<?xml version="1.0" encoding="UTF-8"?>
<!DOCTYPE article PUBLIC "-//NLM//DTD JATS (Z39.96) Journal Publishing DTD v1.3 20210610//EN" "JATS-journalpublishing1-3.dtd">
<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">vir-nw</journal-id><journal-title-group><journal-title xml:lang="ru">Труды по прикладной ботанике, генетике и селекции</journal-title><trans-title-group xml:lang="en"><trans-title>Proceedings on applied botany, genetics and breeding</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2227-8834</issn><issn pub-type="epub">2619-0982</issn><publisher><publisher-name>N.I. Vavilov All-Russian Institute of Plant Genetic Resources</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.30901/2227-8834-2021-3-30-36</article-id><article-id custom-type="elpub" pub-id-type="custom">vir-nw-1026</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ИЗУЧЕНИЕ И ИСПОЛЬЗОВАНИЕ ГЕНЕТИЧЕСКИХ РЕСУРСОВ РАСТЕНИЙ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>STUDYING AND UTILIZATION OF PLANT GENETIC RESOURCES</subject></subj-group></article-categories><title-group><article-title>Подбор концентрации осмотика ПЭГ 6000 для определения засухоустойчивости генотипов проса в период прорастания семян</article-title><trans-title-group xml:lang="en"><trans-title>Identification of PEG 6000 concentrations for assessing drought resistance in millet genotypes during the seed germination phase</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-1234-8368</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Горлачева</surname><given-names>О. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Gorlachova</surname><given-names>O. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>61060 Украина, г. Харьков, пр. Московский, 142</p></bio><bio xml:lang="en"><p>142 Moskovskyi Ave., Kharkiv 61060, Ukraine</p></bio><email xlink:type="simple">dr_forester@ukr.net</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-7835-822X</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Горбачева</surname><given-names>С. Н.</given-names></name><name name-style="western" xml:lang="en"><surname>Gorbachova</surname><given-names>S. N.</given-names></name></name-alternatives><bio xml:lang="ru"><p>61060 Украина, г. Харьков, пр. Московский, 142</p></bio><bio xml:lang="en"><p>142 Moskovskyi Ave., Kharkiv 61060, Ukraine</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-3565-1033</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Лютенко</surname><given-names>В. С.</given-names></name><name name-style="western" xml:lang="en"><surname>Lutenko</surname><given-names>V. S.</given-names></name></name-alternatives><bio xml:lang="ru"><p>61060 Украина, г. Харьков, пр. Московский, 142</p></bio><bio xml:lang="en"><p>142 Moskovskyi Ave., Kharkiv 61060, Ukraine</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-1466-1294</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Анцыферова</surname><given-names>О. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Anceferova</surname><given-names>O. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>61060 Украина, г. Харьков, пр. Московский, 142</p></bio><bio xml:lang="en"><p>142 Moskovskyi Ave., Kharkiv 61060, Ukraine</p></bio><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>Институт растениеводства имени В.Я. Юрьева Национальной академии аграрных наук Украины</institution><country>Украина</country></aff><aff xml:lang="en"><institution>Plant Production Institute named after V.Ya. Yuriev, National Academy of Agrarian Sciences of Ukraine</institution><country>Ukraine</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2021</year></pub-date><pub-date pub-type="epub"><day>06</day><month>10</month><year>2021</year></pub-date><volume>182</volume><issue>3</issue><fpage>30</fpage><lpage>36</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Горлачева О.