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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">vir-nw</journal-id><journal-title-group><journal-title xml:lang="ru">Труды по прикладной ботанике, генетике и селекции</journal-title><trans-title-group xml:lang="en"><trans-title>Proceedings on applied botany, genetics and breeding</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2227-8834</issn><issn pub-type="epub">2619-0982</issn><publisher><publisher-name>N.I. Vavilov All-Russian Institute of Plant Genetic Resources</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.30901/2227-8834-2021-3-125-136</article-id><article-id custom-type="elpub" pub-id-type="custom">vir-nw-1036</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ГЕНЕТИКА КУЛЬТУРНЫХ РАСТЕНИЙ И ИХ ДИКИХ РОДИЧЕЙ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>GENETICS OF CULTIVATED PLANTS AND THEIR WILD RELATIVES</subject></subj-group></article-categories><title-group><article-title>Устойчивость синтетической гексаплоидной пшеницы к возбудителю бурой ржавчины</article-title><trans-title-group xml:lang="en"><trans-title>Resistance of synthetic hexaploid wheat to the leaf rust pathogen</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Хакимова</surname><given-names>А. Г.</given-names></name><name name-style="western" xml:lang="en"><surname>Khakimova</surname><given-names>A. G.</given-names></name></name-alternatives><bio xml:lang="ru"><p>190000 Россия, г. Санкт-Петербург, ул. Б. Морская, 42, 44</p></bio><bio xml:lang="en"><p>42, 44 Bolshaya Morskaya Street, St. Petersburg 190000, Russia</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-7948-0307</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Гультяева</surname><given-names>Е. И.</given-names></name><name name-style="western" xml:lang="en"><surname>Gultyaeva</surname><given-names>E. I.</given-names></name></name-alternatives><bio xml:lang="ru"><p>196608 Россия, г. Санкт-Петербург, Пушкин, ш. Подбельского, 3</p></bio><bio xml:lang="en"><p>3 Podbelskogo Hwy., Pushkin, St. Petersburg 196608, Russia</p></bio><email xlink:type="simple">eigultyaeva@gmail.com</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Митрофанова</surname><given-names>О. П.</given-names></name><name name-style="western" xml:lang="en"><surname>Mitrofanova</surname><given-names>O. P.</given-names></name></name-alternatives><bio xml:lang="ru"><p>190000 Россия, г. Санкт-Петербург, ул. Б. Морская, 42, 44</p></bio><bio xml:lang="en"><p>42, 44 Bolshaya Morskaya Street, St. Petersburg 190000, Russia</p></bio><email xlink:type="simple">o.mitrofanova@vir.nw.ru</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>Федеральный исследовательский центр Всероссийский институт генетических ресурсов растений имени Н.И.Вавилова</institution><country>Россия</country></aff><aff xml:lang="en"><institution>N.I. Vavilov All-Russian Institute of Plant Genetic Resources</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru"><institution>Всероссийский научно-исследовательский институт защиты растений</institution><country>Россия</country></aff><aff xml:lang="en"><institution>All-Russian Research Institute of Plant Protection</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2021</year></pub-date><pub-date pub-type="epub"><day>07</day><month>10</month><year>2021</year></pub-date><volume>182</volume><issue>3</issue><fpage>125</fpage><lpage>136</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Хакимова А.Г., Гультяева Е.И., Митрофанова О.П., 2021</copyright-statement><copyright-year>2021</copyright-year><copyright-holder xml:lang="ru">Хакимова А.Г., Гультяева Е.И., Митрофанова О.П.</copyright-holder><copyright-holder xml:lang="en">Khakimova A.G., Gultyaeva E.I., Mitrofanova O.P.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://elpub.vir.nw.ru/jour/article/view/1036">https://elpub.vir.nw.ru/jour/article/view/1036</self-uri><abstract><sec><title>Актуальность</title><p>Актуальность. Один из перспективных источников обогащения генофонда мягкой пшеницы (Triticum aestivum L.) новыми аллелями генов – синтетическая гексаплоидная пшеница (СГП), или аллополиплоиды от скрещивания тетраплоидных пшениц (2n = 4x = 28, BBAA) с образцами Aegilops tauschii Coss. (2n = 2x = 14, DD) и последующего удвоения числа хромосом у гибридов. Целями исследования были: оценка образцов синтетической гексаплоидной пшеницы (СГП) по устойчивости к популяциям возбудителя бурой ржавчины, собранным на территории России; генотипирование образцов; на основе анализа литературы обобщение сведений об устойчивости изученных образцов к другим вредоносным болезням и вредителям.</p></sec><sec><title>Материалы и методы</title><p>Материалы и методы. Устойчивость 36 образцов СГП из коллекции ВИР к популяциям Puccinia triticina Erikss. оценивали по методикам ВИЗР в лабораторных условиях и в поле на искусственном инфекционном фоне. Для идентификации Lr-генов применяли фитопатологический тест и ПЦР-маркеры.