov 410010, Russia 

baukenowaea@mail.ruВсероссийский научно-исследовательский институт защиты растенийРоссияAll-Russian Institute of Plant ProtectionRussian FederationФедеральный аграрный научный центр Юго-ВостокаРоссияFederal Center of Agriculture Research of the South-East RegionRussian Federation2023220420231841177186Copyright © Баранова О.А., Сибикеев С.Н., Конькова Э.А., 20232023Баранова О.А., Сибикеев С.Н., Конькова Э.А.Baranova O.A., Sibikeev S.N., Konkova E.A.This work is licensed under a Creative Commons Attribution 4.0 License.https://elpub.vir.nw.ru/jour/article/view/1509

Актуальность. В связи с увеличением вредоносности на территории Поволжья стеблевой ржавчины пшеницы (возбудитель – Puccinia graminis f. sp. tritici Erikss. & E. Henn.) и вероятностью заноса агрессивной расы Ug99 принципиальное значение приобретает оценка генетического разнообразия селекционного материала пшеницы и идентификация эффективных Sr-генов.Материалы и методы. В работе анализировали 90 интрогрессивных линий яровой мягкой пшеницы селекции Федерального аграрного научного центра Юго-Востока, устойчивых к P. graminis f. sp. tritici. Для выявления генов устойчивости Sr24/Lr24, Sr25/Lr19, Sr26, Sr28, Sr31/Lr26, Sr32, Sr36, Sr38/Lr37, Sr39 и Sr57/Lr34 использовали молекулярные маркеры. Устойчивость растений анализировали по стандартным методикам, тип реакции определяли по шкале Стекмана.Результаты. У селекционных линий яровой мягкой пшеницы идентифицированы гены Sr31/Lr26, Sr25/Lr19, Sr57/Lr34, Sr38/Lr37 и Sr39/Lr35. Ген Sr25 выявлен у 51 линии (56,7% изученных образцов), Sr31 – у 41 линии (45,6%), Sr57/Lr34 – у 5 линий, Sr38 – у 10 линий и Sr39 – у одной линии. Идентифицированы комбинации генов устойчивости: Sr31+Sr25 – у 28 линий (31,1%), Sr25+Sr38 – у 5 линий и Sr25+Sr39 – у одной линии. Гены Sr24/Lr24, Sr26, Sr28, Sr32 и Sr36 не выявлены.Заключение. Выделены высокоустойчивые к P. graminis f. sp. tritici линии яровой мягкой пшеницы с перспективными комбинациями генов Sr31+Sr25, Sr25+Sr38 и Sr25+Sr39, которые могут быть использованы в российских селекционных программах.

Background. Due to the increase in the harmfulness of wheat stem rust (Puccinia graminis f. sp. tritici Erikss. & E. Henn.) in the Volga region, and the likelihood of the spread of the aggressive Ug99 race, an assessment of the genetic diversity of wheat breeding lines and identification of effective Sr genes are of fundamental importance.Materials and methods. Ninety spring bread wheat introgressive lines with stem rust resistance, developed at the Federal Center of Agriculture Research of the South-East Region, were analyzed. Molecular markers were used to identify resistance genes: Sr24/Lr24, Sr25/Lr19, Sr26, Sr28, Sr31/Lr26, Sr32, Sr36, Sr38/Lr37, Sr39 and Sr57/Lr34. The analysis of plants for resistance was carried out according to standard methods; the Stakman scale was applied to determine the type of reaction.Results. The genes Sr31/Lr26, Sr25/Lr19, Sr57/Lr34, Sr38/Lr37 and Sr39/Lr35 were identified in the analyzed breeding lines. Sr25 was found in 51 lines (56,7% of samples), Sr31 in 41 lines (45.6%), Sr57/Lr34 in 5 lines, Sr38 in 10 lines and Sr39 in one line. Combinations of resistance genes were identified: Sr31+Sr25 in 28 lines (31.1%), Sr25+Sr38 in 5 lines, and Sr25+Sr39 in one line. The Sr24/Lr24, Sr26, Sr28, Sr32 and Sr36 genes were not identified.Conclusion. As a result, promising highly resistant introgressive wheat lines with promising combinations of Sr31+Sr25, Sr25+Sr38 and Sr25+Sr39 genes were identified. They can be used in Russian breeding programs for immunity.

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The authors declare that there are no conflicts of interest present.