В., Горбачева С.Н., Лютенко В.С., Анцыферова О.В., 2021</copyright-statement><copyright-year>2021</copyright-year><copyright-holder xml:lang="ru">Горлачева О.В., Горбачева С.Н., Лютенко В.С., Анцыферова О.В.</copyright-holder><copyright-holder xml:lang="en">Gorlachova O.V., Gorbachova S.N., Lutenko V.S., Anceferova O.V.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://elpub.vir.nw.ru/jour/article/view/1026">https://elpub.vir.nw.ru/jour/article/view/1026</self-uri><abstract><sec><title>Актуальность</title><p>Актуальность. Использование для определения засухоустойчивости генотипов проса осмотика полиэтиленгликоля (ПЭГ) 6000 по сравнению с растворами сахарозы является наиболее простым и экономически оправданным. Применение ПЭГ 6000 при полулетальной дозе (LD50) для отбора на засухоустойчивость проса на ранних этапах онтогенеза в наибольшей степени дифференцирует генотипы по изучаемому признаку, позволяет проводить оценку в соответствии с международными стандартами и дает возможность сравнения полученных результатов с данными зарубежных исследований.</p></sec><sec><title>Материалы и методы</title><p>Материалы и методы. Для проведения исследования в качестве исходного материала использовали сорта ‘Омрияне’, ‘Харьковское 57’, ‘Константиновское’, ‘Слобожанское’ и образец IR 5. Готовили пять концентраций растворов ПЭГ 6000: 11,5%, 15,3%, 19,6%, 23,5%, 28,9%, 0,0% (контроль). Проращивали по 50 семян в трехкратной повторности. На шестые сутки снимали показания всхожести семян проса. Для теоретического обоснования подбора оптимальной концентрации осмотика применяли регрессионный метод оценки LD50 (полулетальной дозы) В. Б. Прозоровского.</p></sec><sec><title>Результаты и выводы</title><p>Результаты и выводы. На степень прорастания семян проса раствор ПЭГ 6000 в концентрации от 15,3% до 28,9% оказывал негативное влияние. Резкое падение всхожести семян проса наблюдали при 23,5-процентной концентрации ПЭГ 6000 – всхожесть семян у всех сортообразцов (кроме IR 5) была ниже 50%. Результаты расчета по логистической кривой Ферхюльста и пробит-анализу методом В. Б. Прозоровского показали, что среднее значение концентрации LD50 раствора ПЭГ 6000 у всех изучаемых образцов равнялось 23,03%. Таким образом, в результате анализа наших экспериментальных данных и их статистических обработок мы рекомендуем использовать 23,0-процентную концентрацию LD50 раствора ПЭГ 6000 как наиболее дифференцирующую просо по засухоустойчивости в фазе прорастания.</p></sec></abstract><trans-abstract xml:lang="en"><sec><title>Background</title><p>Background. Using LD50 concentrations of the osmotic PEG 6000 to select millet genotypes during seed germination for drought resistance breeding is the best way of millet genotype differentiation according to the studied trait.</p></sec><sec><title>Materials and methods</title><p>Materials and methods. The millet cultivars ‘Omriyane’, ‘Kharkovskoe 57’, ‘Konstantinovskoe’, ‘Slobozhanskoe’, and the accession IR 5 were selected as the test material. Water stress was applied through five concentrations of PEG (6000 MW): 11.5%, 15.3%, 19.6%, 23.5%, 28.9%, and 0.0% (control). On the sixth day of incubation, millet seed germination was measured. The regression method for assessing LD50 (half-lethal dose) by V. B. Prozorovskii was used to theoretically substantiate the selection of an optimal osmotic concentration, which would be most accurate in identifying the level of drought tolerance in millet genotypes during seed germination.