</p></sec><sec><title>Результаты и заключение</title><p>Результаты и заключение. Образцы СГП охарактеризованы по устойчивости к российским популяциям возбудителя бурой ржавчины. Выявлены источники устойчивости в фазе проростков и взрослого растения. Фитопатологическим тестом у трех образцов обнаружен ген устойчивости Lr23, ПЦР-маркер гена Lr21=Lr40 присутствовал у 11 образцов, Lr39=Lr41 – у 19, Lr22a – у трех. При этом образцы к-65496, к-65515, к-65517 имели одновременно Lr21=Lr40 и Lr39=Lr41, а к-65497, к-65503 и к-65508 – Lr22a и Lr39=Lr41. Анализ литературы показал, что многие из изученных образцов СГП устойчивы к другим вредоносным болезням и насекомым-вредителям и представляют интерес для дальнейшего изучения и возможного использования в отечественной селекции.</p></sec></abstract><trans-abstract xml:lang="en"><sec><title>Background</title><p>Background. One of the promising sources for enrichment of the common wheat (Triticum aestivum L.) gene pool with new alleles is synthetic hexaploid wheat (SHW), or allopolyploids from crossing tetraploid wheats (2n = 4x = 28, BBAA) with accessions of Aegilops tauschii Coss. (2n = 2x = 14, DD), and subsequent doubling of the chromosome number in the hybrids. Objectives of the study were to evaluate the SHW accessions from the VIR collection for resistance to Puccinia triticina Erikss. populations collected in Russia; genotype the accessions; and summarize information from the published sources concerning the resistance of the studied accessions to other harmful diseases and pests.</p></sec><sec><title>Materials and methods</title><p>Materials and methods. Resistance of 36 SHW accessions from the VIR collection to the populations of P. triticina was assessed in the laboratory and in the field, under artificial infection pressure, using the techniques developed by the Institute of Plant Protection. A phytopathological test and PCR markers were used to identify the Lr genes.</p></sec><sec><title>Results and conclusion</title><p>Results and conclusion. The SHW accessions were characterized according to their resistance to the Russian populations of the wheat leaf rust pathogen. The sources of resistance in the phase of emergence and in adult plants were identified. The phytopathological test isolated three accessions with Lr23; the PCR marker of Lr21=Lr40 was found in 11 accessions, Lr39=Lr41 in 19, and Lr22a in 3. At the same time, k-65496, k-65515 and k-65517 had si multaneously Lr21=Lr40 and Lr39=Lr41, while k-65497, k-65503 and k-65508 had Lr22a and Lr39=Lr41. The analysis of published data showed that many of the studied SHW accessions were also resistant to other harmful diseases and insect pests, so they are of interest for further studying and possible use in domestic breeding.</p></sec></trans-abstract><kwd-group xml:lang="ru"><kwd>D-геном</kwd><kwd>Triticum aestivum</kwd><kwd>Aegilops tauschii</kwd><kwd>фитопатологический тест</kwd><kwd>ПЦР-маркер</kwd><kwd>Lr-гены</kwd><kwd>ювенильная устойчивость</kwd><kwd>долговременная устойчивость</kwd><kwd>источники устойчивости</kwd></kwd-group><kwd-group xml:lang="en"><kwd>the D genome</kwd><kwd>Triticum aestivum</kwd><kwd>Aegilops tauschii</kwd><kwd>phytopathological test</kwd><kwd>PCR marker</kwd><kwd>Lr genes</kwd><kwd>juvenile resistance</kwd><kwd>long-term resistance</kwd><kwd>sources of resistance</kwd></kwd-group><funding-group><funding-statement xml:lang="ru">Работа выполнена в рамках государственных заданий по тематическим планам: ВИР, проект № 0662-2019-0006 «Поиск, поддержание жизнеспособности и раскрытие потенциала наследственной изменчивости мировой коллекции зерновых и крупяных культур ВИР для развития оптимизированного генбанка и рационального использования в селекции и растениеводстве»; ВИЗР, проект № 0665-2019-0015 «Грибы-патогены экономически значимых растений в России: разнообразие, методы идентификации и мониторинга, взаимоотношения с растениями-хозяевами».</funding-statement><funding-statement xml:lang="en">The research was performed within the frameworks of the State Tasks according to the theme plans of: VIR, Project No. 0662-2019-0006 “Search for and viability maintenance, and disclosing the potential of hereditary variation in the global collection of cereal and groat crops at VIR for the development of an optimized genebank and its sustainable utilization in plant breeding and crop production”; VIZR, Project No. 0665-2019-0015 “Fungal pathogens of economically significant plants in Russia: diversity, methods of identification and monitoring, relationships with host plants”.</funding-statement></funding-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Aktar-Uz-Zaman M., Tuhina-Khatun M., Hanafi M.M., Sahebi M. 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