vir-nwТруды по прикладной ботанике, генетике и селекцииProceedings on applied botany, genetics and breeding2227-88342619-0982N.I. Vavilov All-Russian Institute of Plant Genetic Resources10.30901/2227-8834-2022-1-127-134vir-nw-1198Research ArticleГЕНЕТИКА КУЛЬТУРНЫХ РАСТЕНИЙ И ИХ ДИКИХ РОДИЧЕЙGENETICS OF CULTIVATED PLANTS AND THEIR WILD RELATIVESИзучение влияния чужеродных транслокаций на показатели андрогенеза in vitro у линий мягкой пшеницы (Triticum aestivum L.)The influence of combinations of alien translocations on in vitro androgenesis in spring common wheat (Triticum aestivum L.)https://orcid.org/0000-0002-3341-5080ТимоноваЕ. М.TimonovaE. M.

Екатерина Михайловна Тимонова, кандидат биологических наук, научный сотрудник

630090, Новосибирск, пр. акад. Лаврентьева, 10

Ekaterina M. Timonova, Cand. Sci. (Biology), Researcher

10 Lavrentyeva Ave., Novosibirsk 630090

eegorova@bionet.nsc.ruhttps://orcid.org/0000-0002-8460-6119АдонинаИ. Г.AdoninaI. G.

Ирина Григорьевна Адонина, кандидат биологических наук, научный сотрудник

630090, Новосибирск, пр. акад. Лаврентьева, 10

Irina G. Adonina, Cand. Sci. (Biology), Researcher

10 Lavrentyeva Ave., Novosibirsk 630090

adonina@bionet.nsc.ruhttps://orcid.org/0000-0001-8590-847XСалинаЕ. А.SalinaE. A.

Елена Артёмовна Салина, доктор биологических наук, профессор, зав. лабораторией, руководитель отделения

630090, Новосибирск, пр. акад. Лаврентьева, 10

Elena A. Salina, Dr. Sci. (Biology), Professor, Head of a Laboratory, Head of a Department

10 Lavrentyeva Ave., Novosibirsk 630090

salina@bionet.nsc.ruФедеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук; Курчатовский геномный центр Института цитологии и генетики Сибирского отделения Российской академии наукРоссияInstitute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences; Kurchatov Genomic Center of the Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of SciencesRussian FederationКурчатовский геномный центр Института цитологии и генетики Сибирского отделения Российской академии наукРоссияKurchatov Genomic Center of the Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of SciencesRussian Federation2022180420221831127134Copyright © Тимонова Е.М., Адонина И.Г., Салина Е.А., 20222022Тимонова Е.М., Адонина И.Г., Салина Е.А.Timonova E.M., Adonina I.G., Salina E.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://elpub.vir.nw.ru/jour/article/view/1198

Актуальность. Перенос в геном пшеницы генетического материала от диких и культурных злаков, стабилизация полученного материала и создание сорта на его основе является длительным процессом. Применение технологий удвоенных гаплоидов значительно его ускоряет. Для эффективного использования дигаплоидных технологий необходима информация о влиянии чужеродных транслокаций на результативность этого процесса.

Цель настоящей работы – изучить реакцию генотипов мягкой пшеницы, содержащих разные комбинации чужеродных транслокаций, на андрогенез in vitro.Материалы и методы. В работе использовался метод получения дигаплоидов из культуры пыльников пшеницы ‘Новосибирская 16’; линии Велют 991 – донора транслокаций T1RS.1BL от ржи и T5BS.5BL-5SL от Aegilops speltoides Tausch; четырех линий поколения F3 от их скрещивания 10-7, 14-8, 15-8, 15-12, различающихся содержанием транслокаций. Эффективность андрогенеза оценивалась по числу эмбриоидов, альбиносных и зеленых растений на 100 пыльников.Результаты. Наиболее высокие показатели отмечены для линий Велют 991, 10-7 и 14-8, характеризующихся присутствием в геноме T1RS.1BL. Так, частота регенерации зеленых растений для них составила 8,6, 3,6 и 10,1% соответственно. Значения показателей андрогенеза у линии 15-12 с T5BS.5BL-5SL были значительно ниже и практически не отличались от соответствующих значений для линии 15-8 без чужеродного материала.Заключение. Установлено положительное влияние T1RS.1BL, в том числе в сочетании с T5BS.5BL-5SL, на индукцию эмбриогенеза и регенерацию зеленых растений. Показано, что наличие только одной транслокации T5BS.5BL-5SL не влияло на эффективность андрогенеза.

Background. The basic approach to the production of new common wheat genotypes involving introgressive hybridization entails a long-term process. Doubled haploid production could accelerate it. However, this method is not widely used in breeding programs due to its main limitation: the genotype dependence. Due to genetic differences between wheat and related species, it was assumed that alien genetic materials are different in their capacity to affect androgenesis. The effect of alien translocations on androgenesis has been shown earlier. The aim of this study was to develop a set of DH wheat lines containing a wheat-alien translocation in the genome and study the effect of alien translocations on androgenesis of anther culture in such lines.Materials and methods. The plant material included: the spring wheat cultivar ‘Novosibirskaya 16’, line Velut 991 carrying wheat-alien translocations 1RS.1BL from rye and 5BS.5BL-5SL from Aegilops speltoides Tausch, and four hybrid F3  generation lines (10-7, 14-8, 15-8, 15-12) from their crossing, differing in the content of alien translocations.Results. It was shown that parameters of androgenesis such as the number of embryo-like structures per 100 anthers, the number of albino regenerants per 100 anthers, and the number of green regenerants per 100 anthers varied depending on the line. The best -responding lines Velut 991, 10-7 and 14-8 are characterized by the presence of a 1RS.1BL wheat-rye translocation chromosome. Regeneration frequency of green plants was recorded to be 8,6%, 3,6% and 10,1% respectively. The values of the parameters for lines 15-12 (carrying 5BS.5BL-5SL translocation) and 15-8 (without translocations) did not differ significantly.Conclusion. Therefore, it can be concluded that the presence of the introgressive fragment of chromosome 5S did not affect the efficiency of androgenesis and the short shoulder of chromosome 1R carries genes that stimulated androgenesis in anther culture.

удвоенные гаплоидыэмбриоидыкультура пыльниковспонтанное удвоение числа хромосомdoubled haploidsembryo-like structuresanther culturespontaneous chromosome doublingработа была поддержана Курчатовским геномным центром ИЦиГ СО РАН (соглашение № 075–15– 2019-1662). Размножение растений проводилось в ЦКП «Лаборатория искусственного выращивания растений» (поддерживается Министерством науки и высшего образования, проект FWNR-2022-0017). Авторы благодарят рецензентов за их вклад в экспертную оценку этой работы.this work was done within the framework of State Task assigned to the Kurchatov Genomic Center of the Institute of Cytology and Genetics, SB RAS (No. 075-15-2019-1662). Multiplication of plants was carried out in the Laboratory of Artificial Plant Growth (supported by the Ministry of Science and Higher Education, Project FWNR-2022-0017). The authors thank the reviewers for their contribution to the peer review of this work.ReferencesАдонина И.Г., Тимонова Е.М., Салина Е.А. 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The authors declare that there are no conflicts of interest present.