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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">vir-nw</journal-id><journal-title-group><journal-title xml:lang="ru">Труды по прикладной ботанике, генетике и селекции</journal-title><trans-title-group xml:lang="en"><trans-title>Proceedings on applied botany, genetics and breeding</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2227-8834</issn><issn pub-type="epub">2619-0982</issn><publisher><publisher-name>N.I. Vavilov All-Russian Institute of Plant Genetic Resources</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.30901/2227-8834-2022-3-123-131</article-id><article-id custom-type="elpub" pub-id-type="custom">vir-nw-1352</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ГЕНЕТИКА КУЛЬТУРНЫХ РАСТЕНИЙ И ИХ ДИКИХ РОДИЧЕЙ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>GENETICS OF CULTIVATED PLANTS AND THEIR WILD RELATIVES</subject></subj-group></article-categories><title-group><article-title>Аллельное состояние проламин-кодирующих локусов нового сорта овса посевного ‘Тоболяк’</article-title><trans-title-group xml:lang="en"><trans-title>Allelic state of prolamin-coding loci in the new oat cultivar ‘Tobolyak’</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-1570-9595</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Любимова</surname><given-names>А. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Lyubimova</surname><given-names>A. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Любимова Анна Валерьевна - кандидат биологических наук, заведующая лабораторией.</p><p>625026, Тюмень, ул. Малыгина, 86.</p></bio><bio xml:lang="en"><p>Anna V. Lyubimova - Cand. Sci. (Biology), Head of a Laboratory, Tyumen Scientific Center of the Siberian Branch of the Russian Academy  of  Sciences.</p><p>86 Malygina St.,  Tyumen  625026.</p></bio><email xlink:type="simple">ostapenkoav88@yandex.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-2923-9448</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Фомина</surname><given-names>М. Н.</given-names></name><name name-style="western" xml:lang="en"><surname>Fomina</surname><given-names>M. N.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Фомина Мария Николаевна - кандидат сельскохозяйственных наук, ведущий научный сотрудник.</p><p>625501, Тюменская обл., Тюменский р-н, п. Московский, ул. Бурлаки, 2.</p></bio><bio xml:lang="en"><p>Maria N. Fomina - Cand. Sci. (Agriculture), Leading Researcher, Tyumen Scientific Center of the Siberian Branch of the Russian Academy of Sciences.</p><p>86 Malygina St., Tyumen 625026.</p></bio><email xlink:type="simple">maria_f72@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-3672-6060</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Еремин</surname><given-names>Д. И.</given-names></name><name name-style="western" xml:lang="en"><surname>Eremin</surname><given-names>D. I.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Еремин Дмитрий Иванович - доктор биологических наук, ведущий научный сотрудник.</p><p>625026, Тюмень, ул. Малыгина, 86.</p></bio><bio xml:lang="en"><p>Dmitry I. Eremin - Dr. Sci. (Biology), Leading Researcher, Tyumen Scientific Center of the Siberian Branch of the Russian Academy of Sciences.</p><p>86 Malygina St., Tyumen 625026.</p></bio><email xlink:type="simple">soil-tyumen@yandex.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Мамаева</surname><given-names>В. С.</given-names></name><name name-style="western" xml:lang="en"><surname>Mamaeva</surname><given-names>V. S.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Мамаева Виктория Сергеевна - стажер-исследователь.</p><p>625026, Тюмень, ул. Малыгина, 86.</p></bio><bio xml:lang="en"><p>Victoria S. Mamaeva - Intern Researcher, Tyumen Scientific Center of the Siberian Branch of the Russian Academy of Sciences.</p><p>86 Malygina St., Tyumen 625026.</p></bio><email xlink:type="simple">mamaeva.vs.b23@ati.gausz.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Мишечкина</surname><given-names>В. С.</given-names></name><name name-style="western" xml:lang="en"><surname>Mishechkina</surname><given-names>V. S.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Мишечкина Валерия Сергеевна - младший научный сотрудник.</p><p>625026, Тюмень, ул. Малыгина, 86.