</p></sec><sec><title>Results and conclusions</title><p>Results and conclusions: The 15.3% to 28.9% concentrations of PEG 6000 solutions had a negative effect on seed germination of millet genotypes. A strongly suppressed seed germination rate was observed at the PEG concentration of 23.5%: germination percentage in all cultivars (except for IR 5, with 56.0%) fell below 50%. Calculations according to the Verhulst logistic curve and the probit analysis by V. B. Prozorovskii’s technique showed that the mean LD50 concentration of PEG 6000 solutions for all studied genotypes was 23.03%. Thus, as a result of the analysis of our experimental data and their statistical processing, we recommended the 23.0% concentration of PEG 6000 solution as the most differentiating in terms of drought resistance in the millet seed germination phase.</p></sec></trans-abstract><kwd-group xml:lang="ru"><kwd>Panicum miliaceum L.</kwd><kwd>устойчивость к засухе</kwd><kwd>LD50</kwd><kwd>логистическая кривая</kwd><kwd>пробит-анализ</kwd></kwd-group><kwd-group xml:lang="en"><kwd>Panicum miliaceum L.</kwd><kwd>drought resistance</kwd><kwd>LD50</kwd><kwd>logistic curve</kwd><kwd>probit analysis</kwd></kwd-group><funding-group><funding-statement xml:lang="ru">Работа выполнена в рамках государственного задания 13.00.10.01 Ф согласно рабочей программе на 2021 год «Разработка методологических основ селекции проса на засухоустойчивость, выделение источников и создание урожайных сортов проса с улучшенным качеством зерна и устойчивостью к стрессовым факторам среды».</funding-statement><funding-statement xml:lang="en">The work was carried out within the framework of State Task 13.00.10.01 F according to the working program for 2021 “Development of methodological foundations for breeding of millet for drought resistance, identification of sources, and development of high-yielding millet cultivars with improved grain quality and resistance to environmental stressors”.</funding-statement></funding-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Бабич П.Н., Чубенко А.В., Лапач С.Н. Применение пробит-анализа в токсикологии и фармакологии с использованием программы Microsoft Excel для оценки фармакологической активности при альтернативной форме учета реакции. Сучасні проблеми токсикології. 2003;(4):80-88.</mixed-citation><mixed-citation xml:lang="en">Babich P.N., Chubenko P.N., Lapach S.N. Application of probit analysis in toxicology and pharmacology using the Microsoft Excel program for assessing pharmacological activity in an alternative form of reaction accounting. Modern Problems of Toxicology. 2003;(4):80-88. [in Russian].</mixed-citation></citation-alternatives></ref><ref id="cit2"><label>2</label><citation-alternatives><mixed-citation xml:lang="ru">Bayoumi T.Y., Eid M.H., Metwali E.M. Application of physiological and biochemical indices as a screening technique for drought tolerance in wheat genotypes. African Journal of Biotechnology. 2008;7(14):2341-2352.</mixed-citation><mixed-citation xml:lang="en">Bayoumi T.Y., Eid M.H., Metwali E.M. Application of physiological and biochemical indices as a screening technique for drought tolerance in wheat genotypes. African Journal of Biotechnology. 2008;7(14):2341-2352.</mixed-citation></citation-alternatives></ref><ref id="cit3"><label>3</label><citation-alternatives><mixed-citation xml:lang="ru">Blum A. Drought resistance, water use efficiency, and yield potential – are they compatible, dissonant, or mutually exclusive? Australian Journal of Agricultural Research. 2005;56(11):1159-1168. DOI: 10.1071/AR05069</mixed-citation><mixed-citation xml:lang="en">Blum A. Drought resistance, water use efficiency, and yield potential – are they compatible, dissonant, or mutually exclusive? Australian Journal of Agricultural Research. 2005;56(11):1159-1168. DOI: 10.1071/AR05069</mixed-citation></citation-alternatives></ref><ref id="cit4"><label>4</label><citation-alternatives><mixed-citation xml:lang="ru">Бухаров А.