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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">vir-nw</journal-id><journal-title-group><journal-title xml:lang="ru">Труды по прикладной ботанике, генетике и селекции</journal-title><trans-title-group xml:lang="en"><trans-title>Proceedings on applied botany, genetics and breeding</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2227-8834</issn><issn pub-type="epub">2619-0982</issn><publisher><publisher-name>N.I. Vavilov All-Russian Institute of Plant Genetic Resources</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.30901/2227-8834-2022-3-76-84</article-id><article-id custom-type="elpub" pub-id-type="custom">vir-nw-1348</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>КОЛЛЕКЦИИ МИРОВЫХ ГЕНЕТИЧЕСКИХ РЕСУРСОВ КУЛЬТУРНЫХ РАСТЕНИЙ ДЛЯ РАЗВИТИЯ ПРИОРИТЕТНЫХ НАПРАВЛЕНИЙ СЕЛЕКЦИИ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>COLLECTIONS OF THE WORLD’S CROP GENETIC RESOURCES FOR THE DEVELOPMENT OF PRIORITY PLANT BREEDING TRENDS</subject></subj-group></article-categories><title-group><article-title>Изучение разнообразия окраски зерновки ячменя в коллекции ВИР</article-title><trans-title-group xml:lang="en"><trans-title>Studying grain color diversity in the barley collection of VIR</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-1692-7578</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Глаголева</surname><given-names>А. Ю.</given-names></name><name name-style="western" xml:lang="en"><surname>Glagoleva</surname><given-names>A. Y.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Глаголева Анастасия Юрьевна - младший научный сотрудник, ФИЦ ИЦиГ СО РАН; ФИЦ ВИР им. Н.И. Вавилова.</p><p>630090, Новосибирск, пр. Академика Лаврентьева, 10; 190000, Санкт-Петербург, ул. Б. Морская, 42, 44.</p></bio><bio xml:lang="en"><p>Anastasia Y. Glagoleva - Associate Researcher, Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences; N.I. Vavilov All-Russian Institute of Plant Genetic Resources.</p><p>10 Akademika Lavrentyeva Ave., Novosibirsk 630090; 42, 44 Bolshaya Morskaya Street, St. Petersburg 190000.</p></bio><email xlink:type="simple">glagoleva@bionet.nsc.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Новокрещёнов</surname><given-names>Л. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Novokreschenov</surname><given-names>L. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Новокрещёнов Леонид  Александрович – лаборант.</p><p>630090, Новосибирск, пр. Академика Лаврентьева, 10.</p></bio><bio xml:lang="en"><p>Leonid A. Novokreschenov - Laboratory Assistant, Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences.</p><p>10 Akademika Lavrentyeva Ave., Novosibirsk 630090.</p></bio><email xlink:type="simple">NovokreshchyonovLA@bionet.nsc.ru</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-5289-8631</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Шоева</surname><given-names>О. Ю.</given-names></name><name name-style="western" xml:lang="en"><surname>Shoeva</surname><given-names>O. Y.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Шоева Олеся Юрьевна - кандидат биологических наук, старший научный сотрудник, ФИЦ ИЦиГ СО РАН; ФИЦ ВИР им. Н.И. Вавилова .</p><p>630090, Новосибирск, пр. Академика Лаврентьева, 10; 190000, Санкт-Петербург, ул. Б. Морская, 42, 44.</p></bio><bio xml:lang="en"><p>Olesya Y. Shoeva - Cand. Sci. (Biology), Senior Researcher, Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences; N.I. Vavilov All-Russian Institute of Plant Genetic  Resources.</p><p>10 Akademika Lavrentyeva Ave., Novosibirsk 630090; 42, 44 Bolshaya  Morskaya  Street, St. Petersburg  190000.</p></bio><email xlink:type="simple">olesya_ter@bionet.nsc.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-3990-6526</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Ковалева</surname><given-names>О. Н.</given-names></name><name name-style="western" xml:lang="en"><surname>Kovaleva</surname><given-names>O. N.