vir-nwТруды по прикладной ботанике, генетике и селекцииProceedings on applied botany, genetics and breeding2227-88342619-0982N.I. Vavilov All-Russian Institute of Plant Genetic Resources10.30901/2227-8834-2022-1-224-235vir-nw-1207Research ArticleОБЗОРЫSURVEYSДНК-маркеры в селекции овса на устойчивость к корончатой ржавчине (обзор)DNA markers in oat breeding for crown rust resistance (a review)https://orcid.org/0000-0002-5171-2476БакулинаА. В.BakulinaA. V.

Анна Владимировна Бакулина, кандидат биологических наук, старший научный сотрудник, зав. лабораторией

610007, Киров, ул. Ленина, 166а

Anna V. Bakulina, Cand. Sci. (Biology), Senior Researcher, Head of a Laboratory

166a Lenina St., Kirov 610007

mol-biol@fanc-sv.ruhttps://orcid.org/0000-0002-0638-4258НовоселоваН. В.NovoselovaN. V.

Нина Владиславовна Новоселова, младший научный сотрудник

610007, Киров, ул. Ленина, 166а

Nina V. Novoselova, Associate Researcher

166a Lenina St., Kirov 610007

syllvana@mail.ruhttps://orcid.org/0000-0002-7878-4891СавинцеваЛ. С.SavintsevaL. S.

Лариса Сергеевна Савинцева, кандидат биологических наук, научный сотрудник

610007, Киров, ул. Ленина, 166а

Larisa S. Savintseva, Cand. Sci. (Biology), Researcher

166a Lenina St., Kirov 610007

savinceva.l@mail.ruhttps://orcid.org/0000-0002-3491-499XБаталоваГ. А.BatalovaG. A.

Галина Аркадьевна Баталова, академик РАН, зам. директора по селекционной работе

610007, Киров, ул. Ленина, 166а

Galina A. Batalova, Full Member (Academician) of the RAS, Deputy Director for Breeding Work

166a Lenina St., Kirov 610007

g.batalova@mail.ruФедеральный аграрный научный центр Северо-Востока имени Н.В. РудницкогоРоссияFederal Agricultural Research Center of the North-East named N.V. RudnitskyRussian Federation2022190420221831224235Copyright © Бакулина А.В., Новоселова Н.В., Савинцева Л.С., Баталова Г.А., 20222022Бакулина А.В., Новоселова Н.В., Савинцева Л.С., Баталова Г.А.Bakulina A.V., Novoselova N.V., Savintseva L.S., Batalova G.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://elpub.vir.nw.ru/jour/article/view/1207

Цель настоящего обзора – анализ и обобщение имеющейся информации о ДНК-маркерах, разработанных для селекции овса (Avena sativa L.) на устойчивость к корончатой ржавчине. В статье рассматриваются механизмы устойчивости овса к возбудителю корончатой ржавчины – Puccinia coronata Corda f. sp. avenae Erikss. Особое внимание уделено расоспецифической устойчивости, обусловленной действием Pc-генов, и неспецифической устойчивости, контролируемой преимущественно локусами количественных признаков. Обсуждаются стратегии создания устойчивых генотипов и роль молекулярных маркеров в селекции овса на устойчивость к патогену. На данном этапе исследования сосредоточены в основном на поиске и разработке молекулярных маркеров, сцепленных с генами расоспецифической устойчивости овса к P. coronata.В статье отражены преимущества и недостатки существующих ДНК-маркеров. В качестве наиболее доступных для внедрения в практическую селекцию овса нами рекомендованы прошедшие валидацию SCAR- и STS-маркеры к генам Pc39, Pc68, Pc91, Pc94. Приводятся результаты исследований по определению локусов неспецифической устойчивости к P. coronata. В целом применение ДНК-маркеров имеет значительный потенциал для создания устойчивых к корончатой ржавчине генотипов овса в современных условиях. ДНК-маркеры различных типов рекомендованы для практического использования, в частности для пирамидирования генов и увеличения срока устойчивости новых сортов. Внедрение ДНК-маркеров в селекцию овса будет возрастать с накоплением молекулярно-генетических данных и совершенствованием технологий выявления генов и локусов, связанных как с расоспецифической, так и неспецифической устойчивостью овса к P. coronata.

Crown rust is the most harmful disease of oat (Avena sativa L.) around the world. The purpose of this review is to analyze and generalize the available information about DNA markers developed for oat breeding for resistance to crown rust. The review reveals the mechanisms of the A. sativa resistance to the fungus Puccinia coronata Corda f. sp. avenae Erikss. which causes crown rust disease. Special attention is paid to the race-specific resistance caused by the action of Pc genes and the nonspecific resistance controlled mainly by the loci of quantitative traits. Strategies for creating resistant genotypes and the role of molecular markers in oat breeding for crown rust resistance are discussed. Currently, research is focused mainly on the search for and development of molecular markers related to the oat race-specific resistance to P. coronata.The article presents the technological advantages and disadvantages of the existing DNA markers. KASP, TaqMan and HRM markers are currently the most promising technologies for identifying crown rust resistance genes. The validated SCAR and STS markers for the Pc39, Pc68, Pc91, Pc94 genes are recommended as the most available for implementation in practical oat breeding. The results of recent studies on identifying loci of nonspecific resistance to P. coronata are also presented. In general, the use of DNA markers has significant potential for creating oat genotypes resistant to crown rust under present-day conditions. DNA markers of various types are recommended for practical use, in particular for pyramiding genes and increasing the resistance period of new cultivars. Introduction of DNA markers into oat breeding will increase with the growth of molecular genetic data and the improvement of technologies for identifying genes and loci associated with both race-specific and nonspecific resistance of oat to P. coronata.

AvenaPuccinia coronataгены устойчивостимолекулярные маркерыAvenaPuccinia coronataresistance genesmolecular markersработа выполнена в рамках Государственного задания ФГБНУ «Федеральный аграрный научный центр Северо-Востока имени Н.В. Рудницкого» (тема FNWE-2022-0001 «Разработка подходов к внедрению в процесс селекции зерновых культур постгеномных методов в целях повышения их адаптивности и создания устойчивых микробно-растительных ассоциаций») и при поддержке гранта (регистрационный номер в Единой государственной информационной системе учета научно-исследовательских, опытно-конструкторских и технических работ гражданского назначения 122022700120-9). Авторы благодарят рецензентов за их вклад в экспертную оценку этой работы.the work was carried out within the framework of the State Task for the Federal Agricultural Research Center of the North-East named N.V. 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The authors declare that there are no conflicts of interest present.