Ф., Балеев Д.Н., Бухаро ва А.Р. Кинетика прорастания семян. Методы исследования и параметры. Известия Тимирязевской сельскохозяйственной академии. 2017;(2):5-19.</mixed-citation><mixed-citation xml:lang="en">Bukharov A.F., Baleyev D.N., Bukharova A.R. Kinetics of seed germination. Research methods and parameters. Izvestiya of Timiryazev Agricultural Academy. 2017;(2):5-19. [in Russian]</mixed-citation></citation-alternatives></ref><ref id="cit5"><label>5</label><citation-alternatives><mixed-citation xml:lang="ru">Cheng B., Xie Z. Advance in water-saving culture of proso millet (Panicum miliaceum L.). In: Y. Chai, B. Feng (eds). Advances in Broomcorn Millet Research: Proceedings of the 1st International Symposium on Broomcorn Millet (Yangling, August 25–31, 2012). Yangling, Shaanxi: Northwest A&amp;F University Press; 2012. p.203-209.</mixed-citation><mixed-citation xml:lang="en">Cheng B., Xie Z. Advance in water-saving culture of proso millet (Panicum miliaceum L.). In: Y. Chai, B. Feng (eds). Advances in Broomcorn Millet Research: Proceedings of the 1st International Symposium on Broomcorn Millet (Yangling, August 25–31, 2012). Yangling, Shaanxi: Northwest A&amp;F University Press; 2012. p.203-209.</mixed-citation></citation-alternatives></ref><ref id="cit6"><label>6</label><citation-alternatives><mixed-citation xml:lang="ru">Чернышова С.В. Определение относительной засухоустойчивости образцов проса способом проращивания семян в растворах сахарозы (Методические указания). Ленинград; 1987.</mixed-citation><mixed-citation xml:lang="en">Chernyshova S.V. Determination of relative drought resistance in millet accessions by germinating seeds in sucrose solutions (Guidelines) (Opredeleniye otnositelnoy zasukhoustoychivosti obraztsov prosa sposobom prorashchivaniya semyan v rastvorakh sakharozy [Metodicheskiye ukazaniya]). Leningrad; 1987. [in Russian]</mixed-citation></citation-alternatives></ref><ref id="cit7"><label>7</label><citation-alternatives><mixed-citation xml:lang="ru">Demuyakor B., Galyuon I., Kyereh S., Ahmed M. Evaluation of agronomic performance of drought–tolerant QTL introgression hybrids of millet (Pennisetum glaucum (L.) R. Br.) in the Guinea Savannah zone of Ghana. International Journal of Agriculture Sciences. 2013;5(1):354-358. Available from: https://bioinfopublication.org/files/articles/5_1_4_IJAS.pdf [accessed Mar. 10, 2021].</mixed-citation><mixed-citation xml:lang="en">Demuyakor B., Galyuon I., Kyereh S., Ahmed M. Evaluation of agronomic performance of drought–tolerant QTL introgression hybrids of millet (Pennisetum glaucum (L.) R. Br.) in the Guinea Savannah zone of Ghana. International Journal of Agriculture Sciences. 2013;5(1):354-358. Available from: https://bioinfopublication.org/files/articles/5_1_4_IJAS.pdf [accessed Mar. 10, 2021].</mixed-citation></citation-alternatives></ref><ref id="cit8"><label>8</label><citation-alternatives><mixed-citation xml:lang="ru">Горлачова О.В.Особливості формування структури врожаю сортозразків проса в селекції на посухостійкість. Вісник ХНАУ. Серія «Рослинництво, селекція і насінництво, плодоовочівництво». 2011;1:121-127.</mixed-citation><mixed-citation xml:lang="en">Gorlachova O.V. The effects of drought stress on growth parameters and grain yields of millet. The Bulletin of Kharkiv National Agrarian University. Series: Plant Production, Breeding, Seed Production, Fruit and Vegetable Production. 2011;1:121-127. [in Ukraine]</mixed-citation></citation-alternatives></ref><ref id="cit9"><label>9</label><citation-alternatives><mixed-citation xml:lang="ru">Govindaraj M., Shanmugasundaram P., Sumathi P., Muthiah A.R. Simple, rapid and cost effective screening method for drought resistant breeding in pearl millet. Electronic Journal of Plant Breeding. 