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Ковалева Ольга Николаевна - кандидат биологических наук, ведущий научный сотрудник.</p><p>190000, Санкт-Петербург, ул. Б. Морская, 42, 44.</p></bio><bio xml:lang="en"><p>Olga N. Kovaleva - Cand.  Sci.  (Biology),  Leading  Researcher,  N.I. Vavilov  All-Russian  Institute  of  Plant  Genetic  Resources.</p><p>42, 44 Bolshaya Morskaya Street, St. Petersburg 190000.</p></bio><email xlink:type="simple">o.kovaleva@vir.nw.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-8470-8254</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Хлесткина</surname><given-names>Е. К.</given-names></name><name name-style="western" xml:lang="en"><surname>Khlestkina</surname><given-names>E. K.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Хлесткина Елена Константиновна - доктор биологических наук, профессор РАН, директор, ФИЦ ВИР им. Н.И. Вавилова; ФИЦ ИЦиГ СО РАН.</p><p>190000, Санкт-Петербург, ул. Б. Морская, 42, 44; 630090, Новосибирск, пр. Академика Лаврентьева, 10.</p></bio><bio xml:lang="en"><p>Elena K. Khlestkina - Dr. Sci. (Biology), Professor of the RAS, Director, N.I. Vavilov All-Russian Institute of Plant Genetic Resources; Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences.</p><p>42, 44 Bolshaya Morskaya Street, St. Petersburg 190000; 10 Akademika Lavrentyeva Ave., Novosibirsk 630090.</p></bio><email xlink:type="simple">director@vir.nw.ru</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru">Федеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук; Федеральный исследовательский центр Всероссийский институт генетических ресурсов растений имени Н.И. Вавилова<country>Россия</country></aff><aff xml:lang="en">Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences; N.I. Vavilov All­Russian Institute of Plant Genetic Resources<country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru">Федеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук<country>Россия</country></aff><aff xml:lang="en">Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences<country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2022</year></pub-date><pub-date pub-type="epub"><day>03</day><month>10</month><year>2022</year></pub-date><volume>183</volume><issue>3</issue><fpage>76</fpage><lpage>84</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Глаголева А.Ю., Новокрещёнов Л.А., Шоева О.Ю., Ковалева О.Н., Хлесткина Е.К., 2022</copyright-statement><copyright-year>2022</copyright-year><copyright-holder xml:lang="ru">Глаголева А.Ю., Новокрещёнов Л.А., Шоева О.Ю., Ковалева О.Н., Хлесткина Е.К.</copyright-holder><copyright-holder xml:lang="en">Glagoleva A.Y., Novokreschenov L.A., Shoeva O.Y., Kovaleva O.N., Khlestkina E.K.</copyright-holder><license license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://elpub.vir.nw.ru/jour/article/view/1348">https://elpub.vir.nw.ru/jour/article/view/1348</self-uri><abstract><sec><title>Актуальность</title><p>Актуальность.  Темная  окраска зерновки ячменя (Hordeum vulgare L.)  обусловлена  синтезом  и накоплением  двух групп пигментов полифенольной природы – антоцианами и меланинами. Целью данного исследования стало изучение разнообразия ячменя из коллекции ВИР по признаку окраски зерновки.</p></sec><sec><title>Материалы и методы</title><p>Материалы и методы. Для анализа пигментного состава зерна были выбраны 150 образцов ячменя с окрашенной зерновкой из коллекции ВИР. Идентификация наличия антоцианов и меланинов в оболочках зерна проводилась при помощи качественных реакции.</p></sec><sec><title>Результаты и обсуждение</title><p>Результаты и обсуждение. Показано, что для большинства исследуемых образцов темная окраска зерна обусловлена самостоятельным накоплением меланина. Для 11,3% образцов не выявили присутствия антоцианов и меланинов в оболочках зерновки, и, вероятно, их окраска связана с повышенным содержанием других пигментов полифенольной природы – проантоцианидинов. Образцы с меламиновой окраской зерна были представлены во всех выделенных географических группах, в то же время другие типы пигментации наиболее равномерно представлены в регионах, для которых характерно широкое генетическое разнообразие ячменя – Африка, Восточная Азия и Ближний Восток.