</p></bio><bio xml:lang="en"><p>Valeria S. Mishechkina - Associate Researcher, Tyumen Scientific Center of the Siberian Branch of the Russian Academy of Sciences.</p><p>86 Malygina St., Tyumen 625026.</p></bio><email xlink:type="simple">leokin404@gmail.com</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-3038-7352</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Брагин</surname><given-names>Н. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Bragin</surname><given-names>N. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Брагин Николай Александрович - младший научный сотрудник.</p><p>625026, Тюмень, ул. Малыгина, 86.</p></bio><bio xml:lang="en"><p>Nikolay A. Bragin - Associate Researcher, Tyumen Scientific Center of the Siberian Branch of the Russian Academy of Sciences.</p><p>86 Malygina St., Tyumen 625026.</p></bio><email xlink:type="simple">gen.selekciya72@gmail.com</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Белоусов</surname><given-names>С. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Belousov</surname><given-names>S. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Белоусов Сергей Александрович - младший научный сотрудник.</p><p>625026, Тюмень, ул. Малыгина, 86.</p></bio><bio xml:lang="en"><p>Sergei A. Belousov - Associate Researcher, Tyumen Scientific Center of the Siberian Branch of the Russian Academy of Sciences.</p><p>86 Malygina St., Tyumen 625026.</p></bio><email xlink:type="simple">gen.selekciya72@gmail.com</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Брагина</surname><given-names>М. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Bragina</surname><given-names>M. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Брагина Мария Владимировна - младший научный сотрудник.</p><p>625026, Тюмень, ул. Малыгина, 86.</p></bio><bio xml:lang="en"><p>Maria V. Bragina - Associate Researcher, Tyumen Scientific Center of the Siberian Branch of the Russian Academy of Sciences.</p><p>86 Malygina St., Tyumen 625026.</p></bio><email xlink:type="simple">gen.selekciya72@gmail.com</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>Федеральный исследовательский центр Тюменский научный центр Сибирского отделения Российской академии наук</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Tyumen Scientific Centre, Siberian Branch of the Russian Academy of Sciences</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2022</year></pub-date><pub-date pub-type="epub"><day>04</day><month>10</month><year>2022</year></pub-date><volume>183</volume><issue>3</issue><fpage>123</fpage><lpage>131</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Любимова А.В., Фомина М.Н., Еремин Д.И., Мамаева В.С., Мишечкина В.С., Брагин Н.А., Белоусов С.А., Брагина М.В., 2022</copyright-statement><copyright-year>2022</copyright-year><copyright-holder xml:lang="ru">Любимова А.В., Фомина М.Н., Еремин Д.И., Мамаева В.С., Мишечкина В.С., Брагин Н.А., Белоусов С.А., Брагина М.В.</copyright-holder><copyright-holder xml:lang="en">Lyubimova A.V., Fomina M.N., Eremin D.I., Mamaeva V.S., Mishechkina V.S., Bragin N.A., Belousov S.A., Bragina M.V.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://elpub.vir.nw.ru/jour/article/view/1352">https://elpub.vir.nw.ru/jour/article/view/1352</self-uri><abstract><sec><title>Актуальность</title><p>Актуальность. Для того чтобы наиболее полно раскрыть преимущества сорта и эффективно использовать его в производстве, необходимо обеспечить высокие посевные и сортовые качества семян, что требует улучшения селекционно-семеноводческой работы. Все шире в первичном семеноводстве применяются биотехнологические методы. Высокую эффективность показал метод электрофореза запасных белков семян – проламинов. Целью исследований была оценка биотипного состава и аллельного состояния проламин-кодирующих локусов нового сорта ярового овса ‘Тоболяк’ для дальнейшего использования в первичном семеноводстве.</p></sec><sec><title>Материалы и методы</title><p>Материалы и методы. Методом нативного электрофореза было исследовано 1519 семей сорта ‘Тоболяк’. Для анализов методом случайной выборки отбирали по три зерновки от каждой семьи. Электрофорез проводили в вертикальных пластинах 13,2-процентного полиакриламидного геля при постоянном напряжении 500 V в течение 4,5–5,0 часов.