2010;1(4):590-599.</mixed-citation><mixed-citation xml:lang="en">Govindaraj M., Shanmugasundaram P., Sumathi P., Muthiah A.R. Simple, rapid and cost effective screening method for drought resistant breeding in pearl millet. Electronic Journal of Plant Breeding. 2010;1(4):590-599.</mixed-citation></citation-alternatives></ref><ref id="cit10"><label>10</label><citation-alternatives><mixed-citation xml:lang="ru">Keshavars L., Farahbakhsh H., Golkar P. The effect of drought stress and super absorbent polymer on morph-physiological traits of pearl millet (Pennisetum glaucum). International Research Journal of Applied and Basic Sciences. 2012;3(1):148-154. Available from: https://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.177.7751&amp;rep=rep1&amp;type=pdf [accessed Mar. 04, 2021].</mixed-citation><mixed-citation xml:lang="en">Keshavars L., Farahbakhsh H., Golkar P. The effect of drought stress and super absorbent polymer on morph-physiological traits of pearl millet (Pennisetum glaucum). International Research Journal of Applied and Basic Sciences. 2012;3(1):148-154. Available from: https://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.177.7751&amp;rep=rep1&amp;type=pdf [accessed Mar. 04, 2021].</mixed-citation></citation-alternatives></ref><ref id="cit11"><label>11</label><citation-alternatives><mixed-citation xml:lang="ru">Коросов А.В. Имитационное моделирование в среде MS (на примерах из экологии): Монография. Петрозаводск: Петрозаводский государственный университет; 2002</mixed-citation><mixed-citation xml:lang="en">Korosov A.V. Simulation modeling in the MS Excel environment (on examples from ecology): Monograph (Imitatsionnoye modelirovaniye v srede MS Excel [na primerakh iz ekologii]: Monografiya). Petrozavodsk: Petrozavodsk State University; 2002. [in Russian]</mixed-citation></citation-alternatives></ref><ref id="cit12"><label>12</label><citation-alternatives><mixed-citation xml:lang="ru">Коросов А.В., Каланкина Н.М. Количественные методы экологической токсикологии. Петрозаводск; 2003.</mixed-citation><mixed-citation xml:lang="en">Korosov A.V., Kalankina N.M. Quantitative methods of environmental toxicology (Kolichestvennye metody ekologicheskoy toksikologii). Petrozavodsk; 2003. [in Russian]</mixed-citation></citation-alternatives></ref><ref id="cit13"><label>13</label><citation-alternatives><mixed-citation xml:lang="ru">Лакин Г.Ф. Биометрия. Москва; 1990.</mixed-citation><mixed-citation xml:lang="en">Lakin G.F. Biometrics (Biometriya). Мoscow; 1990. [in Russian]</mixed-citation></citation-alternatives></ref><ref id="cit14"><label>14</label><citation-alternatives><mixed-citation xml:lang="ru">Mitra J. Genetics and genetic improvement of drought resistance in crop plants. Current Science. 2001;80(6):758-763.</mixed-citation><mixed-citation xml:lang="en">Mitra J. Genetics and genetic improvement of drought resistance in crop plants. Current Science. 2001;80(6):758-763.</mixed-citation></citation-alternatives></ref><ref id="cit15"><label>15</label><citation-alternatives><mixed-citation xml:lang="ru">O’Donnell N.H., Møller B.L., Neale A.D., Hamill J.D., Blomstedt C.K., Gleadow R.M. Effects of PEG-induced osmotic stress on growth and dhurrin levels of forage sorghum. Plant Physiology and Biochemistry. 2013;73:83-92. DOI: 10.1016/j.plaphy.2013.09.001</mixed-citation><mixed-citation xml:lang="en">O’Donnell N.H., Møller B.L., Neale A.D., Hamill J.D., Blomstedt C.K., Gleadow R.M. Effects of PEG-induced osmotic stress on growth and dhurrin levels of forage sorghum. Plant Physiology and Biochemistry. 2013;73:83-92. DOI: 10.1016/j.plaphy.2013.09.001</mixed-citation></citation-alternatives></ref><ref id="cit16"><label>16</label><citation-alternatives><mixed-citation xml:lang="ru">Прозоровский В.