vir-nwТруды по прикладной ботанике, генетике и селекцииProceedings on applied botany, genetics and breeding2227-88342619-0982N.I. Vavilov All-Russian Institute of Plant Genetic Resources10.30901/2227-8834-2022-3-59-65vir-nw-1346Research ArticleИЗУЧЕНИЕ И ИСПОЛЬЗОВАНИЕ ГЕНЕТИЧЕСКИХ РЕСУРСОВ РАСТЕНИЙSTUDYING AND UTILIZATION OF PLANT GENETIC RESOURCESХимический состав и антибактериальная активность эфирных масел Amomum longiligulare из ВьетнамаChemical constituents and antibacterial activity of essential oils in Amomum longiligulare from Vietnamhttps://orcid.org/0000-0003-0875-5259ХунгН.HungN.

Хунг Нгуен - преподаватель, Вьетнамско-венгерский промышленный университет.

12700, Ханой, Сон Таи, ул. Хуу Нги, 16.

Nguyen Hung - Lecturer, Vietnam–Hungary Industrial University.

16 Huu Nghi, Son Tay, Hanoi 12700.

n.hung.vhiu@gmail.comhttps://orcid.org/0000-0002-3342-370XТоанН. Х.ToanN. H.

Тоан Нгуен Хуу - научный сортудник, Университет Куангнам.

51100, пров. Куангнам, Там Ки, ул. Хунг Вуонг, 102.

Nguyen Huu Toan - Researcher, Quang Nam University.

102 Hung Vuong, Tam Ky, Quangnam 51100.

toanqn59@gmail.comВьетнамско-венгерский промышленный университетВьетнамVietnam–Hungary Industrial UniversityViet NamУниверситет КуангнамВьетнамQuang Nam UniversityViet Nam20220310202218335965Copyright © Хунг Н., Тоан Н.Х., 20222022Хунг Н., Тоан Н.Х.Hung N., Toan N.H.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://elpub.vir.nw.ru/jour/article/view/1346

В этой статье сообщается о химическом составе и антибактериальной активности эфирных масел из листьев, корневищ и плодов Amomum longiligulare T.L. Wu (Zingiberaceae), полученных путем гидродистилляции с помощью микроволновой печи. Эфирные масла анализировали методами газовой хроматографии – масс-спектрометрии. Значения минимальной ингибирующей концентрации (МИК) измеряли с помощью анализа микроразведений в бульонной среде. Выход масла из листьев, корневищ и плодов A. longiligulare составлял 0,23%, 0,27% и 1,93% (об./вес.) соответственно в расчете на сухую массу. Эфирное масло листьев состоит в основном из α-гумулена (28,4%), α-пинена (24,9%), β-кариофиллена (17,3%), эпоксида гумулена II (7,3%) и β-пинена (4,7%). Основными соединениями эфирного масла корневища были β-кариофиллен (28,7%), бициклогермакрен (17,1%), эпоксид гумулена II (10,5%), камфен (7,9%) и α-пинен (5,7%). Камфора (40,7%) и борнилацетат (34,2%) были основными составляющими масла плодов. Эфирные масла продемонстрировали антимикробную активность в отношении Staphylococcus aureus, Bacillus cereus, Escherichia coli и Pseudomonas aeruginosa со значениями МИК от 200 до 400 мкг/мл. Таким образом, эфирные масла A. longiligulare являются источником многообещающих антибактериальных средств. Это первое сообщение о химическом составе и антибактериальнои активности эфирного масла A. longiligulare, полученного путем гидродистилляции с помощью микроволновой печи.

This paper reports the chemical constituents and the antibacterial activity of essential oils from the leaves, rhizomes, and fruits of Amomum longiligulare T.L. Wu (Zingiberaceae) obtained by microwave-assisted hydrodistillation. The essential oils were analyzed by gas chromatography–mass spectrometry techniques. The minimum inhibitory concentration (MIC) values were measured by the broth microdilution assay. The oil yields of leaves, rhizomes and fruits from A. longiligulare were 0.23%, 0.27% and 1.93% (v/w), respectively, calculated on a dry weight basis. The leaf essential oil comprised mainly α-humulene (28.4%), α-pinene (24.9%), β-caryophyllene (17.3%), humulene epoxide II (7.3%), and β-pinene (4.7%). The major compounds of the rhizome essential oil were β-caryophyllene (28.7%), bicyclogermacrene (17.1%), humulene epoxide II (10.5%), camphene (7.9%), and α-pinene (5.7%). Camphor (40.7%) and bornyl acetate (34.2%) were the main constituents of the fruit oil. The essential oils demonstrated antimicrobial activities against Staphylococcus aureus, Bacillus cereus, Escherichia coli, and Pseudomonas aeruginosa with the MIC values ranging from 200 to 400 μg/mL. In summary, the A. longiligulare essential oils are a source of promising antibacterial agents. This is the first report on the chemical composition and antibacterial activity of A. longiligulare essential oil obtained by microwave-assisted hydrodistillation.

Zingiberaceaeэфирное маслоантибактериальная активностьгидродистилляция с помощью микроволновой печиZingiberaceaeessential oilantibacterial activitymicrowave-assisted hydrodistillationАвторы выражают признательность г-ну Тран Ван Киен за его помощь в полевых исследованиях и сборе образцов. Авторы благодарят рецензентов за их вклад в экспертную оценку этой работы.The authors would like to thank Mr. Tran Van Kien for his assistance with the field studies and sample collection. The authors thank the reviewers for their contribution to the peer review of this work.ReferencesAdams R.P. Identification of essential oil components by gas chromatography/quadrupole mass spectrometry. 4th ed. Carol Stream, IL: Allured Publishing Corporation; 2007.Adams R.P. Identification of essential oil components by gas chromatography/quadrupole mass spectrometry. 4th ed. Carol Stream, IL: Allured Publishing Corporation; 2007.Anh T.T., Bao Ngoc N.H., Phuc N.D., Nhat D.D., Danh P.H., Bach L.G. 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The authors declare that there are no conflicts of interest present.