</p></sec><sec><title>Результаты</title><p>Результаты. В результате исследования семей сорта ‘Тоболяк’ выявлено 13 типов спектра авенина с частотой встречаемости от 96,36 до 0,07%. Описан новый блок компонентов авенина, контролируемый аллелем локуса Avn B. Данному блоку присвоен номер 8. Установлено, что сорт ‘Тоболяк’ создан путем скрещивания минорного биотипа сорта ‘Таежник’ (Avn 4.4.2) и первого биотипа сорта ‘Орион’ (Avn 2.8.2). Основу сорта ‘Тоболяк’ составляет один генотип с формулой авенина Avn 4.8.2. Остальные типы спектров возникли в результате скрещивания различных биотипов сортов ‘Таёжник’ и ‘Орион’, а также механического засорения. Для поддержания генетической стабильности сорта ‘Тоболяк’ рекомендовано использовать в процессе оригинального семеноводства только семьи с первым типом спектра.</p></sec></abstract><trans-abstract xml:lang="en"><sec><title>Background</title><p>Background. In order to fully reveal the advantages of a cultivar and use it effectively in production, it is necessary to ensure high sowing and varietal qualities of seeds, which requires improvement in breeding and seed production. Biotechnological methods are increasingly used in initial seed production. High efficiency was shown by the method of electrophoresis of seed storage proteins – prolamins. The aim of this study was to assess the biotype composition and allelic state of the prolamin-coding loci in the new spring oat cultivar ‘Tobolyak’ for its further use in initial seed production.</p></sec><sec><title>Materials and methods</title><p>Materials and methods. In 2021, 1519 families of cv. ‘Tobolyak’ were studied using the method of native electrophoresis. Three grains from each family were selected for analyses by random sampling. Electrophoresis was carried out in vertical plates of 13.2% polyacrylamide gel at a constant voltage of 500 V for 4.5–5.0 hours.</p></sec><sec><title>Results and conclusion</title><p>Results and conclusion. The study of cv. ‘Tobolyak’ families resulted in identifying 13 types of avenin patterns with a frequency of occurrence from 96.36 to 0.07%. A new block of avenin components controlled by the allele of the Avn B locus was described. Number 8 was assigned to this block. It was established that cv. ‘Tobolyak’ had been developed by crossing a minor biotype of cv. ‘Taezhnik’ (Avn 4.4.2) with the first biotype of cv. ‘Orion’ (Avn 2.8.2). The basis of cv. ‘Tobolyak’ was one genotype with the Avn 4.8.2 avenin formula. The remaining biotypes arose as a result of crossing the biotypes of cvs. ‘Taezhnik’ and ‘Orion’ as well as through mechanical contamination. To maintain the genetic stability of cv. ‘Tobolyak’, it is recommended to use only families of the first biotype in the process of original seed production.</p></sec></trans-abstract><kwd-group xml:lang="ru"><kwd>первичное семеноводство</kwd><kwd>электрофорез</kwd><kwd>авенин-кодирующие локусы</kwd><kwd>блоки компонентов авенина</kwd><kwd>сортовая чистота</kwd><kwd>биотипный состав сорта</kwd></kwd-group><kwd-group xml:lang="en"><kwd>initial seed production</kwd><kwd>electrophoresis</kwd><kwd>avenin-coding loci</kwd><kwd>blocks of avenin components</kwd><kwd>varietal purity</kwd><kwd>biotype composition of the cultivar</kwd></kwd-group><funding-group><funding-statement xml:lang="ru">Работа выполнена по госзаданию № 122011300103-0 и при поддержке Западно-Сибирского межрегионального научно-образовательного центра мирового уровня. Авторы благодарят рецензентов за их вклад в экспертную оценку этой работы.</funding-statement><funding-statement xml:lang="en">The work was carried out within the framework of the state task (No. 122011300103-0) and supported by the West Siberian Interregional Scientific and Educational Center. The authors thank the reviewers for their contribution to the peer review of this work.</funding-statement></funding-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Campbell M.T., Hu H., Yeats T.H., Caffe-Treml M., Gutierrez L., Smith K.P. et al. Translating insights from the seed metabolome into improved prediction for lipid-composition traits in oat (Avena sativa L.). Genetics. 2021;217(3):iyaa043. DOI: 10.1093/genetics/iyaa043</mixed-citation><mixed-citation xml:lang="en">Campbell M.T., Hu H., Yeats T.H., Caffe-Treml M., Gutierrez L., Smith K.P. et al. 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