Б. Статистическая обработка результатов фармакологических исследований. Психофармакология и биологическая наркология. 2007;7(3-4):2090-2120.</mixed-citation><mixed-citation xml:lang="en">Prozorovskii V.B. Statistic processing of data of pharmacological investigations. Psychopharmacology and Biological Narcology. 2007;7(3-4):2090-2120. [in Russian]</mixed-citation></citation-alternatives></ref><ref id="cit17"><label>17</label><citation-alternatives><mixed-citation xml:lang="ru">Radhouane L. Response of Tunisian autochthonous pearl millet (Pennisetum glaucum (L.) R. Br.) to drought stress induced by polyethylene glycol (PEG) 6000. African Journal of Biotechnology. 2007;6(9):1102-1105.</mixed-citation><mixed-citation xml:lang="en">Radhouane L. Response of Tunisian autochthonous pearl millet (Pennisetum glaucum (L.) R. Br.) to drought stress induced by polyethylene glycol (PEG) 6000. African Journal of Biotechnology. 2007;6(9):1102-1105.</mixed-citation></citation-alternatives></ref><ref id="cit18"><label>18</label><citation-alternatives><mixed-citation xml:lang="ru">Seghatoleslami M.J., Kafi M., Majini E. Effect of drought stress at different growth stages on yield and water use efficiency of five proso millet (Panicum miliaceum) genotypes. Journal of Crop Production and Processing. 2007:11(1):215-227.</mixed-citation><mixed-citation xml:lang="en">Seghatoleslami M.J., Kafi M., Majini E. Effect of drought stress at different growth stages on yield and water use efficiency of five proso millet (Panicum miliaceum) genotypes. Journal of Crop Production and Processing. 2007:11(1):215-227.</mixed-citation></citation-alternatives></ref><ref id="cit19"><label>19</label><citation-alternatives><mixed-citation xml:lang="ru">Tadele Z. Drought adaptation in millets. In: A. Shanker, C. Shanker (eds). Abiotic and Biotic Stress in Plants – Recent Advances and Future Perspectives. London: IntechOpen Ltd.; 2016. p.639-662. DOI: 10.5772/61929</mixed-citation><mixed-citation xml:lang="en">Tadele Z. Drought adaptation in millets. In: A. Shanker, C. Shanker (eds). Abiotic and Biotic Stress in Plants – Recent Advances and Future Perspectives. London: Intech- Open Ltd.; 2016. p.639-662. DOI: 10.5772/61929</mixed-citation></citation-alternatives></ref><ref id="cit20"><label>20</label><citation-alternatives><mixed-citation xml:lang="ru">Яшовский И.В. Селекция и семеноводство проса. Москва: Агропромиздат; 1987.</mixed-citation><mixed-citation xml:lang="en">Yashovskiy I.V. Millet breeding and seed production (Selektsiya i semenovodstvo prosa). Moscow: Agropromizdat; 1987. [in Russian]</mixed-citation></citation-alternatives></ref><ref id="cit21"><label>21</label><citation-alternatives><mixed-citation xml:lang="ru">Zhang P., Song H., Ma S., Cui W., Li C., Feng B. Physiological and biochemical indexes of drought resistance of broomcorn millet at seedling stage. In: Y. Chai, B. Feng (eds). Advances in Broomcorn Millet Research: Proceedings of the 1st International Symposium on Broomcorn Millet (Yangling, August 25–31, 2012). Yangling, Shaanxi: Northwest A&amp;F University Press; 2012. p.229-238.</mixed-citation><mixed-citation xml:lang="en">Zhang P., Song H., Ma S., Cui W., Li C., Feng B. Physiological and biochemical indexes of drought resistance of broomcorn millet at seedling stage. In: Y. Chai, B. Feng (eds). Advances in Broomcorn Millet Research: Proceedings of the 1st International Symposium on Broomcorn Millet (Yangling, August 25–31, 2012). Yangling, Shaanxi: Northwest A&amp;F University Press; 2012. p.229-238.</mixed-citation></citation-alternatives></ref></ref-list><fn-group><fn fn-type="conflict"><p>The authors declare that there are no conflicts of interest present.</p></fn></fn-group></back></article>