</p></sec><sec><title>Заключение</title><p>Заключение. В результате анализа образцов ячменя из коллекции ВИР по признаку окраски зерновки показано, что темная окраска зерна преимущественно связана с накоплением меланина, причем данный тип пигментации преобладает во всех выделенных географических регионах. Полученные результаты позволяют полнее охарактеризовать коллекцию ячменя и расширить возможности ее использования.</p></sec></abstract><trans-abstract xml:lang="en"><sec><title>Background</title><p>Background. Dark color of barley grain (Hordeum vulgare L.) can be caused by the synthesis and accumulation of two types of polyphenolic pigments – anthocyanins and melanins, which perform important functions in plant life, participating in the regulation of growth and development, and protecting plants from adverse environmental conditions. The aim of this study was to investigate the diversity of barley in the VIR collection in the context of grain color.</p></sec><sec><title>Materials and methods</title><p>Materials and methods. To analyze the pigment composition of the grain, 150 barley accessions with colored grains were selected from the VIR collection. Anthocyanins and melanins in grain husk were identified using qualitative reactions.</p></sec><sec><title>Results and discussion</title><p>Results and discussion. It was shown that in 60% of the accessions the dark color of their grain was induced by independent accumulation of melanin, while the accessions characterized by accumulation of only anthocyanins, and those with combined accumulation of anthocyanins and melanins, were 14.6% and 14%, respectively. For 11.3% of the accessions the presence of anthocyanins and melanins in grain husk was not found; their pigmentation could presumably be associated with an increased content of other polyphenolic pigments – proanthocyanidins. Accessions with melanin in grain predominated in all identified geographic groups, while other types of pigmentation were most evenly represented in the regions with the widest genetic diversity of barleys – Africa, East Asia, and the Middle East.</p></sec><sec><title>Conclusion</title><p>Conclusion. Dark pigmentation of barley grain was shown to be mainly associated with the accumulation of melanin, and this type of pigmentation prevails in all geographical regions identified. The results obtained made it possible to describe the barley collection more fully and expand the possibilities of its utilization.</p></sec></trans-abstract><kwd-group xml:lang="ru"><kwd>Hordeum vulgare L.</kwd><kwd>меланин</kwd><kwd>антоцианы</kwd><kwd>генетические ресурсы растении</kwd></kwd-group><kwd-group xml:lang="en"><kwd>Hordeum vulgare L.</kwd><kwd>melanins</kwd><kwd>anthocyanins</kwd><kwd>plant genetic resources</kwd></kwd-group><funding-group xml:lang="ru"><funding-statement>Исследование выполнено при поддержке гранта РФФИ № 20-316-90033, а также бюджетного проекта ИЦиГ СО РАН FWNR-2022-0007. Авторы благодарят рецензентов за их вклад в экспертную оценку этой работы.</funding-statement></funding-group><funding-group xml:lang="en"><funding-statement>The study was supported by the Russian Foundation for Basic Research, Grant No. 20-316-90033, and ICG Budget Project FWNR-2022-0007. The authors thank the reviewers for their contribution to the peer review of this work.</funding-statement></funding-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Aastrup S., Outtrup H., Erdal K. Location of the proanthocyanidins in the barley grain. Carlsberg Research Communications. 1984;49:105-109. DOI: 10.1007/BF02913969</mixed-citation><mixed-citation xml:lang="en">Aastrup S., Outtrup H., Erdal K. Location of the proanthocyanidins in the barley grain. Carlsberg Research Communications. 1984;49:105-109. 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